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Group of microsatellite primers for diversity analysis of early blight pathogen alternaria solani

A technology of Alternaria solani, diversity analysis, applied in the direction of recombinant DNA technology, microbial measurement/testing, biochemical equipment and methods, etc.

Inactive Publication Date: 2014-12-10
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
  • Description
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Problems solved by technology

So far, there is no set of microsatellite primers for analyzing the genetic structure of Alternaria solani in the world

Method used

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  • Group of microsatellite primers for diversity analysis of early blight pathogen alternaria solani
  • Group of microsatellite primers for diversity analysis of early blight pathogen alternaria solani
  • Group of microsatellite primers for diversity analysis of early blight pathogen alternaria solani

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Embodiment 1

[0012] 1. Primer synthesis: polymorphic-rich microsatellite primers were screened from the genome library of the early blight pathogen Alternaria solani, and 7 pairs of primers were synthesized using a DNA synthesizer. The sequence is as follows:

[0013]

[0014] 2. Extraction of total genome DNA: "Plant Genome Total DNA Rapid Extraction Kit" purchased from BIOMIGA Company, product number GD2611: refer to the product manual for specific operations.

[0015] 1) Alternaria solani strains were cultured on fresh medium, and about 100 mg of mycelia were collected.

[0016] 2) Take powdery dry tissue, put it in a mortar, add 950 μL Buffer P1, and grind at room temperature until there is no obvious suspended particles.

[0017] 3) Transfer all the ground mixture to a 2mL centrifuge tube, add 10μL of β-mercaptoethanol, vortex briefly, place in a water bath at 65°C for 10min, and invert and mix several times during the process (if RNA needs to be removed,...

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Abstract

The invention discloses a group of microsatellite primers for diversity analysis of early blight pathogen alternaria solani, and provides a method for performing diversity and population genetic structure analysis by using the group of primers. The group of primers is screened and designed from a genome library. The group of primers is stable in PCR (polymerase chain reaction) amplification products and favorable in polymorphism, and can be used for diversity and population genetic structure analysis.

Description

technical field [0001] The invention belongs to the field of plant protection, and in particular relates to a group of microsatellite primers for analyzing the diversity of the early blight pathogen Alternaria solani, which can be applied to the genetic diversity analysis of the potato early blight pathogen Alternaria solani population. Background technique [0002] Potato is the fourth largest food crop in the world after rice, wheat and corn. Potato early blight caused by Alternaria solani Sorauer occurs in potato growing areas all over the world. Early blight can repeatedly infect potatoes during the growing period, and overwinter in potato blocks or soil in the form of conidia. At present, there is a more and more serious trend in some areas of our country, which has caused huge losses to local potato production. [0003] Microsatellite DNA, also known as SSR (Simple sequence repeat), is a simple tandem repeat sequence composed of several bases (generally 1-6) as the re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12Q1/04C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 祝雯蒙静雯詹家绥
Owner FUJIAN AGRI & FORESTRY UNIV
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