Broad-spectrum antibacterial saccharomycete

A yeast, broad-spectrum technology, applied in the field of yeast, can solve the problems of high price, damage to organs, incomplete cleaning, etc., and achieve the effect of significant bacteriostatic effect, simple nutritional requirements and fast reproduction.

Active Publication Date: 2014-11-19
上海艾昂生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although chemically synthesized preservatives can prolong the shelf life of fruits and vegetables to a certain extent, and have a significant antibacterial effect; but if they are not cleaned thoroughly, they are easily eaten and absorbed by the human body. These chemical preservatives entering the body will damage the internal organs of the human body and affect human health.
At present, the natural preservatives that are non-toxic and harmless to the human body are rare and expensive, and are not suitable for mass consumer goods.

Method used

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  • Broad-spectrum antibacterial saccharomycete
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  • Broad-spectrum antibacterial saccharomycete

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0029] Embodiment 1: The broad-spectrum antibacterial yeast in this embodiment is Torulaspora delbrueckii M9, which is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 2013278.

[0030] Torulaspora delbrueckii M9 was collected from Tibet and isolated from Tibetan Linggu, a traditional fermented dairy product in my country. The Tibetan Linggu was subcultured in 10% skim milk, and after activation, the fermented liquid was spread on the YEPD solid medium containing chloramphenicol and streaked to obtain Torulaspora delbrueckii M9.

[0031] The base length of the 26s rDNA D1 / D2 region of Torulaspora delbrueckii M9 is 566 bp, the base sequence is shown in SEQ ID NO: 2, the 18S rDNA length is 1657 bp, and the base sequence is shown in SEQ ID NO: Shown in 1; after comparing the 26S rDNA D1 / D2 region sequence with the 18S rDNA sequence, and through the identification of microbial morphology and physiological and biochemical indicat...

Embodiment 1

[0043] Embodiment 1 antibacterial experiment:

[0044] Make antibacterial test plate: Pour 8mL plain agar into the sterilized plate, put an Oxford cup on the agar after solidification; add Shigella, Candida albicans, Escherichia coli, Salmonella, Bacillus subtilis, golden Add 4mL of normal saline to the slant medium of Staphylococcus aureus, then shake it, then take 0.5mL from it and inject it into 4.5mL of sterile normal saline, shake it well, repeat the above gradient dilution 3 times, and prepare 10 -3 Bacterial suspension; use a vortex oscillator to shake the bacterial suspension evenly, then take 1mL and add it to 25mL nutrient medium, mix well and pour it into the above-mentioned agar plate to be used, pull out the Oxford cup after the agar solidifies, and put Add 100 μL of concentrated fermentation broth of Torulaspora delbrueckii M9 to the wells (add 100 μL of Nisin solution to the remaining wells to complete the Nisin comparison) to make a bacteriostatic test plate; ...

Embodiment 2

[0054] ①Concentrated fermentation broth of Torulaspora delbrueckii M9: Torulaspora delbrueckii M9 was fermented and cultured with YEPD liquid medium, the fermentation time was 12 hours, and the fermentation temperature was 28°C; then The Torulaspora delbrueckii M9 fermentation broth was centrifuged at 4000r / min for 10min, and the supernatant was concentrated in a rotary evaporator at a vacuum of 660mmHg and 65°C to 1 / 4 of the original volume, 1 / 8 and 1 / 16, the concentrated fermentation liquid of Torulaspora delbrueckii M9 was obtained; then the concentrated fermentation liquid of Torulaspora delbrueckii M9 was put into Heating in a water bath at 80°C, 90°C and 100°C for 20 minutes;

[0055] The YEPD liquid medium is composed of 1 g of yeast extract, 2 g of peptone, 2 g of glucose and 100 mL of distilled water in proportion, and the pH value of the YEPD liquid medium is 5.5.

[0056] Pour 8mL of plain agar into the sterilized plate, put an Oxford cup on the agar after solidifi...

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Abstract

The invention discloses broad-spectrum antibacterial saccharomycete and relates to saccharomycete. The broad-spectrum antibacterial saccharomycete is torulaspora delbrueckii M9, collected in China Center for Type Culture Collection (CCTCC), along with a collection number of CCTCC NO: M 2013278. The Torulaspora delbrueckii M9 has stable and excellent antibacterial effects on shigella, candida sporogenes, escherichia coli, salmonella, bacillus subtilis and staphylococcus aureus; the broad-spectrum antibacterial saccharomycete is applied in the field of microorganisms.

Description

technical field [0001] The present invention relates to a yeast. Background technique [0002] Yeast is a single-celled microorganism belonging to the fungus class of higher microorganisms, which can survive in both aerobic and anaerobic environments, and belongs to facultative anaerobic bacteria. Yeast is the most directly consumed microorganism by humans and is currently used to ferment food. [0003] In food, fruits and vegetables are difficult to keep fresh, the shelf life is short, and the surface is easily infected with pathogenic bacteria. In order to keep fruits and vegetables fresher, chemically synthesized preservatives are currently used for preservation. Although chemically synthesized preservatives can prolong the shelf life of fruits and vegetables to a certain extent, and have a significant antibacterial effect; but if they are not cleaned thoroughly, they are easily eaten and absorbed by the human body. These chemical preservatives entering the body will da...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12R1/645
Inventor 孟利刘峰韩德权史玲玲李鹏飞
Owner 上海艾昂生物技术有限公司
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