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Anaerobic indicator

An anaerobic indicator and buffering agent technology, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., and can solve the problems of insufficient color change and unsuitable storage

Inactive Publication Date: 2013-12-18
青岛高科技工业园海博生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Anaerobic culture is often used in the process of microbial detection. In anaerobic culture, it is necessary to indicate whether the environment is anaerobic. At present, melanin indicator and resazurin indicator are commonly used in China. Both indicators are liquid indicators. , need to prevent spilling during use, and need to be temporarily prepared, not suitable for storage, the color change range of melanin indicator is from colorless to blue, and the resazurin indicator is from pink to blue, and the transition between light blue and blue Purple has a certain influence on the result judgment
There are tablet indicators abroad, but the color change is not obvious enough

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] An anaerobic indicator, consisting of the following components by weight:

[0020] 100 parts of sodium thioglycolate;

[0021] Meilan 10 parts;

[0022] 100 parts of buffer;

[0023] 5 parts of phenol red;

[0024] 100 parts of agar.

[0025] Production steps: Except for agar, the rest of the ingredients are mixed into mother liquor, and then mixed according to the proportion, after mixing, add agar, boil and stir for about 5 minutes, until the agar is completely dissolved, pay attention to replenishing water during the period, until the liquid turns red completely, and distribute it while it is hot. In the ampoule, each bottle can be filled with 0.5-1 ml as required, and can be sealed in an oxygen-free environment, or placed for 30 days after sealing, to consume the remaining oxygen in the ampoule and reach an anaerobic state.

Embodiment 2

[0027] An anaerobic indicator, composed of the following components by weight:

[0028] 500 parts of sodium thioglycolate;

[0029] Meilan 60 parts;

[0030] 300 parts of buffer;

[0031] 40 parts of phenol red;

[0032] 260 parts of agar.

[0033] Production steps: Except for agar, the rest of the ingredients are mixed into mother liquor, and then mixed according to the proportion, after mixing, add agar, boil and stir for about 5 minutes, until the agar is completely dissolved, pay attention to replenishing water during the period, until the liquid turns red completely, and distribute it while it is hot. In the ampoule, each bottle can be filled with 0.5-1 ml as required, and can be sealed in an oxygen-free environment, or placed for 30 days after sealing, to consume the remaining oxygen in the ampoule and reach an anaerobic state.

Embodiment 3

[0035] An anaerobic indicator, consisting of the following components by weight:

[0036] Sodium thioglycolate 1000 parts;

[0037] Meilan 100 parts;

[0038] 500 parts of buffer;

[0039] 100 parts of phenol red;

[0040] 500 parts of agar.

[0041] Production steps: Except for agar, the rest of the ingredients are mixed into mother liquor, and then mixed according to the proportion, after mixing, add agar, boil and stir for about 5 minutes, until the agar is completely dissolved, pay attention to replenishing water during the period, until the liquid turns red completely, and distribute it while it is hot. In the ampoule, each bottle can be filled with 0.5-1 ml as required, and can be sealed in an oxygen-free environment, or placed for 30 days after sealing, to consume the remaining oxygen in the ampoule and reach an anaerobic state.

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Abstract

The invention discloses an anaerobic indicator. The anaerobic indicator consists of the following components in parts by weight: 100-1000 parts of sodium thioglycolate, 10-100 parts of methylene blue, 100-500 parts of a buffering agent, 5-10 parts of phenol red and 100-500 parts of agar. The anaerobic indicator disclosed by the invention has low cost and convenience in use, and is spilled out. Moreover, the color of the anaerobic indicator is easy to judge.

Description

technical field [0001] The invention belongs to the technical field of microorganism cultivation, and can be specifically applied to the anaerobic cultivation of microorganisms or other fields requiring anaerobic environment indication, and is an anaerobic indicator for anaerobic environment indication. Background technique [0002] Anaerobic culture is often used in the process of microbial detection. In anaerobic culture, it is necessary to indicate whether the environment is anaerobic. At present, melanin indicator and resazurin indicator are commonly used in China. Both indicators are liquid indicators. , need to prevent spilling when using, and need to be temporarily prepared, not suitable for storage, the color change range of melanin indicator is from colorless to blue, and the resazurin indicator is from pink to blue, and the transition between light blue and blue Purple has a certain influence on the result judgment. There are tablet indicators abroad, but the colo...

Claims

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Application Information

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IPC IPC(8): C12Q1/04
Inventor 蔡向荣
Owner 青岛高科技工业园海博生物技术有限公司
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