Culture medium for separating and counting clostridium butyricum

Abstract

The invention relates to a culture medium for separating and counting clostridium butyricum, and belongs to the field of microbial separation and counting. The culture medium comprises 15.0 g of casein tryptone, 3.0 g of beef extract powder, 1.0 g of yeast extract powder, 5.0 g of sodium chloride, 0.5 g of L-cysteine hydrochloride, 0.5 g of sodium thioglycolate, 10.0 g of xylose, 0.04 g of bromothymol blue, 15.0 g of agar powder, 900ml of purified water, 0.25 g of D-cycloserine, 0.012 g of kanamycin sulfate and 25 ml of yolk. Compared with the prior art, the culture medium has the beneficial effects that firstly, the culture medium provided by the invention can obviously distinguish clostridium butyricum from other bacteria through visual inspection by analyzing the specific physiologicalcharacteristics of clostridium butyricum and carrying out formula combination and adjustment in a targeted manner, so that the purposes of separating and counting clostridium butyricum are achieved; and secondly, the effect is remarkable, direct and effective, and the cost is low.

Description

technical field [0001] The invention relates to a culture medium for isolating and counting Clostridium butyricum, belonging to the field of microorganism separation and counting. Background technique [0002] Clostridium butyricum (Clostridium butyricum) belongs to the Bacillus family, Clostridium genus, Gram-positive, with spores, spores oval, eccentric or subterminal, and resistant to adverse environments. [0003] Clostridium butyricum is used as a feed additive in feed, and it can embody five biological characteristics in the intestinal tract of animals: First, it promotes the proliferation and development of beneficial bacteria in the intestinal tract of animals (Bifidobacteria, Lactobacillus), inhibits harmful bacteria and The growth and reproduction of spoilage bacteria can correct the disorder of intestinal flora and reduce the occurrence of enterotoxin; the second is that it can produce B vitamins, vitamin K, amylase and other substances in the intestinal tract of ...

AI Technical Summary

Problems solved by technology

Clostridium butyricum often coexists with other bacteria such as Escherichia coli, Clostridium perfringens, and lactic acid bacteria in the above-mentioned fields, so it is necessary to realize the quantitative analysis of Clostridium butyricum. The detection method of bacteria is mainly based on the culture medium of Clostridium perfringens, but the above detection method has shortcomings. Consistency, so a series of tedious biochemical identification or 16SrRNA homology comparison is required in the later stage, which is hardly operable for enterprise inspectors, so there must be a method on the market that can compare Clostridium butyricum with other bacteria Ways to distinguish effectively

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