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Method for preparing compound microbial inoculant by microbially decomposing Bayan Obo potassium-rich slate

A technology of microbial decomposition and compound inoculants, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of increased labor and production costs, no added mineral elements, etc., to reduce production labor, Reduced production cost and strong adaptability

Active Publication Date: 2014-03-26
INNER MONGOLIA UNIV OF SCI & TECH
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AI Technical Summary

Problems solved by technology

[0008] However, most of the commercially available EM bacteria agents only provide microbial resources without adding mineral elements.
Therefore, when applying EM bacteria, a certain amount of other fertilizers must be applied to supplement the mineral elements required for plant growth, which undoubtedly increases the amount of labor and production costs in production.

Method used

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Embodiment Construction

[0037] The first step, strain activation: In order to obtain strains with good growth and high efficiency in decomposing ore, the Bacillus megaterium purchased from GIMCC ( Bacillus megaterium , AS1.459; GIMCC GIM1.14) were inoculated on solid medium (5 g peptone, 3 g beef extract, 5 g NaCl, 20 g agar, 1 L deionized water, pH 7.0~ 7.2) above, activate for 3~5 generations in a constant temperature incubator at 30°C.

[0038] The second step, preparation of bacterial solution: pick a single colony that grows well on the solid medium in the first step and inoculate it into the liquid medium (waste molasses 20 g, (NH 4 ) 2 SO 40.5 g, MgSO 4 ·7H 2 O 0.3 g, NaCl 0.3 g, KCl 0.3 g, FeSO 4 7H2O 0.3 g, MnSO 4 ·7H 2 O 0.3 g, Ca 3 (PO 4 ) 2 5 g, 1 L of deionized water; pH value 7.0~7.3), 30 °C, 200 rpm / min shaker culture for 5-6 days, to obtain Bacillus megaterium bacteria liquid, stored in 4 °C refrigerator for later use.

[0039] The third step, microbial leaching: in o...

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Abstract

The invention discloses a method for preparing a compound microbial inoculant by microbially decomposing Bayan Obo potassium-rich slate, which comprises the following steps: decomposing Bayan Obo potassium-rich slate ores in a liquid fermentation culture medium by using Bacillus megaterium (AS1.459, GIMCCGIM1.14), thus obtaining a leach solution containing potassium, phosphorus, sulfur, minor elements, rare-earth elements and other minerals required by plants; and mixing the leach solution and a commercially available EM microbial inoculant according to a volume ratio of 1:1, thus obtaining the compound microbial inoculant containing multiple mineral elements (potassium, phosphorus and sulfur). The method causes less pollution and is low in energy consumption; and the Bayan Obo potassium-rich slate ores are used as raw materials, thus lowering the cost and eliminating the pollution of rejected ores to environment. The raw materials (Bayan Obo potassium-rich slate ores) in the method can be sufficiently decomposed, thereby greatly improving the utilization ratio of the ores.

Description

[0001] technical field [0002] The invention relates to a method for decomposing Baiyun Obo potassium-rich slate by microorganisms to prepare composite bacterial agents, in particular to a method for using microorganisms to decompose Baiyun Obo potassium-rich slates to prepare composite bacterial agents containing various mineral elements. Background technique [0003] Most of the potassium-rich slate in Baiyun Obo is within the mining boundary of the Baiyun Obo Iron Mine. With the mining of iron ore, some potassium-rich slate ores have been mined at the same time. [0004] For decades, a large amount of potassium-rich slate ore has been piled up as waste rock in the quarry because there is no better way to use it, which not only causes waste of resources, but also causes great pollution to the environment, which is not conducive to sustainable development and energy saving Environmental friendly. [0005] At present, the methods used to process ore are mainly traditional ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12R1/11
Inventor 郑春丽陈敏洁肖作南王建英袁浩容王亚琴
Owner INNER MONGOLIA UNIV OF SCI & TECH
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