Medium for the isolation and enumeration of Clostridium butyricum

Abstract

The invention relates to a culture medium for isolating and counting Clostridium butyricum, belonging to the field of microorganism separation and counting. Including tryptone 15.0g, beef extract powder 3.0g, yeast extract powder 1.0g, sodium chloride 5.0g, L-cysteine ​​hydrochloride 0.5g, sodium thioglycolate 0.5g, xylose 10.0g, Bromothymol blue 0.04g, agar powder 15.0g, purified water 900ml, D-cycloserine 0.25g, kanamycin sulfate 0.012g, egg yolk 25ml. Compared with the prior art, the beneficial effects of the present invention are: first, the culture medium of the present invention can make Clostridium butyricum It can be clearly distinguished from other bacteria by visual observation, thereby achieving the purpose of isolating and counting Clostridium butyricum. Second, the effect is remarkable, direct and effective, and low in cost.

Description

Technical field

[0001] The invention relates to a culture medium for separating and counting Clostridium butyricum, belonging to the field of microorganism separation and counting. Background technique

[0002] Clostridium butyricum (Clostridium butyricum) belongs to the Bacillus family, Clostridium, Gram-positive, spores, spore oval, eccentric or subterminal, and can resist bad environment.

[0003] Clostridium butyricum is used as a feed additive in feed, and it can reflect five major biological characteristics in the animal intestine: one is to promote the proliferation and development of the animal intestinal beneficial flora (Bifidobacterium, Lactobacillus), and inhibit harmful bacteria and The growth and reproduction of spoilage bacteria can correct the disorder of intestinal flora and reduce the occurrence of intestinal toxins; the second is that it can produce B vitamins, vitamin K, amylase and other substances in the animal intestine, which has a health care effect; the th...

Examples

Embodiment Construction

[0017] The principles and features of the present invention will be described below, and the examples given are only used to explain the present invention, not to limit the scope of the present invention.

[0018] A medium for separating and counting Clostridium butyricum, including 15.0g tryptone casein, 3.0g beef extract powder, 1.0g yeast extract powder, 5.0g sodium chloride, and 0.5g L-cysteine ​​hydrochloride , Sodium thioglycolate 0.5g, xylose 10.0g, bromothymol blue 0.04g, agar powder 15.0g, purified water 900ml, D-cycloserine 0.25g, kanamycin sulfate 0.012g, cefoxitin 0.03g , Polymyxin B sulfate 100,000 U, neomycin sulfate 0.1g, egg yolk 25ml, adding a specific carbon source (xylose) to metabolize Clostridium butyricum to produce acid, which causes the pH of the medium to change and present a special color. The effective combination of antibiotics and antibacterial agents (D-cycloserine) can inhibit the growth of most Clostridium butyricum except for Clostridium butyricum...

Technical field

[0001] The invention relates to a culture medium for separating and counting Clostridium butyricum, belonging to the field of microorganism separation and counting. Background technique

[0002] Clostridium butyricum (Clostridium butyricum) belongs to the Bacillus family, Clostridium, Gram-positive, spores, spore oval, eccentric or subterminal, and can resist bad environment.

[0003] Clostridium butyricum is used as a feed additive in feed, and it can reflect five major biological characteristics in the animal intestine: one is to promote the proliferation and development of the animal intestinal beneficial flora (Bifidobacterium, Lactobacillus), and inhibit harmful bacteria and The growth and reproduction of spoilage bacteria can correct the disorder of intestinal flora and reduce the occurrence of intestinal toxins; the second is that it can produce B vitamins, vitamin K, amylase and other substances in the animal intestine, which has a health care effect; the th...

Examples

Embodiment Construction

[0017] The principles and features of the present invention will be described below, and the examples given are only used to explain the present invention, not to limit the scope of the present invention.

[0018] A medium for separating and counting Clostridium butyricum, including 15.0g tryptone casein, 3.0g beef extract powder, 1.0g yeast extract powder, 5.0g sodium chloride, and 0.5g L-cysteine ​​hydrochloride , Sodium thioglycolate 0.5g, xylose 10.0g, bromothymol blue 0.04g, agar powder 15.0g, purified water 900ml, D-cycloserine 0.25g, kanamycin sulfate 0.012g, cefoxitin 0.03g , Polymyxin B sulfate 100,000 U, neomycin sulfate 0.1g, egg yolk 25ml, adding a specific carbon source (xylose) to metabolize Clostridium butyricum to produce acid, which causes the pH of the medium to change and present a special color. The effective combination of antibiotics and antibacterial agents (D-cycloserine) can inhibit the growth of most Clostridium butyricum except for Clostridium butyricum...

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