Culture medium of anaerobic toxin production in use for producing vaccine of treating disease of sheep clostridium

A technology for production of toxin-producing medium and vaccine, which is applied to bacteria, medical raw materials derived from bacteria, etc., can solve the problems of high cost, poor economic benefit, complicated process, etc., and achieve the effects of stable quality and low cost.

Inactive Publication Date: 2005-11-16
新疆维吾尔自治区畜牧科学院兽医研究所
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Problems solved by technology

On the other hand, it is prepared with a large amount of beef, liver, pepsin and other animal-derived ingredients. Digest at 53--55°C for 24 hours (continuous stirring is required during this period), and finally, add other ingredients to heat, filter and other procedures to make
It takes 6 p...

Method used

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Embodiment Construction

[0039] Produce 1000ml substratum of the present invention to choose

[0040] Peptone 20g Yeast extract powder 3g

[0041] NaCl 2g Na 2 HPO4·12H 2 O 8g

[0042] Crude dextrin 8g Sodium thioglycolate 0.05g

[0043] Glucose 5g ZnSO 4 ·7H 2 O 0.04g

[0044] Sodium sulfite 0.2g

[0045] The rest is distilled water

[0046] The production method is: mix peptone, yeast extract powder, NaCl, Na 2 HPO4·12H 2 O, crude dextrin, sodium thioglycolate, glucose, sodium sulfite, and ZnSO 4 ·7H 2 O, add an appropriate amount of distilled water to mix and dissolve in sequence, and sterilize at 121°C for 30 minutes to obtain the medium of the present invention.

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Abstract

An anaerobic toxigenic culture medium for preparing the vaccine of clostridial disease of sheep is prepared from peptone, powder extract of yeast, NaCl, Na2HPO4.12H2O, coarse dextrin, sodium thioalcoholate, glucose, ZnSo4.7H2O, sodium sulfite and water.

Description

1. Technical field [0001] The invention relates to a microorganism culture medium, in particular to an anaerobic toxin-producing culture medium for the production of a clostridioides disease vaccine. 2. Background technology [0002] Clostridial disease is a class of infectious diseases of various animals caused by a variety of Clostridium in the genus Clostridium anaerobes, such as rapid plague, sudden attack, dysentery, enterotoxemia, emphysema, black plague, botulism Clostridium poisoning, tetanus, etc. This type of disease is characterized by rapid onset, short course of disease, lack of treatment and high mortality. Most of the pathogenic bacteria are soil bacteria, which widely exist all over the world and cause great economic losses. The immunity of Clostridial diseases is mainly achieved by inactivating the toxin produced during the culture of Clostridia, which is the main effective immune antigen of the vaccine. Therefore, by cultivating such bacteria, allowing t...

Claims

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Application Information

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IPC IPC(8): C12N1/20
Inventor 黄炯张读朴符子华
Owner 新疆维吾尔自治区畜牧科学院兽医研究所
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