Method for identifying zygophyllum xanthoxylum
A kind of overlord and species technology, applied in the field of plant species identification, can solve the problems of the limitation of overlord identification, the distinction of unfavorable varieties, and the impact on the accuracy of identification, and achieve the effect of rapid identification, easy operation and strong practicability
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Embodiment 1
[0027] This embodiment has determined the ribosomal DNA ITS sequence from 9 place of origin Bawang (Paragon, Bayin Wendur, Zongbieli, Chaogetu Huresumu, Bayannuorigong, Xini Town, Baiyin Engel, Zhuozhuoshan, Mu Us), the steps are as follows:
[0028] 1. Sample collection and processing
[0029]The mature Bawang leaves (collected locally) of the current year were used as the material for extracting genomic DNA, and they were dried in a drying box filled with silica gel.
[0030] 2. Extraction of total genomic DNA
[0031] The kit (EE111-12) provided by Beijing Quanshijin Biotechnology Co., Ltd. was used
[0032] ①Place EB in a water bath (60-70°C) for constant temperature preheating;
[0033] ② Cut a small amount of sample (approximately 30 mg of dried tissue) into a 2ml centrifuge tube, put several small steel balls into the centrifuge tube at the same time, place the centrifuge tube in a plant high-speed tissue grinder for full grinding, centrifuge program Set 800rpm, 50s...
Embodiment 2
[0061] The difference from Example 1 is only that the contrast plants are replaced with pearl pigtail, red sand, quadrangle wood, caragana, and holly. The ITS sequences of the above species obtained through the NCBI database were used as reference sequences for comparison and cluster analysis with the obtained Bawang ITS sequences. The sequence comparison results are as follows Figure 4 As shown, the homology rate of the sequence comparison is greater than 95%, which can be regarded as originating from the same species, so that different germplasm resources can be distinguished according to the comparison results, and the purpose of identifying the overlord can be achieved. Clustering results such as Figure 5 As shown, the homology rate of Bawang in 9 regions is high, and they are clustered into one branch, and other species are clustered into another branch, but the homology with Bawang is far away, so they can be distinguished from Bawang. In summary, the method of the pr...
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