Culture medium of anaerobic toxin production in use for producing vaccine of treating disease of sheep clostridium

Abstract

An anaerobic toxigenic culture medium for preparing the vaccine of clostridial disease of sheep is prepared from peptone, powder extract of yeast, NaCl, Na2HPO4.12H2O, coarse dextrin, sodium thioalcoholate, glucose, ZnSo4.7H2O, sodium sulfite and water.

Description

1. Technical field [0001] The invention relates to a microorganism culture medium, in particular to an anaerobic toxin-producing culture medium for the production of a clostridioides disease vaccine. 2. Background technology [0002] Clostridial disease is a class of infectious diseases of various animals caused by a variety of Clostridium in the genus Clostridium anaerobes, such as rapid plague, sudden attack, dysentery, enterotoxemia, emphysema, black plague, botulism Clostridium poisoning, tetanus, etc. This type of disease is characterized by rapid onset, short course of disease, lack of treatment and high mortality. Most of the pathogenic bacteria are soil bacteria, which widely exist all over the world and cause great economic losses. The immunity of Clostridial diseases is mainly achieved by inactivating the toxin produced during the culture of Clostridia, which is the main effective immune antigen of the vaccine. Therefore, by cultivating such bacteria, allowing t...

AI Technical Summary

Problems solved by technology

On the other hand, it is prepared with a large amount of beef, liver, pepsin and other animal-derived ingredients. Digest at 53--55°C for 24 hours (continuous stirring is required during this period), and finally, add other ingredients to heat, filter and other procedures to make

It takes 6 people and 3 days to prepare a batch of culture medium for production, which is costly and poor in economic benefits

At the same time, it will also be affected by the quality of the meat (such as antibiotic residues, etc.), making the quality of the medium unstable and unsuitable for quality control.