Non-tumorigenic mdck cell line and screening method for amplifying influenza virus

An influenza virus, non-tumorigenic technology, applied in microorganism-based methods, animal cells, viruses/phages, etc., can solve the problems of non-tumorigenicity, 1-100 cells may form tumors, etc., and achieve cost savings , the effect of shortening the time
CN104059873BActive Publication Date: 2016-09-28SHANGHAI INST OF BIOLOGICAL PROD CO LTD
3 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHANGHAI INST OF BIOLOGICAL PROD CO LTD
Publication Date
2016-09-28

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a non-tumor-causing MDCK cell line for amplifying influenza virus and a screening method thereof. The cell line is MDCK‑2B2, and the preservation number is CCTCC C201323. The present invention provides a non-tumorigenic cell line MDCK-2B2 and its subclone cell line, which grow into adherent cells and / or have epithelial-like morphology and / or can support the replication of various viruses, and can amplify Higher titer virus; In addition, the present invention has found a method for screening non-tumorigenic cell lines, which can assist the judgment of tumorigenicity through the growth rate of cells and the morphological changes of cells, that is, relatively slow-growing single-pore cells, which The tumorigenicity will be significantly reduced, and the appearance of giant cells will significantly increase the tumorigenicity. When screening non-tumorigenic cell lines on a large scale, such auxiliary judgment methods can shorten time and save costs.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The present invention relates to MDCK-derived cell lines, more specifically, to a non-tumorigenic MDCK cell line for amplifying influenza virus and a screening method thereof. Background technique

[0002] The annual influenza epidemic will have a relatively large impact on both developed and developing countries. According to statistics, in the season when influenza is prevalent, about 5-15% of the people in the world will develop upper respiratory tract infection every year, and about 250,000-500,000 people will die [WHO. Influenza Fact Sheet www.who.int / mediacentre / factsheets / fs211 / en / ]. Influenza vaccination is one of the main measures to prevent and control influenza, which can significantly reduce morbidity and mortality, so it is particularly important to provide vaccines effectively and quickly. At present, most influenza vaccines still use chicken embryos to produce the strains announced by WHO. Due to the shortage of chicken embryos in the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More