The invention discloses a method for culturing Chlamydomonas reinhardtii by urea plant wastewater. According to the method, urea plant wastewater can be treated in low cost, Chlamydomonas reinhardtii with important economic value can also be obtained, the waste is changed into treasures, harm is turned into a good and many things are achieved at one stroke. A traditional method for culturing Chlamydomonas reinhardtii comprises the step of culturing Chlamydomonas reinhardtii in an open concrete runway pool by virtue of a TAP culture medium, and has the disadvantages of low growth speed, low output, great investment, easy pollution and low efficiency. According to the invention, Chlamydomonas reinhardtii is cultured in a household mineral water bucket to achieve the advantages of low cost, small possibility of pollution, convenience in cleaning, reutilization and convenience in operation; since nutritional ingredients, such as urea plant wastewater, yeast extract powder, sodium deoxycholate, sorbitol, mannitol, sodium thioglycolate, soil extract liquid, sheep manure leachate, chicken manure leachate, L-arginine, potassium dihydrogen phosphate, yeast extract, and diatomite powder are added into the culture medium, and an organic fertilizer is used in combination with an inorganic fertilizer, nutrition is more comprehensive and balanced, the growth rate and yield of Chlamydomonas reinhardtii are substantially increased and the yield is increased by 280%.