Bacteria culture medium, culture method and application of both
A technology of bacterial culture medium and culture method, applied in the field of strain culture, can solve the problems of low yield and activity, inability to multiply and cultivate at high density, and achieve the effects of high activity, reducing the number of dead bacteria, and promoting a large number of expansions.
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Embodiment 1
[0045] A bacterial culture medium, comprising: 5 parts of glucose, 0.5 part of peptone, 0.1 part of beef extract, 0.1 part of yeast extract, 0.1 part of dipotassium hydrogen phosphate, 0.1 part of triamine citrate, 0.1 part of sodium acetate, and 0.01 part of magnesium sulfate 0.001 part of manganese sulfate, 0.05 part of Tween-80, 0.01 part of L-cysteine and 0.002 part of sodium thioglycolate;
[0046] Make up to 100 parts with purified water.
Embodiment 2
[0048] A bacterial culture medium, comprising: 20 parts of glucose, 2.0 parts of peptone, 1.0 part of beef extract, 1.0 part of yeast extract, 0.5 part of dipotassium hydrogen phosphate, 0.5 part of triamine citrate, 0.5 part of sodium acetate, and 0.08 part of magnesium sulfate part, 0.007 part of manganese sulfate, 0.3 part of Tween-80, 0.03 part of L-cysteine and 0.007 part of sodium thioglycolate;
[0049] Make up to 100 parts with purified water.
Embodiment 3
[0051] A bacterial culture medium, comprising: 10 parts of glucose, 1.0 part of peptone, 0.5 part of beef extract, 0.5 part of yeast extract, 0.2 part of dipotassium hydrogen phosphate, 0.2 part of triamine citrate, 0.2 part of sodium acetate, and 0.05 part of magnesium sulfate part, 0.005 part of manganese sulfate, 0.1 part of Tween-80, 0.02 part of L-cysteine and 0.003 part of sodium thioglycolate;
[0052] Make up to 100 parts with purified water.
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