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A kind of preparation method and application of hematoxylin

A technology of hematoxylin and hematoxylin, which is applied in the field of medicine, can solve the problems of stopping and not seen, and achieves the effects of simple method, enhanced immunity and easy operation.

Inactive Publication Date: 2016-03-02
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on the traditional Chinese medicine hematoxylin still stops at its high isoflavones, phenolic components, brazilian hematoxylin, prohematoxylin, chalcones, flavonols, sterols, fatty acids and amino acids, etc. Polysaccharides in plants have immunomodulatory, anti-virus, anti-cancer, anti-oxidation, hypoglycemic and other activities in clinical applications, and have no toxic side effects on normal cells, so it is important for the activity of polysaccharides in various medicinal plants Reports are common, but so far there is no research on the composition and application of polysaccharides in sumac

Method used

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  • A kind of preparation method and application of hematoxylin
  • A kind of preparation method and application of hematoxylin
  • A kind of preparation method and application of hematoxylin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] In the specific implementation of the present invention, get the hematoxylin medicinal material, add water with 6 times the weight of the medicinal material, soak for 30min, place in a flash extractor and extract for 1min, filter to obtain the filtrate for the first time; Soak in water for 5 minutes, filter to obtain the second filtrate, combine the two filtrates, concentrate in vacuum to 5 times the weight of hematoxylin, and centrifuge at 4000-6000 rpm for 15 minutes to obtain the supernatant, which is the hematoxylin crude polysaccharide liquid; Take papain, use Na at pH5.0-7.0 2 HPO 4 -NaH 2 PO 4 Dissolve the buffer solution to make a papain solution with a concentration of 0.6%; mix the hematoxylin crude polysaccharide solution and the papain solution at a ratio of 1:0.3, and bathe in a water bath at 35°C for 8 hours to obtain an enzymatic hydrolysis solution. Deproteinization, polysaccharide solution, polysaccharide solution decompression recovery solvent to 1....

Embodiment 2

[0011] In the present invention, take hematoxylin medicinal material, add water with 8 times the weight of the medicinal material, soak for 30 minutes, place in a flash extractor to extract for 2 minutes, and filter to obtain the filtrate for the first time; add the dregs of the medicinal material and soak in water with 8 times the weight of the original medicinal material for 7 minutes , filter to get the second filtrate, combine the two filtrates, concentrate in vacuum to 7 times the weight of hematoxylin, and centrifuge at 4000-6000 rpm for 15 minutes to get the supernatant, which is the crude polysaccharide solution of hematoxylin; take papain , with Na at pH5.0-7.0 2 HPO 4 -NaH 2 PO 4 Dissolve the buffer solution to make a papain solution with a concentration of 0.8%; mix the hematoxylin crude polysaccharide solution and the papain solution at a ratio of 1:0.45, bathe in water at 45-50°C for 6 hours, and obtain the enzymatic hydrolysis solution, and put the enzymatic hy...

Embodiment 3

[0013] Take sumac medicinal material, add water 10 times the weight of the medicinal material, soak for 30 minutes, place it in a flash extractor for extraction for 3 minutes, filter to obtain the first filtrate; For the second filtrate, combine the two filtrates, concentrate in vacuum to 8 times the weight of hematoxylin, and centrifuge at 4000-6000 rpm for 15 minutes to obtain the supernatant, which is the crude polysaccharide solution of hematoxylin; get papain, and use pH5. 0-7.0Na 2 HPO 4 -NaH 2 PO 4 Dissolve the buffer solution to make a papain solution with a concentration of 1.0%; mix the hematoxylin crude polysaccharide solution and the papain solution at a ratio of 1:0.6, and bathe in a water bath at 60°C for 3 hours to obtain an enzymatic hydrolysis solution. Deproteinization, polysaccharide solution, polysaccharide solution decompression recovery solvent to 2.5 times the weight of the hematoxylin medicinal material, to obtain a concentrated solution, the concent...

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Abstract

The invention relates to a preparation method and application of hematoxylon polysaccharide, which effectively solves the problems about preparation of hematoxylon polysaccharide and application of hematoxylon polysaccharide in preparation of immunity-enhancing medicines and health-care products. The method comprises the following steps: soaking hematoxylon medicinal material with water, and extracting to obtain a first filtrate; soaking the dregs with, and filtering to obtain a second filtrate, mixing the filtrates, concentrating in vacuum and centrifuging to obtain supernate, namely hematoxylon coarse polysaccharide liquid; dissolving papain by using a Na2HPO4-NaH2PO4 buffer liquid to prepare a papain liquid; mixing the hematoxylon coarse polysaccharide liquid and the papain liquid, carrying out water bath, cooling to room temperature naturally, and deproteinizing to obtain polysaccharide liquid; recycling the solvent from the polysaccharide liquid under reduced pressure to obtain concentrated liquor; adding ethanol into the concentrated liquor, standing still, and filtering out the liquid at the upper part to obtain a precipitate; and sequentially washing the precipitate by using ethanol, acetone and absolute ether, and drying under reduced pressure to obtain the hematoxylon polysaccharide. The method provided by the invention is simple and easy to operate, and can be effectively used for preparing hematoxylon polysaccharide. The hematoxylon polysaccharide can be used for preparing immunity-enhancing medicines and health-care products.

Description

technical field [0001] The invention relates to medicine, in particular to a preparation method and application of hematoxylin polysaccharide. Background technique [0002] Dried heartwood of leguminous plant Caesalpiniasappan L., sweet, salty, flat, Guixin, liver, spleen meridian, has the effect of promoting blood circulation, removing blood stasis, reducing swelling and relieving pain, used for bruises, fractures, tendon injuries, stasis, swelling and pain , amenorrhea dysmenorrhea, postpartum stasis, chest and abdomen tingling, carbuncle swelling and pain. At present, the research on the traditional Chinese medicine hematoxylin still stops at its high isoflavones, phenolic components, brazilian hematoxylin, prohematoxylin, chalcones, flavonols, sterols, fatty acids and amino acids, etc. Polysaccharides in plants have immunomodulatory, anti-virus, anti-cancer, anti-oxidation, hypoglycemic and other activities in clinical applications, and have no toxic side effects on nor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61P37/04
Inventor 冯素香刘富岗周悌强吴兆宇李晓玉王蒙蒙
Owner HENAN UNIV OF CHINESE MEDICINE
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