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EV71 (Enterovirus 71) VP1 and RdRp (RNA dependent RNA polymerase) protein specific T cell epitope peptide as well as application thereof

A specific, epitope peptide technology, applied in the medical field, to achieve the effect of considerable development and application prospects

Inactive Publication Date: 2014-04-02
党双锁
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows for the induction of antibodies that specifically target certain proteins found on virus particles called viral coat protein (VC) or envelope glycoprotein (EVI). These specialized molecules are able to stimulate helper lymphocytes to help them fight off other infectious agents like bacteria. They also allow these same types of white blood cells to make their way through this process into new tissues where they attack foreign invaders more effectively than before. Overall, this technology provides an effective method for creating protective measures against various diseases caused by harmful microorganisms such as measles disease coronavirus-19.

Problems solved by technology

This patented problem addressed in the patents relates to studying how epidemics like EVD-1 affect humans' health and wellness. Specifically, identifying key factors involved in causing these diseases involves analyzing their molecular organization patterns or interactions between them.

Method used

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  • EV71 (Enterovirus 71) VP1 and RdRp (RNA dependent RNA polymerase) protein specific T cell epitope peptide as well as application thereof
  • EV71 (Enterovirus 71) VP1 and RdRp (RNA dependent RNA polymerase) protein specific T cell epitope peptide as well as application thereof
  • EV71 (Enterovirus 71) VP1 and RdRp (RNA dependent RNA polymerase) protein specific T cell epitope peptide as well as application thereof

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Embodiment Construction

[0056] Embodiments of the present invention are described below, and embodiments do not constitute a limitation of the present invention: the applicant designs according to the EV71-Hubei-09-China virus strain (gene number: GU434678.1) EV71C4 subtype VP1 and RdRp protein amino acid gene sequence, Synthesize 36 overlapping peptides covering VP1 protein and 57 overlapping peptides of RdRp protein, and use overlapping peptide synthesis technology (PEPscreen; Sigma-Aldrich) to synthesize overlapping peptides of VP1 and RdRp protein regions, Minute They contain 297 and 462 amino acids, each peptide consists of 18 amino acids, and 10 amino acids overlap between adjacent peptides. The overlapping peptides have a purity >95%, dissolved in RPMI 1640 at a concentration of 20 mg / ml, and stored in liquid nitrogen at -80°C for future use.

[0057] In vitro IFN-γElispot method was used to detect overlapping peptides covering EV71 VP1 and RdRp protein sequences as inducing antigens to induc...

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Abstract

The invention discloses EV71 (Enterovirus 71) VP1 and RdRp (RNA dependent RNA polymerase) protein specific T cell epitope peptide as well as application thereof. The EV71 VP1 and RdRp protein specific T cell epitope peptide can induce T cells of an EV71 infected person to generate cellular immune response and secrete cytokine IFN-gamma, and the specific amino acid sequence is presented from SEQ ID NO.1 to SEQ ID NO.24 as follows. The EV71 VP1 and RdRp protein specific T cell epitope peptide can be applied to preparation of T cell epitope vaccines or to induction and generation of immune response of the specific T cell of epitope peptide, and has good application prospect in the field of EV71 specific immunity prevention and treatment.

Description

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Claims

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Application Information

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Owner 党双锁
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