Cervus and cucumis polypeptide pharmaceutical composition and preparation method thereof

A technology of deer melon polypeptide and composition, which is applied in the preparation of deer melon polypeptide pharmaceutical composition for injection, and in the field of deer melon polypeptide pharmaceutical composition for injection, which can solve problems such as inconsistency, achieve inhibited release, improve blood circulation, and reduce capillary The effect of vascular permeability

Inactive Publication Date: 2014-03-26
天津拓飞科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most amino acids are easy to precipitate in alkaline solutions, and the above-mentioned technologies all use alkaline pH values ​​in the extraction process, which are inconsistent with national drug standards.

Method used

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  • Cervus and cucumis polypeptide pharmaceutical composition and preparation method thereof
  • Cervus and cucumis polypeptide pharmaceutical composition and preparation method thereof
  • Cervus and cucumis polypeptide pharmaceutical composition and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Example 1: Preparation of Deer Bone Extract Solution

[0076]Put the deer bones in boiling water for 30 minutes, take them out, remove the fascia and residual meat, and get the deer bones. Take net deer bones and crush them to less than 1cm for later use. Take 30kg of broken bones, add 60kg of water for injection, extract by hot pressing at 121 °C for 2 hours, and filter out the primary extract; then add 60 kg of water for injection, extract by hot pressing at 121 °C for 2 hours, filter out the secondary extract, combine the filtrates, and place overnight. The deer bone extract was taken, centrifuged (4000 rpm, 15 minutes), the supernatant was taken, and the precipitate was discarded. Take the supernatant, carry out fractional ultrafiltration, 100KD, 10KD, 6KD, take the filtrate each time, and discard the concentrate. The filtrate was taken, concentrated under reduced pressure to 10% of the total volume, collected the concentrated solution, adjusted the pH value to 6....

Embodiment 2

[0077] Example 2: Preparation of Melon Seed Extract Solution

[0078] Take the melon seeds, put them in water for injection, rinse 3-4 times repeatedly, empty the water, pick out the sundries, and get the melon seeds for use. Get net melon seeds 15kg, add 30kg water for injection, grind into melon seeds homogenate with a colloid mill. Take the melon seed homogenate, extract by hot pressing at 121°C for 2 hours, and filter out the primary extract; then add 30 kg of water for injection, continue extracting by hot pressing at 121°C for 2 hours, filter out the secondary extract, combine the filtrates, and place overnight. Take the melon seed extract, centrifuge (4000 rpm, 15 minutes), take the supernatant, and discard the precipitate. Take the supernatant and carry out grading ultrafiltration: 100KD, 10KD, 6KD, take the filtrate each time, and discard the concentrate. Take the final filtrate, concentrate under reduced pressure to 10% of the total volume, collect the concentrated...

Embodiment 3

[0079] Example 3: Pharmaceutical composition 1

[0080] Formulation prescription:

[0081] The deer bone extract solution of Example 1 26.7g (calculated by deer bone polypeptide)

[0082] The melon seed extract solution of Example 2 13.3g (calculated by melon seed polypeptide)

[0083] 20% excipient solution 5000ml

[0084] 5%NaOH (or 5%HCl) appropriate amount

[0085] Add water for injection to 20000ml

[0086] The deer bone extract solution prepared in Example 1 and the melon seed extract solution prepared in Example 2 were mixed according to the weight ratio of deer bone polypeptide and melon seed polypeptide in a ratio of 2:1, and 5000ml of excipient (dextran 40, glucose or lactose) solution was added. , adjust the pH value of the solution to 5.5-7.0 with an appropriate amount of 5% NaOH or 5% HCl, add water for injection to the full amount, and prepare a 4mg preparation each through the steps of canning, lyophilization, and capping.

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Abstract

The invention discloses a cervus and cucumis polypeptide pharmaceutical composition for injection. The pharmaceutical composition contains a deer bone extract, a muskmelon seed extract and one or more of excipients, wherein the weight ratio of deer bone polypeptide to muskmelon seed polypeptide is (2:1)-(6:1). The invention further discloses an application of the cervus and cucumis polypeptide pharmaceutical composition for injection in preparation of drugs for treating rheumatism, rheumatoid arthritis, ankylosing spondylitis, various types of fractures, wound repair and low back and leg pain. As shown in experimental results, the cervus and cucumis polypeptide pharmaceutical composition for injection has excellent functions of alleviating pain, eliminating swelling and promoting fracture healing.

Description

technical field [0001] The present invention relates to a deer melon polypeptide pharmaceutical composition for injection and a preparation method thereof. More specifically, the present invention relates to a deer melon polypeptide pharmaceutical composition for injection containing deer bone polypeptide and melon seed polypeptide. The invention also relates to a preparation method of the deer melon polypeptide pharmaceutical composition for injection. Background technique [0002] Deer melon polypeptide is formulated with extracts of deer bone and melon seeds. The motherland medicine believes that deer bone is sweet and slightly hot, and has the functions of nourishing deficiency, strengthening muscles and bones, and curing rheumatism and limb pain and cold paralysis of muscles and bones. Sanjie, pain relief and other functions, the two are formulated for dispelling cold, expelling rheumatism, and analgesia, so they have a therapeutic effect on rheumatoid arthritis or rhe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/42A61P19/02A61P29/00A61P19/08A61K35/32
Inventor 李虹
Owner 天津拓飞科技有限公司
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