Synergistic processing method for breeding trichogramma chilonis by using oak silkworm egg

A technology of Trichogramma borer and treatment method, which is applied in the field of crop biological control, can solve the problems of poor breeding quality and stability of Trichogramma borer, unfavorable breeding scale, etc., and achieve improved parasitic effect, low cost, and low cost. cheap effect

Inactive Publication Date: 2014-11-26
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under the current breeding method, the breeding quality and stability of Trichogramma borer are not good, which is not conducive to expanding the breeding scale

Method used

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  • Synergistic processing method for breeding trichogramma chilonis by using oak silkworm egg

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] egg surface pretreatment solution

[0019] Take the cauliflower ( Brassica oleracea var. botrytis ) appropriate amount of fresh inflorescence, remove surface unclean matter and strictly remove the rotten part, squeeze the remaining part with a juicer, filter through an 80-mesh screen, and obtain fresh juice as mother liquor, mother liquor with 0.02~0.03g / L benzene Dilute the aqueous ammonium bromide solution 20 to 25 times and stir evenly to obtain the egg surface pretreatment solution.

Embodiment 2

[0021] Under the lower multiplication ratio, the breeding indexes of the patented method are at excellent values.

[0022] according to figure 1 As shown, under artificial control conditions, tussah silkworm eggs were used to breed Trichogramma borer. The indoor temperature and humidity conditions were controlled at (25±2)°C and relative humidity (55±5)%.

[0023] Take the cauliflower ( Brassica oleracea var. botrytis ) appropriate amount of fresh inflorescence, remove surface impurities and strictly remove its rotten parts, squeeze the remaining part with a juicer, filter through an 80-mesh screen, and obtain fresh juice as mother liquor, mother liquor with 0.02g / L benzalkonium bromide The ammonium aqueous solution was diluted 20 times as the egg surface pretreatment solution; the tussah eggs that had been parasitized by Trichogramma melanogaster were used as parent eggs, and the fresh tussah eggs that had not been parasitized were used as offspring eggs; fresh tussah eggs...

Embodiment 3

[0032] Under the medium multiplication ratio, the breeding indicators of the patented method can still be stabilized in the excellent value area.

[0033] according to figure 1 As shown, under artificial control conditions, tussah silkworm eggs were used to breed Trichogramma borer. The indoor temperature and humidity conditions were controlled at (25±2)°C and relative humidity (55±5)%. The tussah silkworm eggs that have been parasitized by Trichogramma merrhizae are used as parent eggs, and the fresh tussah silkworm eggs that have not been parasitized are used as offspring eggs;

[0034] Take the cauliflower ( Brassica oleracea var. botrytis ) an appropriate amount of fresh inflorescence, remove surface impurities and strictly remove its rotten parts, squeeze the remaining part with a juicer, filter through an 80-mesh sieve, and obtain fresh juice as a mother liquor, and use 0.03g / L benzalkonium bromide for the mother liquor The ammonium aqueous solution was diluted 25 ti...

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Abstract

The invention discloses a synergistic processing method for breeding trichogramma chilonis by using an oak silkworm egg, and trichogramma chilonis is bred by using the oak silkworm egg under a manual controlled condition. The method comprises the following steps of soaking the surface of a fresh oak silkworm disemboweled egg by a preprocessed solution; taking out and airing the oak silkworm disemboweled egg to be used as a progeny egg for receiving the trichogramma chilonis; after the eclosion of the trichogramma chilonis from a parental egg, placing the parental egg and the progeny egg with the preprocessed surface together in a dark environment; after a parasitism link, removing the parental egg and the trichogramma chilonis; retaining the progeny egg, wherein the growth and eclosion of the trichogramma chilonis are completed under conditions that the wavelength of a blue light is 475-495 nm and the illumination intensity is 500-1000 lux. In large-scale reproduction, various development indexes of the trichogramma chilonis are better; the stability is stronger. Comparing the method with the traditional method, the parasitism rate can be increased by 4-26 percentage points; the trichogramma chilonis number of a single egg is moderate; the uniformity degree of samples in a sampling room is better; the eclosion rate can be increased by 0.2-4 percentage points; the parasitism rate is increased and maintained at a higher level; the trichogramma chilonis left rate is reduced by 1-3 percentage points; the uniformity degree of samples in the sampling room is better.

Description

technical field [0001] The invention discloses a synergistic treatment method for breeding Trichogramma borer with tussah eggs, belonging to the technical field of crop biological control. Background technique [0002] tussah silkworm ( Antheraea pernyi ) eggs to breed Trichogramma ( Trichogramma chilonis ) The current method is usually: clean the fresh tussah eggs that have not been parasitized and disinfect the surface of the eggs with water-soluble disinfectant, and then use them as offspring host eggs, and use the tussah eggs that have been parasitized by Trichogramma as the parent host eggs After Trichogramma japonica emerges from the parental eggs, the bees are picked up in the dark room for a certain period of time (24h, 48h or other), and then the offspring eggs are separated and placed in indoor conditions with natural lighting (bright period / dark period is about 14h / 10h), the indoor temperature and humidity conditions are usually controlled as follows: temperat...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): A01K67/033
Inventor李天昊阮长春张波杜文梅王秀梅万军刘显娇宋丽威
OwnerJILIN AGRICULTURAL UNIV