Extraction method and antibacterial activity screening method for fat-soluble active ingredients in insects and arthropods

A technology of active ingredients and extraction methods, applied in the field of fat-soluble extraction technology, to achieve the effect of convenient separation and identification

Inactive Publication Date: 2014-03-26
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a new extraction method and antibacterial activity screening method of fat-soluble active ingredients in insects and arthropods, the technical problem to be solved is to solve the degreasing process of fat-soluble extracts in insects and arthropods problems, laying the foundation for the separation and purification of trace natural effective compounds and the evaluation of biological activity

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  • Extraction method and antibacterial activity screening method for fat-soluble active ingredients in insects and arthropods

Examples

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Effect test

Embodiment 1

[0025] The degreasing process of the black ant fat-soluble extract and the preparation of the test sample of the active ingredient:

[0026] Weigh 0.5-0.8kg of dried black ants and extract them twice with dichloromethane at room temperature for 2-3 days each time (supplemented by ultrasonic operation). The extract was vacuum filtered, the filtrates were combined, and the solvent was recovered under reduced pressure to obtain the insect dichloromethane crude extract.

[0027] Put the obtained crude extract in an evaporating dish, heat in a water bath (40-50°C) for 6-8h, until the dichloromethane in the extract is completely volatilized; mix the crude extract with distilled water at a volume of 1:2-4, Then transfer to a separatory funnel, add an appropriate amount of petroleum ether to shake and extract, let stand and separate layers, take the lower water phase, and extract again according to the above operation, 1-2 times, and recover the water phase; after concentrating the wa...

Embodiment 2

[0030] The degreasing process of the mole cricket fat-soluble extract and the preparation of the test sample of the active ingredient:

[0031] Weigh 0.5-0.8kg of the dried mole cricket body, and extract it twice with dichloromethane at room temperature, each time for 2-3 days (supplemented by ultrasonic operation). The extract was vacuum filtered, the filtrates were combined, and the solvent was recovered under reduced pressure to obtain the insect dichloromethane crude extract.

[0032] Put the obtained crude extract in an evaporating dish, heat in a water bath (50°C) for 6-8 hours, until the dichloromethane in the extract is completely volatilized; mix the crude extract and distilled water at a volume of 1:2-4, and then transfer Put into a separatory funnel, add appropriate amount of petroleum ether to shake and extract, let stand and separate layers, take the lower layer of water phase, extract again according to the above operation, 1-2 times, recover the water phase; after...

Embodiment 3

[0035] The degreasing process of the fat-soluble extract of the grain insect and the preparation of the test sample of the active ingredient:

[0036] Weigh 0.5-0.8 kg of the dried grain worm, and extract it twice with dichloromethane at room temperature for 2-3 days each time (supplemented by ultrasonic operation). The extract was vacuum filtered, the filtrates were combined, and the solvent was recovered under reduced pressure to obtain the insect dichloromethane crude extract.

[0037] Put the obtained crude extract in an evaporating dish, heat in a water bath (40-60°C) for 6-8h, until the dichloromethane in the extract is completely volatilized; mix the crude extract with distilled water at a volume of 1:2-4, Then transfer to a separatory funnel, add an appropriate amount of petroleum ether to shake and extract, let stand and separate layers, remove the lower layer of water phase, extract 1-2 times again according to the above operation, and recover the water phase; after ...

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Abstract

The invention relates to an extraction method and an antibacterial activity screening method for fat-soluble active ingredients in insects and arthropods. The extraction method comprises the following steps: digesting dried insects or arthropods twice with methylene chloride at the room temperature; performing vacuum filtration on a leaching solution, combining filtrates, and recovering a solvent under reduced pressure to obtain insect methylene chloride crude extract; placing the obtained crude extract into an evaporation pan, heating in a water bath at the temperature of 40-50 DEG C for 6-8 hours, mixing the crude extract with distilled water in the volume ratio of 1:(2-4), extracting with petroleum ether, standing for layering, and recovering a water phase; concentrating the water phase, transferring into the evaporation pan, and drying in the water bath at the temperature of 40-50 DEG C to obtain fat-soluble active ingredients from which insect wax is removed in insects and arthropods. In antibacterial activity screening, Gram-positive and Gram-negative bacterial strains are taken as research objects, an antibiotic is taken as a positive control, 70 percent methanol is taken as a blank control, and the antibacterial activity is balanced and compared via the diameter of an inhibition zone measured with a flat band method.

Description

technical field [0001] The present invention relates to a fat-soluble extraction process in insects and arthropods, in particular to a process for extracting fat-soluble active ingredients in insects and arthropods, selecting suitable solvents, suitable temperature and other factors without affecting their Structure and activity of active ingredients. Background technique [0002] The study of insect natural products is a new research direction in the field of natural products in recent years. Compared with the research raw materials, the variety is rich, but the yield is relatively poor; in addition, the ingredients in the insect body are trace amounts, but the structure may be complex; the insect body is in different stages, the secondary metabolites are the material basis, and the possible structural changes, etc., etc. In terms of insect characteristics, the current bottleneck restricting the research of insect natural products is considered to be mainly the establishme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/64A61P31/04C12Q1/18
Inventor 尹卫平高嘉屿任云利李鹏飞李文渊
Owner HENAN UNIV OF SCI & TECH
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