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Bacteria culture medium, culture method and application of both

A technology of bacterial culture medium and culture method, applied in the field of strain culture, can solve the problems of low yield and activity, inability to multiply and cultivate at high density, and achieve the effects of high activity, reducing the number of dead bacteria, and promoting a large number of expansions.

Inactive Publication Date: 2018-03-30
浙江同创越诚健康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The first object of the present invention is to provide a kind of bacterial culture medium, the second object of the present invention is to provide a kind of lactic acid bacteria culture method, the 3rd of the present invention is to provide above-mentioned culture medium or culture method in cultivating lactic acid bacteria Application to alleviate the technical problems of low yield and activity per unit of lactic acid bacteria culture in the prior art, and incapable of high-density proliferation and culture

Method used

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Examples

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Effect test

Embodiment 1

[0045] A bacterial culture medium, comprising: 5 parts of glucose, 0.5 part of peptone, 0.1 part of beef extract, 0.1 part of yeast extract, 0.1 part of dipotassium hydrogen phosphate, 0.1 part of triamine citrate, 0.1 part of sodium acetate, and 0.01 part of magnesium sulfate 0.001 part of manganese sulfate, 0.05 part of Tween-80, 0.01 part of L-cysteine ​​and 0.002 part of sodium thioglycolate;

[0046] Make up to 100 parts with purified water.

Embodiment 2

[0048] A bacterial culture medium, comprising: 20 parts of glucose, 2.0 parts of peptone, 1.0 part of beef extract, 1.0 part of yeast extract, 0.5 part of dipotassium hydrogen phosphate, 0.5 part of triamine citrate, 0.5 part of sodium acetate, and 0.08 part of magnesium sulfate part, 0.007 part of manganese sulfate, 0.3 part of Tween-80, 0.03 part of L-cysteine ​​and 0.007 part of sodium thioglycolate;

[0049] Make up to 100 parts with purified water.

Embodiment 3

[0051] A bacterial culture medium, comprising: 10 parts of glucose, 1.0 part of peptone, 0.5 part of beef extract, 0.5 part of yeast extract, 0.2 part of dipotassium hydrogen phosphate, 0.2 part of triamine citrate, 0.2 part of sodium acetate, and 0.05 part of magnesium sulfate part, 0.005 part of manganese sulfate, 0.1 part of Tween-80, 0.02 part of L-cysteine ​​and 0.003 part of sodium thioglycolate;

[0052] Make up to 100 parts with purified water.

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Abstract

The invention relates to the technical field of bacterial strain culture and specifically provides a bacteria culture medium, a culture method and application of both. The bacteria culture medium disclosed by the invention is prepared from a basal culture medium and an additive, wherein the additive is prepared from L-cysteine and sodium thioglycolate. The culture medium can provide a nitrogen source, vitamins, growth factors, fermentable saccharides and the like required by lactic acid bacteria growth and meanwhile can inhibit growth of other infectious microbes; addition of the L-cysteine and sodium thioglycolate additive can slow down dissolution of cell walls of the lactic acid bacteria, so that the amount of dead bacteria in a later fermentation period is reduced, the lactic acid bacteria can keep high-density growth and high activity at the same time, and quick growth and massive proliferation of the lactic acid bacteria are promoted. The lactic acid bacteria culture method disclosed by the invention has the advantages of simpleness, high efficiency, low cost, ability in massively proliferating high-activity and high-density lactic acid bacteria in a short period and significance in industrial culture of the lactic acid bacteria.

Description

technical field [0001] The invention relates to the technical field of bacterial strain cultivation, in particular to a bacterial culture medium, a cultivation method and the application of the two. Background technique [0002] Probiotics, especially lactic acid bacteria, play an important role in maintaining microecological balance and preventing infection. Lactobacillus salivarius is a common lactic acid bacterium in daily life. It has a broad antibacterial spectrum and has a good inhibitory effect on Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Shigella, and Streptococcus pneumoniae. During the growth process, it can secrete substances such as hydrogen peroxide, hydrogen sulfide and antimicrobial peptides, directly inhibit or kill the growth of harmful bacteria; reduce the pH value of the environment by secreting lactic acid and other organic acids, and reduce the growth of pathogenic bacteria; Microorganisms have an important influence, and are widel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/20
CPCC12N1/20C12N1/38
Inventor 徐威李洪涛黄卫新颜洲瑶王波娜张柳莺俞赤慧
Owner 浙江同创越诚健康科技有限公司
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