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Stable soybean hundred-grain weight related QTL (quantitative trait loci) locus and close linkage mark satt281 thereof

A 100-kernel weight and soybean technology, which is applied in the field of soybean 100-kernel weight-related QTL loci and its closely linked marker satt281, can solve the problems of being susceptible to environmental factors, low breeding efficiency, and the like, and improve breeding efficiency and soybean yield. , the effect of shortening the breeding cycle

Inactive Publication Date: 2014-07-02
INST OF CEREAL & OIL CROPS HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to detect and locate a stable soybean 100-seed weight-related QTL site and closely linked marker satt281 through research and detection, so as to overcome the problem of low breeding efficiency in conventional breeding due to phenotypic selection being easily disturbed by environmental factors

Method used

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  • Stable soybean hundred-grain weight related QTL (quantitative trait loci) locus and close linkage mark satt281 thereof
  • Stable soybean hundred-grain weight related QTL (quantitative trait loci) locus and close linkage mark satt281 thereof
  • Stable soybean hundred-grain weight related QTL (quantitative trait loci) locus and close linkage mark satt281 thereof

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Embodiment

[0015] Two-generation recombinant inbred lines (RIL) of Jidou 12×Heidou combination F8 and F9, including 216 families, were used as materials. Jidou 12 is a variety bred by Hebei Province Grain and Oil Institute, with a hundred-grain size of 21g; black soybean is a semi-wild material from farmers. The two parents were quite different in geographical origin, genetic origin and agronomic traits. In the summer of 2011, F8RIL and the parental design random block group 3 were repeatedly sown in Shijiazhuang Embankment Experimental Station, with a row length of 3m and a row spacing of 0.5m; Sow F9RIL and its parents in Shijiazhuang Dishang Experimental Station with the same row spacing as in 2011. After the material matures naturally, it is harvested and dried in the sun. According to the standards in the "Soybean Germplasm Resources Description Specifications and Data Standards", the 100-grain weight is measured, and 100 complete seeds are weighed and counted with a percent balanc...

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Abstract

The invention relates to a stable soybean hundred-grain weight related QTL (quantitative trait loci) locus and a close linkage mark satt281 thereof, which belong to the technical field of crop selective breeding. The locus is located on a linkage group of soybean C2, the close linkage mark is an SSR primer satt 281 and located on a position of 40.3cm on a public map, and the close linkage mark satt281 can be detected in two generations and two environments by adopting four different location methods (double-tail and single marking square difference analysis adopts the P which is less than or equal to 0.01 as the standard, and CI and MICIM adopt LOD which is more than or equal to 3 as the standard). The trait related QTL is located by adequately utilizing different methods, the hundred-grain weight QTL detection situations are compared in different environments and different generations, and the stable soybean hundred-grain weight QTL locus can be determined. The stable soybean hundred-grain weight related QTL can be used for improving the hundred-grain weight traits by utilizing the molecular mark auxiliary selection and map-based cloning, so that powerful technical support can be provided for the breeding and improvement of the soybean crops.

Description

technical field [0001] The invention relates to the technical field of crop seed selection and breeding, in particular to a stable soybean hundred-grain weight-related QTL locus and its closely linked marker satt281. Background technique [0002] Soybean is an important oil crop and high-protein crop, and it is also one of the five major crops in my country. As the country of origin of soybeans, my country was once the largest soybean producer and exporter in the world. However, due to the slow growth of soybean yield per unit area, my country's soybean planting area and production have continued to decline in the past ten years. With the increasing domestic demand for soybeans, my country has become one of the largest soybean importing countries in the world. Compared with other high-yield crops, the relative yield of soybean is relatively low at present. Improving the yield potential of soybean is an important task of soybean breeding. High-yield breeding has always been...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 张孟臣陈强闫龙
Owner INST OF CEREAL & OIL CROPS HEBEI ACAD OF AGRI & FORESTRY SCI
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