Image-sensor-based detection system of colloidal gold immunoassay

An image sensor and immune analysis technology, which is applied to the analysis of materials, material analysis by observing the influence of chemical indicators, and color measurement using electric radiation detectors. It can solve the problem of accurately collecting image data at a speed of seconds The processing speed cannot meet the problems of dynamic tracking and observation of the reaction process, and achieves the effect of large dynamic range, meeting the requirements of dynamic tracking and observation of the reaction process, and fast acquisition speed

Inactive Publication Date: 2014-05-21
SHANGHAI YAOYAN PHOTOELECTRIC SCI & TECH
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Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a colloidal gold immunoassay detection system based on an image sensor, which can solve the problem that the exis

Method used

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  • Image-sensor-based detection system of colloidal gold immunoassay
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Embodiment

[0072] Such as figure 1 As shown, the lighting source 201 in the lighting module 2 adopts 8 light-emitting diodes with a wavelength of 525nm, a divergence angle of 180 degrees, a light intensity of 500mcd, and a diameter of 3mm, arranged in a circular array, and then shading treatment is performed on each diode , so that it only outputs light in the radial direction, and then the light-emitting diodes are symmetrically arranged in a ring around the optical axis of the imaging lens group. The light homogenizing unit 202 adopts a homogenizing glass plate, one side of which is coated with an anti-reflection film, and the center wavelength is 537nm, so that the light can be fully transmitted, and the other side is roughened to fully scatter, and it is placed under the light emitting diode array 201, so in Light with uniform brightness and intensity can be obtained on the test strip 102 .

[0073] The CMOS image sensor 301 of the imaging module 3 can adopt the OV528 serial interfa...

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Abstract

The invention discloses an image-sensor-based detection system of colloidal gold immunoassay and belongs to the field of the colloidal gold immunoassay. The detection system is as follows: a test strip placing platform for placing a test strip is arranged on a test strip detection platform; an illumination module and an imaging module are arranged above the test strip placing platform of the test strip detection platform; the illumination module consists of an illumination light source and a light homogenization unit arranged below the illumination light source; the illumination light source is a hollow annular light-emitting diode array light source formed by means that a plurality of light-emitting diodes are uniformly formed in an annular array; an image sensor of the imaging module uses a CMOS image sensor and the signal output end of the imaging module is electrically connected with the input end of a data collecting and processing device. The system adopts the annular matrix illumination and is provided with the light homogenization unit so that output light is homogenized and the detection result is accurate; the imaging module adopts the CMOS image sensor, so that the collection speed is high, and the requirement of dynamically tracking and observing the reaction process can be met.

Description

technical field [0001] The invention relates to the field of colloidal gold immunoassay detection instruments, in particular to an image sensor-based instrument for colloidal gold immunoassay, which can be used for quantitative detection of colloidal gold immunoassays in the fields of biological detection technology, food safety detection and the like. Background technique [0002] Colloidal gold can be used as a marker for immunohistochemical reaction by utilizing the characteristic that colloidal gold can be closely combined with protein through electrostatic. It has been valued by related research fields, and the colloidal gold detection technology derived from it has also been developed accordingly. In recent years, various domestic research institutes and companies have devoted themselves to the research of colloidal gold detection instruments. [0003] Colloidal gold detectors produced on the market in recent years have no more than two methods for collecting and proc...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N21/78G01N21/31
CPCG01N33/558F21V5/08F21V19/001F21Y2101/00G01J3/50
Inventor 王建辉曹晓君
Owner SHANGHAI YAOYAN PHOTOELECTRIC SCI & TECH
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