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Quantitative gastrin-releasing peptide precursor kit, as well as preparation method and detection method thereof

A technology for quantitative determination of gastrin-releasing peptides, which is applied in measurement devices, instruments, scientific instruments, etc., to achieve the effect of strong specificity and high sensitivity

Inactive Publication Date: 2014-10-08
JIANGSU FLON BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this patent does not involve magnetic particles and will not affect our company's patent application for this invention

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  • Quantitative gastrin-releasing peptide precursor kit, as well as preparation method and detection method thereof
  • Quantitative gastrin-releasing peptide precursor kit, as well as preparation method and detection method thereof
  • Quantitative gastrin-releasing peptide precursor kit, as well as preparation method and detection method thereof

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Experimental program
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Embodiment Construction

[0086] see Figure 1-Figure 3 , the gastrin-releasing peptide precursor (ProGRP) quantitative assay kit of the present invention comprises a box body 1 and a box cover 2 above the box body, the box body 1 is provided with a microwell plate 6 and a box located at the bottom of the box body 1 A support plate 3 made of foam plastics, wherein the microporous plate 6 is composed of 48 or 96 microholes 7, a plurality of holes 4 are opened on the support plate 3, and eight reagent bottles 5 are placed in the holes 4, The eight reagent bottles 5 respectively contain magnetic separation reagents, enzyme reactants, stability enhancers, calibration products, quality control products, cleaning concentrates, diluents and substrate solutions, wherein the magnetic separation reagents contain gastrin releasing peptide Precursor (ProGRP) monoclonal antibody coated magnetic particles.

[0087] Preparation

[0088] Step 1, Preparation of Magnetic Separation Reagent

[0089] 1....

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Abstract

The invention relates to a quantitative gastrin-releasing peptide precursor (ProGRP) kit and a detection method thereof. A method comprises seven steps of preparing a magnetic separation reagent, preparing an enzyme reactant, preparing a reaction enhancer, preparing a calibration substance diluent, preparing a calibration substance and a quality control substance, preparing a cleaning concentrate liquid and preparing a substrate solution. According to the quantitative gastrin-releasing peptide precursor kit and the detection method thereof, the sensitivity and the specificity are relatively high, the detection result obtaining time is relatively short, the operation mode is relatively simple, cell lung cancers (SCLC) and non small cell lung cancer (NSCLC) can be distinguished, and the kit has the important significance on early diagnosis on lung cancers.

Description

technical field [0001] The present invention relates to a gastrin-releasing peptide precursor (ProGRP) quantitative assay kit, its preparation method and detection method. The quantitative assay kit is widely used in the early detection and detection of various lung cancers including small cell lung cancer. Treatment monitoring, recurrence monitoring, etc. Background technique [0002] Due to the influence of factors such as population aging, urban industrialization, smoking, air and environmental pollution, and genetic susceptibility, the incidence and mortality of lung cancer in my country have risen rapidly. According to the statistical data of the 2012 Tumor Registration Annual Report, lung cancer has replaced liver cancer as the first cause of death from malignant tumors in my country. Currently, 1 out of every 4 deaths from malignant tumors in my country is caused by lung cancer. [0003] Lung cancer is mainly divided into small cell lung cancer (SCLC) and no...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N33/574G01N33/577G01N33/531
Inventor 奚伟红朱丹丹王京高淑舫陈美
Owner JIANGSU FLON BIOTECH
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