Artemisia stalk-decomposing bacterial agent for inhibiting artemisia soil-borne pathogens and preparation method thereof
A technology of straw decomposing bacteria and pathogenic bacteria, applied in the field of microorganisms, can solve the problem of not finding literature reports, etc., and achieve the effects of inhibiting growth and reproduction, increasing the decomposition rate, and being beneficial to safe production.
Active Publication Date: 2016-01-06
江苏农丰宝生物科技有限公司
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[0007] So far, no literature reports related to the subject of the present invention have been found
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[0029] Below in conjunction with embodiment the present invention will be further described.
[0030] (1) Acquisition of functional microbial strains
[0031] In the continuous cropping field of Artemisia annua, select the best-growing plants in the field with high incidence rate, collect the rhizosphere soil of the plant, store it at low temperature, use Martin's medium to isolate fungi, beef extract peptone medium to isolate bacteria, Gaoshi No. 1 Culture medium to isolate actinomycetes.
[0032] The above-mentioned Martin’s medium preparation method is (take the preparation of 1L medium as an example): 5g of peptone, 10g of glucose, KH 2 PO 4 1g, MgSO 4 0.5g, 20g agar, 1000ml water, natural pH, 3.3ml 1% Bengal red aqueous solution, sterilize at 115°C for 30min, add 0.3ml 1% streptomycin solution per 100ml medium before use. The preparation method of the above-mentioned beef extract-peptone medium is as follows (take the preparation of 1L medium as an example): 3g of b...
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The invention discloses an Artemisia stalk-decomposing bacterial agent for inhibiting Artemisia soil-borne pathogenic bacteria and a preparation method thereof. Bacillus subtilis strain and Trichoderma reesei strain produce artemisia stalk decomposing bacteria agent. The bacterial agent can accelerate the decomposition speed of the Artemisia stalk residue in the Artemisia continuous cropping soil, inhibit the reproduction and proliferation of pathogenic microorganisms on the straw residue, and reduce the harm of the Artemisia continuous cropping obstacle. The number of functional microorganisms in the bacterial agent≧2×10 8 cfu•g -1 , the water content is less than 15%, and the organic matter content is greater than 35%. The test shows that in the land with serious occurrence of Artemisia continuous cropping obstacles, after applying the decomposing bacteria agent of Artemisia straw residues, compared with the soil without decomposing bacteria agents, the weight loss rate of Artemisia straw decomposition increased by 80.9%, and the incidence of Artemisia stalks in the next crop decreased. 75%, and the yield of Artemisia annua increased by 116%. The straw decomposing bacteria agent can well inhibit the continuous cropping diseases of Artemisia annua.
Description
Technical field [0001] The present invention involves an artemodium straw decomposed bacteria and their preparation methods that suppress the original bacteria of the soil transmission, which belongs to the field of microbial technology. Background technique [0002] Artemisia Artemisia Artemisiaselengens Turcz.) Also known as Artemisia Artemisia, Artemisia annua, Artemisia, Artemisia annua, Xiang Ai, and narrow leaf Ai, it is a perennial herbaceous plant.An Artemisia is one of the most popular vegetables for fresh and tender stems. It is one of the main wild vegetable varieties supplied by winter and spring vegetable markets in my country. [0003] With the development of intensive production of artemisia, planting one acre of artemisia can increase their income tens of thousands of yuan. Some townships and villages are vegetable planting bases. The planting area of Artemisia annua accounts for about 60%of the local arable land.The long -term planting of the soil is likely to ca...
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IPC IPC(8): A01N63/04A01P1/00A01P3/00A01N63/02
Inventor 陈立华常义军王长春张营唐丹翟亚明谈俊益
Owner 江苏农丰宝生物科技有限公司