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Method for detecting microcystin-LR by immunochromatographic test strip

The technology of immunochromatographic test paper and microcystin is applied in the field of immunoanalytical chemistry, which can solve the problems of difficult preservation of gold nanoparticle sol, and achieve the effects of overcoming difficulty in preservation, short time and simple operation.

Active Publication Date: 2014-02-26
YUNNAN NORMAL UNIV
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Problems solved by technology

[0008] The purpose of the present invention is to overcome the shortcoming that currently commonly used colored marker gold nanoparticle sol is not easy to preserve, and to provide a method for detecting microcystin-LR with an immunochromatographic test strip, which is simple to operate and requires less time for antibody modification. short

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  • Method for detecting microcystin-LR by immunochromatographic test strip
  • Method for detecting microcystin-LR by immunochromatographic test strip
  • Method for detecting microcystin-LR by immunochromatographic test strip

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Embodiment Construction

[0043] See Figure 4 ~ Figure 7 the embodiment.

[0044] 1. Preparation of gold nanocage Au NCs

[0045] The gold nanocage involved in this example is prepared by the following method: add 3 mL of 0.75 mmol / L HAuCl to the small Erlenmeyer flask 4 solution, and then add 3 mL of 0.03 mol / L HMT solution, shake well. When the solution gradually became lighter from bright yellow to clear, add 3 mL of 0.30 mol / L PVP and 100 μL of 0.01 mol / L AgNO 3 solution. After shaking gently, quickly add 50 μL of 0.08 mol / L ascorbic acid solution. Stir rapidly for 10 s to ensure that the solution is well mixed. The vial was placed in a dark place at room temperature for 12 hours, and then the product was centrifuged and washed three to five times with water and ethanol. The obtained product was stored at 4°C protected from light. The transmission electron microscope figure of embodiment gold nanocage sees Figure 5 .

[0046] 2. Preparation and purification of gold nanocage-labeled monoc...

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Abstract

The invention relates to a method for detecting microcystin-LR by an immunochromatographic test strip and belongs to the technical field of immunoassay chemistry. The method comprises the following steps: preparing Au NCs with a hollow structure by a one-step preparation method, marking Au NCs on Anti MC-LR to prepare a gold nanocage-microcystin antibody conjugate, and respectively spraying BSA (bovine serum albumin)-coupled microcystin-LR and IgG to a nitrocellulose membrane for being used as a detection line and a control line to prepare an immunochromatographic strip. The microcystin-LR in a test sample is analyzed in a quantitative manner by reading the gray-scale value of the gold nanocage-microcystin antibody conjugate which is retained on the detection line through a competitive immunochromatographic method. The shortcoming that gold sol is not easy to preserve in the technology of the test strips can be overcome; furthermore, the operation is simple, and the time required for modifying an antibody is relatively short.

Description

[0001] technical field [0002] The invention belongs to the technical field of immunoanalysis chemistry. Background technique [0003] Human life and industrial activities discharge more and more sewage into lakes and other water bodies, resulting in eutrophication of water bodies, abnormal proliferation of algae, and release of secondary metabolites algal toxins, especially microcystins, which seriously endanger the natural ecological environment. There is an urgent need to develop simple and efficient detection methods for microcystins in the environmental protection of water bodies. [0004] Immunochromatography as a new technology can be traced back to the 1950s. In 1957, Kohn reported a new immunoelectrophoresis technology, the initial form of immunochromatography technology, and tried to use this technology to separate a known protein from an unknown protein in 1958, and obtained a separation better than agar diffusion electrophoresis. method. The basic principle...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
CPCG01N33/532G01N33/558B01D61/00
Inventor 杨云慧牛司朋赵锦航符雪文赵春玲
Owner YUNNAN NORMAL UNIV
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