Jujube polysaccharide, extraction and purification method and its application as tobacco humectant
A jujube polysaccharide and humectant technology, which is applied in the fields of tobacco, application, and tobacco treatment, to achieve the effects of reducing impurities, obvious moisturizing effect, and broad application prospects
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Embodiment 1
[0018] A method for extracting and purifying jujube polysaccharides, comprising the steps of: extracting 300 g of crushed dried jujube with 1800 ml of ether Soxhlet for 2 hours to remove oil, and evaporating the residue to dryness. The residue after evaporating to dryness was extracted three times with 750ml boiling water, combined the boiling water extracts obtained three times, concentrated, centrifuged, and the supernatant was taken, and the supernatant was added with absolute ethanol 3 times the volume of the clear liquid for alcohol precipitation, and stood overnight (12h), again. The precipitate was washed three times with 300ml ethanol and 300ml acetone in turn, and then dissolved in distilled water, then papain with a weight of 1‰ of the precipitate was added and placed in a water bath at 50°C for 2 hours, and the protein was removed by Sevage method. Add 8g of activated carbon and place it in a 70°C water bath to heat for 3 hours, remove the activated carbon by filtra...
Embodiment 2
[0021] A method for extracting and purifying jujube polysaccharides, comprising the following steps: 300 g of crushed dried jujube is extracted with 2000 ml of ether Soxhlet for 4 hours to remove oil, and the residue is evaporated to dryness. The residue after evaporating to dryness was extracted with 750ml of boiling water for 3 times, and the boiling water extracts obtained three times were combined, concentrated, centrifuged, and the supernatant was taken, and anhydrous ethanol with 3 times the volume of the clear liquid was added for alcohol precipitation, and stood for 12 hours , and centrifuge again to collect the precipitate. The precipitate was washed 3 times with 300ml ethanol and 300ml acetone in turn, and then dissolved in distilled water, then papain with a weight of 2‰ of the precipitate was added and placed in a water bath at 50°C for 4 hours, and the protein was removed by the Sevage method. Add 8g of activated carbon and place it in a 70°C water bath to heat fo...
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