New application of 5-HT3AR inhibitor Ondansetron

A 1.5-HT3AR, 2.5-HT3AR technology, applied in the field of 5-HT3AR inhibitor Ondansetron, can solve the problem of not being able to effectively simulate all symptoms of clinical pain

Inactive Publication Date: 2014-08-20
SHANGHAI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, none of the above models can effectively simulate all the symptoms of clinical pain. Therefore, finding new natural stimuli, more accurately and effectively simulating clinical pain symptoms, and establishing animal models that simultaneously present clinical persistent spontaneous pain and hyperalgesia-related reactions have become pain research. urgent need

Method used

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  • New application of 5-HT3AR inhibitor Ondansetron
  • New application of 5-HT3AR inhibitor Ondansetron
  • New application of 5-HT3AR inhibitor Ondansetron

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Observation of Different Doses of Ondansetron Relieving Rats' Spontaneous Paw Withdrawal and Painful Grand Attack Response

[0018] Put a 75 cm high bracket on the table covered with a glass mirror, and place a 20×20×30cm 3 Transparent plexiglass box, from the mirror on the table to observe the reaction of rat feet after administration. Before the drug injection, the rats were placed in the box 30 minutes in advance to adapt to the experimental environment. After the plantar injection of BmK I, the animals were put back into the detection box, and the spontaneous pain behavior was continuously observed. The nociceptive spontaneous pain response was evaluated by the number of times of spontaneous foot lifting every 5 minutes, the number of screams and other great pain attacks. The way of administration of Ondansetron is to inject 10ul of 5ug / ul or 10ug / ul intrathecally into rats 30 minutes before BmK I injection. Ondansetron's relief of spontaneous pain a...

Embodiment 2

[0020] Example 2: Observation of Different Doses of Ondansetron Alleviating Bilateral Mechanical Hyperalgesia in Rats

example 1

[0021] After the spontaneous pain disappeared substantially in Example 1, the rats were placed on a steel wire grid (1cm 2 ) on a plexiglass box (20×20×30 cm 3 )middle. A series of Von Frey fibers (58011, Stoelting Co., USA) (from 0.6 to 26 g) were used to measure the mechanical withdrawal reflex threshold. It can be measured when the rat's injected hind foot touches the ground and bears normal weight. Stimulate the skin of the plantar center of the hindlimb of the animal on the injection side from under the grid, bend each fiber to an appropriate degree and stay for 2-3 seconds, and the interval between two measurements is 10 seconds. The stimulation intensity of Von Frey fibers ranged from small to large. If there is no withdrawal reflex, continue with a larger grammage of fiber, up to a maximum of 26 g. The minimum gram value that can cause 50% paw withdrawal response was taken as the mechanical paw withdrawal reflex threshold. The mechanical pain threshold was collect...

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Abstract

The invention relates to a new application of a 5-HT3AR inhibitor Ondansetron. According to the invention, different doses of Ondansetron are injected into a mouse intrathecal L5-L6 sacral space 30 minutes before BmKI injection, and relief from experimental spontaneous pain, paroxysmal pain with grand mal, bilateral mechanical hyperalgesia, unilateral thermal hyperalgesia and the like with the use of different doses of Ondansetron is studied. The invention is based on an experimental animal inflammatory pain model, providing a foundation for research of an analgesia molecular mechanism related to a 5-HT3AR signal pathway and research and development of a novel analgesic.

Description

technical field [0001] The invention relates to a new application of Ondansetron, a 5-HT3AR inhibitor. Background technique [0002] Pain can be broadly divided into acute pain and chronic pain. The former is an indispensable protective mechanism of the body, while the latter is caused by various etiologies such as trauma, infection, inflammation, and tumors. Because of its long course of disease, slow recovery, and complicated pathogenesis, it has become an important cause of endangering people's health. . Due to the lack of effective clinical relief strategies for chronic pain, existing drugs such as morphine and non-steroidal anti-inflammatory drugs have problems such as poor analgesic effect, addiction, and toxic side effects. Therefore, elucidating its molecular mechanism and finding effective analgesic strategies have become the focus in the field of life medicine. [0003] In order to simulate the clinical symptoms of human chronic pain, researchers have establishe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4178A61P29/00
Inventor 吉永华傅晋焦云露李正威杨清湖
Owner SHANGHAI UNIV
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