Tobacco squalene epoxidase protein, tobacco squalene epoxidase gene and its application

Abstract

The invention discloses a tobacco squalene epoxidase protein with an amino acid sequence shown in SEQIDNO:1. A tobacco squalene epoxidase gene NtSE is a key control gene in the synthetic route of sterol in tobaccos. The gene is specifically highly expressed in young tissues, a built virus mediated gene silenced carrier TRV-NtSE is inoculated to a Ben's tobacco, and then a plant with a high silencing efficiency is obtained; through detecting the content of phytosterol in the Ben's tobacco, a situation that the sterol content of the gene silenced plant is significantly reduced by 21.37% is found, which shows that the silencing of the gene can significantly reduce the sterol content of the plant. The tobacco squalene epoxidase gene NtSE is a key control gene in the synthetic route of sterol in tobaccos, can be used for regulating the sterol content of tobaccos, and facilitates the genetic engineering breeding of low-sterol high-quality tobaccos.

Description

technical field [0001] The invention relates to tobacco sterol synthesis and regulation key genes, in particular to tobacco squalene epoxidase gene TTSE Application in reducing tobacco sterol content. Background technique [0002] Tobacco is a plant of the genus Nicotiana in the family Solanaceae of the dicotyledonous plant class Picoflowers. There are more than 60 species of Nicotiana. Among the two cultivated species, common tobacco (also known as Nicotiana tabacum) occupies the main area, and Nicotiana rustica occupies a small area. Cultivated tobacco can be divided into six types: flue-cured tobacco, sun-cured tobacco, air-dried tobacco, burley tobacco, oriental tobacco and yellow flower tobacco according to the quality characteristics of tobacco leaves, biological characteristics and cultivation methods, among which flue-cured tobacco is the most widely cultivated common tobacco. my country's flue-cured tobacco planting area and total output both rank first in the wor...

AI Technical Summary

Problems solved by technology

Since the structure of sterols all contain hydroxyl groups, the tetracyclopentene[α]phenanthrene ring structure of its parent can form fused-ring aromatic hydrocarbons during pyrolysis. Therefore, sterols in tobacco are potential substances that affect human health, so reduce The sterol content in mature tobacco leaves is also one of the effective ways to reduce the tar and harm of cigarettes. At present, there is no effective method to effectively reduce the sterol content in tobacco.

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