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Preparation method of rabbit anti-tree shew antiserum

A Chinese Burmese tree shrew antiserum technology, applied in the field of antiserum of Burmese tree shrews, can solve the problems of large error, high preparation cost, and poor effect, and achieve the effect of small error, good effect, and large economic benefits

Inactive Publication Date: 2014-05-21
YUNNAN NORMAL UNIV
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Problems solved by technology

[0005] The purpose of the embodiments of the present invention is to provide a method for preparing rabbit antiserum against Burmese tree shrew, which aims to solve the problems of high preparation cost, large error and poor effect of UCP1 content determination in traditional Burmese tree shrew antiserum

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  • Preparation method of rabbit anti-tree shew antiserum
  • Preparation method of rabbit anti-tree shew antiserum
  • Preparation method of rabbit anti-tree shew antiserum

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Embodiment Construction

[0032] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0033] The application principle of the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0034] Such as figure 1As shown, a method for preparing rabbit antiserum against Burmese tree shrew, the method steps include: selection of experimental animals S101, serum preparation method S102, primary immunization S103, booster immunization S104, serum titer test S105; The above-mentioned serum preparation method S102 step process includes: blood collection S201, hemostasis S202, serum preparation S203, and the effect of control serum S204; the serum titer ...

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Abstract

The invention discloses a preparation method of rabbit anti-tree shew antiserum. The method comprises the steps of selecting an experimental animal, preparing serum, carrying out primary immunization and booster immunization and testing serum titer; the preparation method of the serum comprises the steps of collecting blood, stopping bleeding, preparing the serum and comparing the function of the serum; a bidirectional immune diffusion method is adopted by the test for the serum titer. According to the preparation method of the rabbit anti-tree shew antiserum, the content of tree shew uncoupling protein-1(UCP1) can be measured by the prepared antiserum, and the result error is generally within the range of 3-5%, so that the error range is reduced; after the steps of primary immunization and booster immunization are utilized, the production cost is lowered, the UCP1 content measurement effect is good, and the economic benefit is high.

Description

technical field [0001] The invention belongs to the technical field of Burmese tree shrew antiserum, and in particular relates to a preparation method of rabbit anti- Burmese tree shrew antiserum. Background technique [0002] Obesity is a serious threat to human health in the world today, which can lead to a significant increase in the risk of some major diseases that endanger human health. Obesity is mainly the result of a large accumulation of white adipose tissue (WAT) due to the incoordination between energy intake and expenditure. In the human body, obesity is inevitable due to overeating and little exercise. According to the World Health Organization, more than 1.5 billion people are currently overweight (body mass index, BMI>25), and more than 500 million people are obese (BMI>30), and these numbers are still increasing (World Health Organization2013) . Recently, the FDA banned rimonabant and sibutramine, which greatly restricted drug treatment. Therefore, ...

Claims

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Application Information

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IPC IPC(8): A61K49/00G01N33/68
Inventor 王政昆朱万龙张浩高文荣章迪沐远
Owner YUNNAN NORMAL UNIV
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