Research method for the effect of potassium fertilizer on the proteomics of potato infested by insects

A potato protein, potato technology, applied in the directions of botanical equipment and methods, horticultural methods, scientific instruments, etc., to achieve the effect of enhancing the mechanism of insect resistance and deepening understanding

Inactive Publication Date: 2017-01-04
集宁师范学院
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Problems solved by technology

Although there have been some studies on the relationship between jasmonic acid signaling and potato insect resistance, systematically combining potassium nutrition with potato jasmonic acid signal transmission after insect damage, by studying the potato's perception of external potassium concentration changes and the impact on jasmonic acid synthesis, There is no report on the regulation of its protein expression

Method used

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  • Research method for the effect of potassium fertilizer on the proteomics of potato infested by insects
  • Research method for the effect of potassium fertilizer on the proteomics of potato infested by insects
  • Research method for the effect of potassium fertilizer on the proteomics of potato infested by insects

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Embodiment

[0023] In this example, the potato variety used is Favorita, which is native to the Netherlands and is an early-maturing, high-yielding variety. The seeds were purchased from Ulanqab Seed Company, Inner Mongolia. In May 2011, the seeds of Kexin No. 1 were sown in plastic pots. Peach aphid was provided by the Population Ecology and Global Climate Change Group, Institute of Zoology, Chinese Academy of Sciences.

[0024] The process is as follows:

[0025] (1) Select 320 pots of potato Fevorita with two leaves in the same growth pattern, and divide them into two groups, i.e. the control group (no potassium application group) and the treatment group (potassium application group). Potassium fertilizer is potassium sulfate, and its dosage is calculated based on the minimum potassium application rate of 75,000 plants / ha of potatoes. Each group was divided into 4 levels of potassium application, and the applied potassium sulfate was 0 (CK), 4, 6 and 8 g / plant respectively.

[0026] ...

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Abstract

The invention relates to a research method for the influence of potassium fertilizer on the proteomics of potato pests, and belongs to the field of biotechnology. The specific steps are to select potatoes with the same growth and two leaves respectively, and divide them into two groups, namely the control group (no potassium application group) and the treatment group (potassium application group), and the potassium fertilizer applied to the potassium application group is potassium sulfate. The treated potato plants included four treatments: control, potassium application, inoculation, and potassium application+inoculation. Observe and record the population of green peach aphid, and measure the content of jasmonic acid, protease inhibitor activity and enzyme activity related to insect resistance in different potato leaves. Potassium application of 6g / plant has the strongest inhibitory effect on green peach aphid. The iTRAQ technology was used to study the expression of proteomics in potato leaves after different treatments, and to clarify the mechanism of potassium application on potato induced defense. The invention gives full play to the natural resistance and induced resistance of the potato, reveals the mechanism of potassium fertilizer enhancing the insect resistance of the potato, and has great significance for carrying out the ecological regulation of aphids.

Description

technical field [0001] The invention relates to a research method for the influence of potassium fertilizer on the proteomics of potato pests, and belongs to the field of biotechnology. Background technique [0002] Potato (Solanum tuberosum) is the fourth largest crop in the world, and it is also an important high-yield grain and vegetable crop in the Inner Mongolia Autonomous Region, which is of great significance in all aspects of the national economy. As the "Potato Capital of China", Ulanqab is an important potato planting, production and processing base in the country. Potato products as edible fresh potatoes, seed potatoes and processed raw potatoes are exported to domestic and Southeast Asian countries, and become a pillar industry for farmers in the city to get rid of poverty and build new countryside. Potato yield and quality are comprehensively affected by various factors such as variety, fertilization, climate, and pests and diseases. Among them, green peach aph...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G7/06G01N27/62G01N30/02G01N33/68
Inventor 任琴白雪马晓林徐松鹤杨晓红
Owner 集宁师范学院
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