Compound microbial activator and preparation method and application thereof

A technology of composite microorganisms and microbial agents, applied in the field of environmental microorganisms, can solve the problems that have not yet been reported publicly, and achieve the effect of promoting high-temperature fermentation process, fast temperature rise, and improving population

Active Publication Date: 2014-04-02
大地绿源环保科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no public reports of this type of compost inoculant

Method used

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  • Compound microbial activator and preparation method and application thereof
  • Compound microbial activator and preparation method and application thereof
  • Compound microbial activator and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Activation of embodiment 1 microbial strain

[0039] 1) Transfer the slant of the UTM401 production strain stored at 4°C to the LB medium plate, and cultivate it at 30°C until it becomes abundant.

[0040] 2) Transfer the slants of UTM501 and UTM601 production strains stored at 4°C to LB medium plates, and culture them at 55°C until they become rich.

[0041] 3) Transfer the UTM01, UTM02, and UTM03 production strains stored at 4°C to the LB medium plate, and culture them at 60°C, 60°C, and 65°C until they are abundant.

[0042] 4) Transfer the UTM01, UTM02, and UTM03 production strains stored at 4°C to the LB medium plate, and cultivate them at 65°C until they become rich.

[0043] 5) Transfer the UTM01, UTM02, and UTM03 production strains stored at 4°C to the LB medium plate, and culture them at 60°C, 65°C, and 65°C until they are abundant.

[0044] 6) Transfer the slant of the UTM801 production strain stored at 4°C to the improved YTPG medium plate, and cultivate it...

Embodiment 2

[0045] The preparation of embodiment 2 microbial fermentation seed liquid

[0046] 1) Preparation of UTM401 fermented seed solution

[0047] Use a sterile glass spatula to transfer all the UTM401 colonies that have been activated and cultured on the LB plate to a 5000ml Erlenmeyer flask containing 2000ml LB liquid medium, and culture them on a shaker at 30°C with a speed of 160r / min for 3 days , at this time the OD600 was 1.8, stop the cultivation, this is the primary fermentation seed liquid.

[0048] Inoculate the primary fermentation seed liquid into the fermentation seed tank to carry out fermentation culture. Fermentation conditions are: inoculation amount (v / v) 10%, canned amount (v / v) 80%, intermittent ventilation and stirring: every 4 hours aeration for 10 minutes, ventilation rate (v / v) 1:1, every Stir for 10 minutes every 8 hours, rotate at 160 rpm, and cultivate at 30°C for 3 days. The OD600 was detected to be 2.5, and the cultivation was stopped. This is the UTM4...

Embodiment 3

[0058] The preparation of embodiment 3 fermentation activators

[0059] Take cassava skin, cassava distiller's grains, pig manure, plant ash, and cornmeal and mix them in a weight ratio of 2:2:2:0.8:0.2, so that the height of the fermentation pile is 1.4 meters, and the fermentation temperature is checked at multiple points every day. According to the temperature change, inoculate each fermentation liquid equivalent to 0.3% of the dry weight of the initial material. Before inoculation, use an appropriate amount of cornmeal to absorb each fermentation seed liquid, and stir to mix evenly after inoculation. The inoculation time of each bacteria is: inoculate UTM501 and UTM601 when the highest fermentation temperature reaches 55°C, inoculate UTM01 when the highest fermentation temperature reaches 65°C, inoculate UTM801 when the highest fermentation temperature reaches 70°C, and maintain at 70°C for 2 days; Inoculate UTM401 when the maximum fermentation temperature drops to 38°C, t...

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Abstract

The invention relates to a compound microbial activator and a preparation method and application thereof. The compound microbial activator is prepared from the microbial agents of groups A and B, wherein the group A comprises one or more of bacillus methylotrophicus UTM401, anaerobic bacillus UTM501, anaerobic bacillus UTM601 and extreme thermophilic bacteria UTM801; the group B comprises one or more of geobacillus UTM01, geobacillus UTM02 and geobacillus UTM03. The compound microbial activator provided by the invention can greatly improve the microbial colony at the early stage of composting, enhances the metabolic activity, realizes fast temperature increase, promotes the high-temperature fermentation progress and remarkably promotes the composting process and improves the composting quality.

Description

technical field [0001] The invention relates to the technical field of environmental microorganisms, in particular to a composite microorganism activator and its preparation method and application. Background technique [0002] Compost inoculation refers to a method of isolating and domesticating beneficial microorganisms from diverse environments or materials, and inoculating them into the compost through cultivation and fermentation to form bacterial agents. These single or compound microbial agents used for compost inoculation are usually called compost inoculants or fermentation inoculants. At present, compost inoculants have been widely used in organic solid waste composting fermentation, and there are new inoculants under development, but most of them focus on the development of mesophilic aerobic microorganisms, and there are still some limitations in application. [0003] On the one hand, when the composting temperature reaches above 60°C, the composting microorgani...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F17/00C12R1/01C12R1/07
CPCY02W30/40
Inventor 刘永跃周顺桂许宜北汪涌
Owner 大地绿源环保科技(北京)有限公司
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