A method for determining fingerprints of traditional Chinese medicine preparations for treating coronary heart disease
A technology of traditional Chinese medicine preparations and fingerprints, applied in the field of medicine, can solve the problems of insufficient internal quality of traditional Chinese medicine preparations, and achieve the effects of improving neurological function defects, good reproducibility and reliable methods
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Embodiment 1
[0033] Embodiment 1: the establishment of Chinese medicine preparation fingerprint spectrum assay method of the present invention
[0034] 1.1 Instruments and reagents
[0035] Agilent1260 (quaternary pump G1311B; autosampler G1329B; column thermostat G1316A; diode array detector G4212B; LAB open chromatography data workstation); electronic analytical balance (model BP211D, d=0.01mg, German Sartorius Balance Co., Ltd. ); Ultrasonic Cleaner (Model: KQ-250DE, Kunshan Ultrasonic Instrument Co., Ltd.); Agilent ZORBAX Extend-C 18 Chromatography column (250mm×4.6mm, 5μm).
[0036] 1.2 Reagent
[0037] 10 batches of Chinese medicine preparations of the present invention are produced by Shaanxi Buchang Pharmaceutical Co., Ltd., and the sample numbers of 10 batches are respectively Y 1 , Y 2 , Y 3 , Y 4 , Y 5 , Y 6 , Y 7 , Y 8 , Y 9 , Y 10 , National Pharmaceutical Standard Z20020055, which is a capsule. Methanol was chromatographically pure (Fisher Reagent Company, USA), ...
Embodiment 2
[0070] Embodiment 2 The establishment of the fingerprint of the Chinese medicine preparation of the present invention
[0071] 2.1 Preparation of the test solution
[0072] Take about 1 g of the contents of 10 different batches of traditional Chinese medicine preparations, accurately weigh them, put them in a 100 mL conical flask with a stopper, add 10 mL of 70% methanol, weigh, ultrasonically (100W, 45kHz) for 30 minutes, let cool, and make up with 70% methanol Lose the lost weight, shake well, filter through a 0.45μm microporous membrane, and take the subsequent filtrate.
[0073] 2.2 Chromatographic conditions:
[0074] Chromatographic column: Agilent ZORBAX Extend-C18 column (250mm×4.6mm, 5μm); column temperature: 35°C, flow rate: 1.0mL min -1 , with methanol as the mobile phase A and 0.4% phosphoric acid aqueous solution as the mobile phase B, perform gradient elution according to the volume concentration preparation ratio in Table 5.
[0075] Table 5 Mobile phase gradie...
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