Kit for detecting active tuberculosis based on antigen-specific TNF-alpha-ELISA (enzyme linked immunosorbent assay) and application thereof

A kit and specific technology, applied in biological testing, measuring devices, disease diagnosis, etc., can solve the problems of similar products that have not been developed for the diagnosis of active tuberculosis
CN103604933AInactive Publication Date: 2014-02-26TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
Publication Date
2014-02-26
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a kit for detecting active tuberculosis based on antigen-specific TNF-alpha-ELISA (enzyme linked immunosorbent assay) and an application thereof. The kit comprises an ELISA plate coating a TNF-alpha antibody, a TNF-alpha protein standard, protein liquid of ESAT-6 and CFP-10, a biotin-marked TNF-alpha antibody and avidin-marked horseradish peroxidase. By adopting the kit for detecting active tuberculosis based on TNF-alpha-ELISA, a method for distinguishing active tuberculosis infection from latent tuberculosis infection is established; the TNF-alpha released by the peripheral blood mononuclear cell under the stimulus of tuberculosis-specific antigens ESAT-6 and CFP-10 and the background TNF-alpha released by the peripheral blood mononuclear cell without stimulus are quantitatively detected through the ELISA technology, and the value of the antigen-specific TNF-alpha is obtained by subtracting the two so as to perform direct diagnosis on the active tuberculosis.
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Description

technical field

[0001] The invention relates to the application field of ELISA kits, in particular to a kit for detecting active tuberculosis based on antigen-specific TNF-α-ELISA and its application. Background technique

[0002] Tuberculosis is one of the major diseases that threaten human health. It mainly causes lung diseases, and its morbidity and mortality are high. In history, tuberculosis was widely prevalent in the world, killing hundreds of millions of people. Since the 1950s, anti-tuberculosis drugs have been discovered continuously, and the prevalence of tuberculosis has been controlled to a certain extent. In recent years, due to the neglect of tuberculosis prevention and control work in some countries, coupled with factors such as the increase of floating population, the spread of HIV-infected people and the continuous emergence of drug-resistant tuberculosis strains, the prevalence of tuberculosis has rebounded. At present, there are 8 million new cases ever...

Claims

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