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A kind of anaerobic growth medium and preparation method thereof

A technology of enrichment medium and configuration method, applied in the field of anaerobic enrichment medium and its preparation, can solve the problems of long result time, inability to provide timely clinical guidance, slow growth rate, etc. fertilization rate, and the effect of promoting reproduction

Active Publication Date: 2016-10-26
SHANDONG XINKE BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the harsh requirements of anaerobic bacteria on environmental conditions, the growth rate is slow, and the laboratory report results take a long time, which cannot provide timely clinical guidance

Method used

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  • A kind of anaerobic growth medium and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0031] An anaerobic enrichment medium comprising: 25 grams of peptone, 2 grams of yeast extract powder, 1 gram of brain heart infusion broth, 2 grams of glucose, 3 grams of sodium thioglycolate, 0.005 grams of hemin, Vitamin K 1 0.0005 g, 0.35 g of anticoagulant, 0.0001 g of anaerobic indicator, 1000 ml of distilled water, 0.1 g of L-cysteine, and 0.4 g of sodium bicarbonate.

[0032] In this embodiment, the peptone is tryptone; the anticoagulant is sodium polyanethole sulfonate; and the anaerobic indicator is resazurin.

[0033] The configuration method of above-mentioned anaerobic enrichment medium comprises the following steps:

[0034] (1) Prepare raw materials: 25 grams of peptone, 2 grams of yeast extract powder, 1 gram of brain heart infusion broth, 2 grams of glucose, 3 grams of sodium thioglycolate, 0.005 grams of hemin, 0.35 grams of anticoagulant, Anaerobic indicator 0.0001 g, L-cysteine ​​0.1 g, sodium bicarbonate 0.4 g.

[0035] (2) Put the raw materials in ste...

Embodiment 2

[0049] An anaerobic enrichment medium comprising: 20 grams of peptone, 1.5 grams of yeast extract powder, 0.5 grams of brain heart infusion broth, 0.6 grams of glucose, 2.5 grams of sodium thioglycolate, 0.003 grams of hemin, Vitamin K 1 0.0003 g, 0.3 g of anticoagulant, 0.00005 g of anaerobic indicator, 900 ml of distilled water, 0.05 g of L-cysteine, and 0.3 g of sodium bicarbonate.

[0050] In this embodiment, the peptone is soybean peptone; the anticoagulant is p-aminobenzoic acid; and the anaerobic indicator is methylene blue (methylene blue).

[0051] The configuration method of above-mentioned anaerobic enrichment medium comprises the following steps:

[0052] (1) Prepare raw materials: 20 grams of peptone, 2.5 grams of yeast extract powder, 0.5 grams of brain heart infusion broth, 0.6 grams of glucose, 2.5 grams of sodium thioglycolate, 0.003 grams of hemin, 0.3 grams of anticoagulant, Anaerobic indicator 0.00005 grams, L-cysteine ​​0.05 grams, sodium bicarbonate 0.3...

Embodiment 3

[0060] An anaerobic enrichment medium comprising: 28 grams of peptone, 4 grams of yeast extract powder, 1.5 grams of brain heart infusion broth, 2.5 grams of glucose, 4 grams of sodium thioglycolate, 0.008 grams of hemin, Vitamin K 1 0.0008 g, 0.5 g of anticoagulant, 0.0003 g of anaerobic indicator, 1100 ml of distilled water, 0.15 g of L-cysteine, and 0.6 g of calcium bicarbonate.

[0061] In this embodiment, the peptone is polyvalent peptone; the anticoagulant is magnesium sulfate; and the anaerobic indicator is resazurin.

[0062] The configuration method of above-mentioned anaerobic enrichment medium comprises the following steps:

[0063] (1) Prepare raw materials: 28 grams of peptone, 4 grams of yeast extract powder, 1.5 grams of brain heart infusion broth, 2.5 grams of glucose, 4 grams of sodium thioglycolate, 0.008 grams of hemin, 0.5 grams of anticoagulant, Anaerobic indicator 0.0003 grams, L-cysteine ​​0.15 grams, calcium bicarbonate 0.6 grams.

[0064] (2) Put th...

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Abstract

The invention belongs to the technical field of microbial culture, and concretely relates to an anaerobic enrichment medium and a preparation method thereof. The medium includes peptone, yeast extract powder, a brain and heart infusion broth, glucose, sodium thioglycolate, hemin, vitamin K1, an anticoagulant, an anaerobic indicator and distilled water; and the medium for promoting mass propagation of anaerobic bacteria can be prepared through a special ratio of the above components. The medium is used to clinically culture anaerobic pathogens in order to improve the positive detection rate of the anaerobic pathogens.

Description

technical field [0001] The invention belongs to the technical field of microorganism cultivation, and in particular relates to an anaerobic bacteria enrichment medium and a preparation method thereof. Background technique [0002] Bacteremia caused by anaerobic bacteria has been on the rise in recent years and causes a high mortality rate. In the 1970s, the isolation rate of anaerobic bacteria in blood culture was as high as 20% to 30%, of which Bacteroides fragilis accounted for 78%, second only to Escherichia coli among Gram-negative bacteria. In recent years, the emergence of drug resistance of anaerobic bacteria has challenged empirical treatment, and the clinical infection and clinical consequences caused by anaerobic bacteria have received renewed attention. Anaerobic blood culture is the gold standard method for rapid and accurate diagnosis of anaerobic bacteremia. [0003] Retrospective studies on anaerobic bacteremia show that the mortality rate of anaerobic bacte...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/04
Inventor 张敏王娜杨伟伟
Owner SHANDONG XINKE BIOLOGICAL TECH
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