Medium for the isolation and enumeration of Clostridium butyricum

Abstract

The invention relates to a culture medium for isolating and counting Clostridium butyricum, belonging to the field of microorganism separation and counting. Including tryptone 15.0g, beef extract powder 3.0g, yeast extract powder 1.0g, sodium chloride 5.0g, L-cysteine ​​hydrochloride 0.5g, sodium thioglycolate 0.5g, xylose 10.0g, Bromothymol blue 0.04g, agar powder 15.0g, purified water 900ml, D-cycloserine 0.25g, kanamycin sulfate 0.012g, egg yolk 25ml. Compared with the prior art, the beneficial effects of the present invention are: first, the culture medium of the present invention can make Clostridium butyricum It can be clearly distinguished from other bacteria by visual observation, thereby achieving the purpose of isolating and counting Clostridium butyricum. Second, the effect is remarkable, direct and effective, and low in cost.

Description

Technical field [0001] The invention relates to a culture medium for separating and counting Clostridium butyricum, belonging to the field of microorganism separation and counting. Background technique [0002] Clostridium butyricum (Clostridium butyricum) belongs to the Bacillus family, Clostridium, Gram-positive, spores, spore oval, eccentric or subterminal, and can resist bad environment. [0003] Clostridium butyricum is used as a feed additive in feed, and it can reflect five major biological characteristics in the animal intestine: one is to promote the proliferation and development of the animal intestinal beneficial flora (Bifidobacterium, Lactobacillus), and inhibit harmful bacteria and The growth and reproduction of spoilage bacteria can correct the disorder of intestinal flora and reduce the occurrence of intestinal toxins; the second is that it can produce B vitamins, vitamin K, amylase and other substances in the animal intestine, which has a health care effect; the th...

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Problems solved by technology

Clostridium butyricum often coexists with other bacteria such as Escherichia coli, Clostridium perfringens, and lactic acid bacteria in the above-mentioned fields, so it is necessary to realize the quantitative analysis of Clostridium butyricum. The detection method of bacteria is mainly based on the culture medium of Clostridium perfringens, but the above detection method has shortcomings. Consistency, so a series of tedious biochemical identification or 16SrRNA homology comparison is required in the later stage, which is hardly operable for enterprise inspectors, so there must be a method on the market that can compare Clostridium butyricum with other bacteria Ways to distinguish effectively

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