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Haematococcus pluvialis culture medium

A technology of Haematococcus pluvialis and culture medium, which is applied in the directions of microorganisms, unicellular algae, microorganism-based methods, etc., can solve the problem that there is no research on the effect of organic phosphorus sources on the growth of Haematococcus, and there is no regulation of Haematococcus cells. growth and other issues, to shorten the growth cycle, improve the probability of success, and increase yield.

Active Publication Date: 2014-02-19
QINGDAO XUNON BIOLOGICAL ENG
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0007] But so far, there is no research on the effect of organic phosphorus sources on the growth of Haematococcus, and there is no discussion on the regulation of cell growth of Haematococcus by combining inorganic phosphorus sources and organic phosphorus sources

Method used

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  • Haematococcus pluvialis culture medium
  • Haematococcus pluvialis culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) Each liter of medium contains

[0043] NaNO 3 0.32 g, KNO 3 0.17 g, MgSO 4 ·7H 2 O 0.07g, Na 2 SO 3 0.032g, C 6 h 5 Na3 o 7 0.11 g, KH 2 PO 4 0.02 g, G-P 0.015 g, CaCl 2 0.03 g, EDTANaFe 0.0002 g, FeSO 4 0.001 g, ZnSO 4 0.0015 g, MnCl 2 0.00006 g, CoCl 2 0.00003 g, H 3 BO 3 0.0001 g, CuSO 4 0.00006 g, (NH 4 )Mo 7 o 24 0.00002 g, 3-IBA 0.002 g, 6-BA 0.0003 g, Vb 12 0.000025~0.00005 g.

[0044] (2) Add 100 mL of the medium shown above in a 250 mL Erlenmeyer flask, the inoculation ratio is 10% (that is, the volume of the seed liquid accounts for 10% of the culture volume), and the seeds are Haematococcus strain I in the logarithmic growth phase , the inoculation density was 10 3 ~10 4 cells / mL. The culture temperature was (23 ± 1.5) °C, the culture light intensity was 1300-1500 lx, and the culture room was continuously illuminated for static culture. During this period, the culture was shaken several times a day, and three replica...

Embodiment 2

[0052] (1) Each liter of medium contains

[0053] NaNO 3 0.35 g, KNO 3 0.15 g, MgSO 4 ·7H 2 O 0.14 g, Na 2 SO 3 0.05 g, C 6 h 5 Na 3 o 7 0.20 g, KH 2 PO 4 0.02 g, G-P 0.010 g, CaCl 2 0.06 g, EDTANaFe 0.00025 g, FeSO 4 0.002 g, ZnSO 4 0.0030 g, MnCl 2 0.00012 g, CoCl 2 0.00007 g, H 3 BO 3 0.0002 g, CuSO 4 0.00012 g, (NH 4 )Mo 7 o 24 0.00004 g, 3-IBA 0.002 g, 6-BA 0.0003 g, Vb 12 0.000025~0.00005 g.

[0054] (2) Add 100 mL of the medium shown above in a 250 mL Erlenmeyer flask, the inoculation ratio is 10% (that is, the volume of the seed liquid accounts for 10% of the culture volume), and the seeds are Haematococcus strain I in the logarithmic growth phase , the inoculation density was 10 3 ~10 4 cells / mL. The culture temperature was (23 ± 1.5) °C, the culture light intensity was 1300-1500 lx, and the culture room was continuously illuminated for static culture. During this period, the culture was shaken several times a day, and three rep...

Embodiment 3

[0061] (1) Each liter of medium contains

[0062] NaNO 3 0.34 g, KNO 3 0.16 g, MgSO 4 ·7H 2 O 0.13 g, Na 2 SO 3 0.045 g, C 6 h 5 Na 3 o 7 0.22 g, KH 2 PO 4 0.02 g, G-P 0.02 g, CaCl 2 0.07 g, EDTANaFe 0.00024 g, FeSO 4 0.0018 g, ZnSO 4 0.0024 g, MnCl 2 0.00015 g, CoCl 2 0.00005 g, H 3 BO 3 0.00019 g, CuSO 4 0.00014 g, (NH 4 )Mo 7 o 24 0.00003 g, 3-IBA 0.002 g, 6-BA 0.0003 g, Vb 12 0.000025~0.00005 g.

[0063] (2) Add 100 mL of the medium shown above in a 250 mL Erlenmeyer flask, the inoculation ratio is 10% (that is, the volume of the seed liquid accounts for 10% of the culture volume), and the seeds are Haematococcus strain I in the logarithmic growth phase , the inoculation density was 10 3 ~10 4 cells / mL. The culture temperature was (23 ± 1.5) °C, the culture light intensity was 1300-1500 lx, and the culture room was continuously illuminated for static culture. During this period, the culture was shaken several times a day, and three r...

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Abstract

The invention provides a haematococcus pluvialis culture medium. The culture medium per liter contains 0.30-0.40g of NaNO3, 0.10-0.20g of KNO3, 0.07-0.18g of MgSO4.7H2O, 0.03-0.06g of Na2SO3, 0.10-0.30g of C6H5Na3O7, 0.02g of inorganic phosphorus, 0.005-0.02g of organic phosphorus, 0.03-0.09g of CaC12, 0.0002-0.0003g of EDTA-NaFe, 0.001-0.003g of FeSO4, 0.0015-0.0045g of ZnSO4, 0.00006-0.00018g of MnCl2, 0.00003-0.0001g of CoCl2, 0.0001-0.0003g of H3BO3, 0.00006-0.00018g of CuSO4, 0.00002-0.00006g of (NH4) Mo7O24, 0.002g of 3-IBA, 0.0003g of 6-BA and 0.000025-0.00005g of Vb12. The culture medium is used for photoautotrophically culturing haematococcus pluvialis, can significantly promote the growth of haematococcus pluvialis, enables the haematococcus pluvialis to rapidly enter the exponential growth phase so as to achieve higher growth density and keep the dominant position of the population of haematococcus pluvialis in a short time, and therefore is conducive to furthest reducing pollution probabilities of foreign bacteria, seaweeds and protozoa, shortening the growth cycle and increasing the successful probability of culture in the field enlarging culture process.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a culture medium for Haematococcus pluvialis. Background technique [0002] Astaxanthin is a fat-soluble carotenoid with wide application value and development prospects. It has two characteristics: one is that it has strong biological activity and physiological function, and it is the strongest antioxidant known in nature. Its antioxidant capacity is 10 times that of β-carotene and 100-550 times that of vitamin E. It is known as "super vitamin E". Second, it is bright red in color and has a strong coloring ability. Studies have shown that astaxanthin has a powerful ability to scavenge free radicals and resist oxidation, and at the same time has obvious effects of protecting blood vessels, improving retinal, preventing light damage, anti-aging, anti-cancer and enhancing body immunity. Therefore, astaxanthin has broad application prospects in the fields of health care products, pharm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 陈伟徐瑶柴文波潘倩倩干松浩刘永梅
Owner QINGDAO XUNON BIOLOGICAL ENG
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