Compound collecting method based on low-pH-value capillary zone electrophresis

A technology of capillary electrophoresis and zone electrophoresis, which is applied in separation methods, chemical instruments and methods, and separation of dispersed particles. The effect of concentration and inhibition of adsorption
CN103551035AInactive Publication Date: 2014-02-05BEIJING INSTITUTE OF TECHNOLOGYGY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BEIJING INSTITUTE OF TECHNOLOGYGY
Publication Date
2014-02-05
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a compound collecting method based on low-pH-value capillary zone electrophresis, and belongs to the technical field of bio-separation and analysis. The method comprises the steps of incubating protein and a fluorescence labeling ssDNA (single-stranded DNA) library on ice after mixing; separating an incubated mixed sample by low-pH-value capillary zone electrophresis, wherein the pH value of electrophresis running buffer liquid is less than 3; in electrophresis separation, moving dissociated protein, dissociated ssDNA and a compound formed by the protein and the ssDNA in the mixed sample in the direction opposite to the charge characteristic according to charge characteristics of the dissociated protein, dissociated ssDNA and the compound formed by protein and ssDNA respectively; carrying out CE-LIF (capillary electrophresis laser induced fluorescence detector) analysis on collection collected at the outlet end and the inlet end of a capillary, and testing the method to be feasible. The method can inhibit electroosmotic flow and inhibit the adsorption of positive charge samples and alkaline protein on the inner wall of the capillary; the online separation of samples with different charge types is realized; the sample online enrichment amount can be increased through increasing the sample feed amount; more protein and ssDNA compounds can be obtained through analysis once.
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Description

technical field

[0001] The invention relates to a method for collecting complexes based on low-pH capillary zone electrophoresis. The complexes are protein and ssDNA complexes, and belong to the technical field of biological separation and analysis. Background technique

[0002] The oligonucleotide library is a class containing 10 13 ~10 15 A mixture of single-stranded DNA molecules (single-stranded DNA, ssDNA) with different bases, which may contain one or several DNA molecules that bind to target molecules with high specificity and high affinity. Nucleic acid aptamer (Aptamer) refers to the oligonucleotide with high affinity and high specificity to the target molecule, which is screened from the random oligonucleotide library through the exponential enrichment ligand system evolution (SELEX) technology Acid sequence ligands. Nucleic acid aptamers have high affinity, strong specificity, easy preparation and modification, and wide distribution of target molecules. [000...

Claims

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