Analysis identification method of ophiocordyceps sinensis through infrared spectrum

An infrared spectrum analysis, Cordyceps sinensis technology, which is applied in the direction of material analysis, analysis materials, measuring devices and other directions by optical means, can solve the problem of indistinguishable authenticity of the whole Cordyceps sinensis grass, and achieves rapid testing, rapid analysis methods, and accurate results. Effect

Active Publication Date: 2014-03-26
QINGHAI SPRING MEDICINAL RESOURCES TECHNOLOGY CO LTD
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Problems solved by technology

[0004] What the present invention aims to solve is the technical problem that the whole

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  • Analysis identification method of ophiocordyceps sinensis through infrared spectrum
  • Analysis identification method of ophiocordyceps sinensis through infrared spectrum
  • Analysis identification method of ophiocordyceps sinensis through infrared spectrum

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Effect test

Embodiment 1

[0058] Example 1 Drawing of the standard spectrum of the whole plant of Cordyceps sinensis, collection of the necessary characteristic peaks of the infrared spectrum of the whole plant of Cordyceps sinensis and the collection of common characteristic peaks of the infrared spectrum of the whole plant of Cordyceps sinensis, and determination of the lower threshold of the correlation coefficient

[0059] 1) Sample source and processing

[0060] Select 15 samples of Cordyceps sinensis from Yushu Prefecture in Qinghai, 15 from Guoluo Prefecture, 15 from Hainan Prefecture, 15 from Tibet, 15 from Sichuan and 8 from Gansu, and select samples of uniform size for each origin.

[0061] The sample was processed according to the relevant regulations of the Pharmacopoeia of the People's Republic of China. The insect body part of the sample was naturally dried and then pulverized. The fine powder after passing through a 200-mesh sieve was used for infrared spectrum testing.

[0062] 2) Infra...

Embodiment 2

[0084] Embodiment 2 Distinguishing authenticity experiment

[0085] 1) Sample source and processing

[0086] The numbers of commercially available Cordyceps Gurney are G1, G2, G3, and G4.

[0087] The sample was processed in accordance with the relevant regulations of the Pharmacopoeia of the People's Republic of China. The whole herb part of the sample was naturally dried and then pulverized. The fine powder passed through a 200-mesh sieve was used for infrared spectrum testing.

[0088] 2) Infrared Spectroscopy Instrument

[0089] All samples were tested using a Spectrum One Fourier transform infrared spectrometer from PerkinElmer, USA.

[0090] Sample test parameters: spectral scanning range 4000 ~ 400cm -1 , spectral resolution 4cm -1 , each spectrum is accumulated and scanned 16 times, and the interference of carbon dioxide and water vapor is deducted in real time.

[0091] 3) Infrared Spectroscopy Test Method

[0092] The samples were tested by potassium bromide (K...

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Abstract

The invention relates to the field of analytical chemistry, particularly to an analysis identification method of ophiocordyceps sinensis through an infrared spectrum, and aims to solve the technical problem that the authenticity of the existing ophiocordyceps sinensis is difficult to distinguish. In order to solve the above technical problem, the invention provides a technical scheme to rapidly and accurately distinguish ophiocordyceps sinensis. The analysis identification method through the infrared spectrum comprises the following steps: a, drawing a standard spectrum of ophiocordyceps sinensis; b, collecting an essential characteristic peak and a common characteristic peak of an infrared spectrum of ophiocordyceps sinensis; c, distinguishing the essential characteristic peak and similarity of the infrared spectrum of measured ophiocordyceps sinensis. The invention provides a novel method for rapidly and accurately distinguishing ophiocordyceps sinensis.

Description

technical field [0001] The present invention relates to the field of analytical chemistry. Specifically, it relates to an infrared spectrum analysis and identification method for the whole plant of Cordyceps sinensis. Background technique [0002] Cordyceps sinensis is a complex of the subunit and larvae of Cordyceps sinensis (Berk.) Sacc., a fungus of the ergot family Fungus Cordyceps sinensis (Berk.) Sacc. Ginseng and deer antler are known as the three major tonics in China. [0003] The growth environment of Cordyceps sinensis is special, and it is mainly distributed in alpine meadows and alpine shrubs at an altitude of 3000-5000 meters on the Qinghai-Tibet Plateau in my country. Qinghai, Tibet, Sichuan, Gansu and Yunnan are the main production areas of Cordyceps sinensis. The output and quality of Qinghai ranks first among all provinces and autonomous regions. Yushu and Guoluo are the main production areas of Cordyceps sinensis in Qinghai. Cordyceps sinensis cannot be...

Claims

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Application Information

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IPC IPC(8): G01N21/3563
Inventor 张雪峰徐丽王晓平
Owner QINGHAI SPRING MEDICINAL RESOURCES TECHNOLOGY CO LTD
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