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Application of beta-aminobutyric acid in inducing tea tree to generate resistance on tea geometrid

A technology of aminobutyric acid and tea inchworm, which is applied in the agricultural field to achieve the effects of reducing the emergence rate, reducing the body weight and increasing the total development period of larvae

Active Publication Date: 2015-02-18
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

β-aminobutyric acid and benzothiadiazole are two typical chemical inducers, which are mainly used to induce plant disease resistance, but there are no reports on inducing tea plants to enhance resistance to tea geometrids.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The tea trees were sprayed with 100mg / L, 200mg / L, and 300mg / L β-aminobutyric acid solutions respectively, and the water treatment was used as a control, and the amount of solution per mu was 100 liters. Spray twice within 48 hours. Sampling and analysis after the last spraying 48h showed that the activity of phenylalanine ammonia lyase (PAL) in the drug treatment group increased by 17.1%, 36.9% and 63.3%, respectively, and the activity of polyphenol oxidase (PPO) compared with the control (clean water treatment). Respectively increased by 42.9%, 48.4% and 69.4%, the enhanced PAL activity can lead to the increase of tea polyphenol synthesis, affecting the feeding choice of insects, and the enhanced PPO activity can promote the oxidation of polyphenols, affecting the digestion of insects.

[0019] One week after the last spraying, the shoot lengths of the test drug treatment group and the matched group (clear water treatment) were sampled, and compared with the matched gr...

Embodiment 2

[0021] The tea trees were sprayed with 200 mg / L, 300 mg / L, and 400 mg / L β-aminobutyric acid solutions respectively, and the water treatment was used as a control, and the amount of liquid used was 100 liters per mu. Spray twice within 48 hours. 48 hours after the last spraying, the leaves of drug treatment and non-medication treatment were collected at the same time, and the 2nd instar tea geometrid larvae were continuously fed, and the survival rate, pupation rate, eclosion rate and total developmental period of each group of larvae were counted. The results showed that using After β-aminobutyric acid treatment, the survival rate of larvae decreased by 11.6% to 18.6%. From the 3rd to 5th instar, the average food intake of larvae tended to increase in all treatments (medicine group and control group), except for 200mg / L The food intake of the larvae from the treatment group to the 5th instar was about 7% higher than that of the control group, 200mg / L was used to treat the 3rd ...

Embodiment 3

[0023] Mix 150 mg / L 2,1,3-benzothiadiazole solution and 400 mg / L β-aminobutyric acid solution in a volume ratio of 1:2 and spray the tea tree, and use water treatment as a control. Spray twice within 48 hours. 48 hours after the last spraying, the activity of phenylalanine ammonia lyase (PAL) was increased by 76.3%, and the activity of polyphenol oxidase (PPO) was increased by 49.4%.

[0024] 48 hours after the last spraying, the leaves treated with and without treatment (control group) were collected at the same time, and the 2nd instar tea geometrid larvae were continuously fed to the 5th instar larvae, and the two most important protein-digesting enzymes in the insects were sampled and determined—— Chymotrypsin-like activity and strong alkaline tryptase activity, the results compared with the control group, chymotrypsin-like activity decreased by 41.7%; strong alkaline tryptase activity decreased by 17.2%. The larval survival rate decreased by 18.7%, the pupation rate decr...

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PUM

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Abstract

The invention discloses an application of beta-aminobutyric acid in inducing a tea tree to generate resistance on a tea geometrid. The application comprises a method for treating the tea tree by using the beta-aminobutyric acid, a mixture of the beta-aminobutyric acid and benzothiadiazole and a mixture of the beta-aminobutyric acid and the derivative of the benzothiadiazole. By adopting the method to treat the tea tree, the activity of PPO (Polyphenol Oxidase), POD (Peroxidase) and PAL (Phenylalanine Ammonia Lyase) which are contained in a fresh tea leaf tissue is outstandingly enhanced, and the total amount of tea polyphenol, the content of catechin and the content of amino acid are increased; after fresh tea leaves treated through the method are fed, the tea geometrid is increased in developmental duration, the feeding amount and weight of larvae are outstandingly reduced, and the survival rate, the pupation rate and the eclosion rate are reduced at different degrees, so that the purpose of preventing and controlling the tea geometrid is contribute to realize.

Description

technical field [0001] The invention belongs to the technical field of agriculture, and specifically relates to the application of beta-aminobutyric acid in inducing resistance of tea plants to tea geometrids. Background technique [0002] Tea geometrids, commonly known as arch worms and tea geometrid moths, have many generations per year, reproduce quickly, and spread rapidly. They eat leaves with larvae. All tea-producing provinces south of the Yangtze River Basin have occurred, especially in Jiangsu, Zhejiang, Anhui, Hunan and other provinces. The control of diseases and insect pests is undoubtedly an important part of tea production. At present, chemical control is mainly used in various tea areas. [0003] The existing methods of preventing and controlling tea inchworm mainly include: [0004] (1) Scrape off the egg mass between the branches or in the nearby woods; (2) Trapeze and kill with lights and sex attractants. Since the adults of tea geometrid have phototaxis,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N37/44A01N43/82A01P7/04A01G13/00
Inventor 李荣林李珍珍
Owner JIANGSU ACAD OF AGRI SCI
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