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Method for preparing bacillus amyloliquefaciens Sh1 microcapsule

A technology of amylolytic spores and microcapsules, which is applied in the field of microbial additives for aquaculture feed, and can solve the problem of low effective bacteria content

Inactive Publication Date: 2014-03-12
SHANGHAI OCEAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the effective bacterial content of microcapsules prepared by spray drying technology is generally not high. For Bacillus amyloliquefaciens Sh1 microcapsules, the effective bacterial content has become a key factor for large-scale industrial applications.

Method used

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  • Method for preparing bacillus amyloliquefaciens Sh1 microcapsule

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Aseptically take 1mL of Bacillus amyloliquefaciens Sh1 suspension and add it to sterile gelatin solutions with concentrations of 2%, 3%, 4%, 5%, and 6%, respectively, and then stir on a magnetic stirrer at 30°C and 100r / min. Mix thoroughly and evenly, and spray dry under the conditions of air inlet temperature 130°C, feed rate 10ml / min, air flow 700L / h, needle pass frequency 10s, etc., respectively for Bacillus amyloliquefaciens Sh1 prepared under different gelatin solution concentrations Microcapsules were used to determine the effective bacterial content.

Embodiment 2

[0047] Aseptically take 1mL of the suspension of Bacillus amyloliquefaciens Sh1 and add it to the sterile gelatin solution with a concentration of 3%, then fully mix it evenly on a magnetic stirrer at 30°C and 100r / min, and mix it evenly at the inlet air temperature of 110 ℃, 120°C, 130°C, 140°C, 150°C, 160°C, 170°C, and the feed rate is 10ml / min, the air flow rate is 700L / h, and the needle frequency is 10s. The Bacillus amyloliquefaciens Sh1 microcapsules prepared below were used for the determination of the effective bacterial content.

Embodiment 3

[0049] Aseptically take 1mL of the suspension of Bacillus amyloliquefaciens Sh1 and add it to the sterile gelatin solution with a concentration of 3%, then fully mix evenly on a magnetic stirrer at 30°C and 100r / min, and feed at a feeding speed of 6 , 8, 10, 12, 14ml / min, air inlet temperature 150°C, air flow rate 700L / h, needle pass frequency 10s and other conditions for spray drying, the Bacillus amyloliquefaciens Sh1 microcapsules prepared at different feed rates Determination of effective bacterial content.

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Abstract

The invention discloses a method for preparing a bacillus amyloliquefaciens Sh1 microcapsule. The method comprises following steps: (1) uniformly mixing the bacillus amyloliquefaciens Sh1 solution, emulsion, suspension or pulp with aseptic gelatin solution; and (2) spray drying the mixed product obtained by step (1), and finally preparing microcapsule powder. The method is characterized in that the air flow in a spray drying process of the step (2) is 600-800 L / h.

Description

technical field [0001] The invention belongs to the technical field of microbial additives for aquatic feeding, and in particular relates to a preparation method of Bacillus amyloliquefaciens Sh1 microcapsules. The Bacillus amyloliquefaciens Sh1 has been disclosed in a Chinese invention patent with the application number 201010264180.6, the applicant is Shanghai Ocean University, and the name is a Saprolegnia antagonist and its application. The corresponding biological deposit information is: CCTCC No: M20101292010.06.02 ). Background technique [0002] In recent years, the rapid development of intensive aquaculture industry has led to the excessive abuse of antibiotics as therapeutic agents and growth promoters for aquatic animal diseases, which has increasingly challenged the safety of aquatic products and caused a series of environmental, health and social problems. At present, developed countries such as Europe, the United States, and Japan have banned antibiotics as fe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J13/04A23K1/16C12N11/04C12R1/01
Inventor 曹海鹏何珊吕利群陈百尧安健伏光辉
Owner SHANGHAI OCEAN UNIV
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