Kit for detecting common depletion alpha-thalassemia and use method thereof

A technology of thalassemia and reagent kit, which is applied in the field of medical detection, and can solve problems such as long section length and long detection time
CN104178573AInactive Publication Date: 2014-12-03龙驹
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
龙驹
Publication Date
2014-12-03
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a kit for detecting common deletion form alpha-thalassemia and a using method of the kit. The kit comprises a pair of primers capable of performing simultaneous amplification of a signature sequence A1 in an alpha 1 section and a signature sequence A2 in an alpha 2 section in an alpha-globin gene cluster, a pair of primers capable of performing amplification of a --<SEA> genetype in the alpha-globin gene cluster and a pair of primers capable of performing amplification of a --<THAI> genetype in the alpha-globin gene cluster as well as a fluorescence probe for specific detection of the signature sequence A1 in the alpha 1 section, a fluorescence probe for specific detection of the signature sequence A2 in the alpha 2 section, a fluorescence probe for specific detection of a --<SEA> genetype amplicon and a fluorescence probe for specific detection of a --<THAI> genetype amplicon. As for the alpha-thalassemia globin gene deletion detection, the kit provided by the invention has high sensitivity, stability, accuracy and specificity.
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Description

technical field

[0001] The invention relates to the technical field of medical detection, in particular to a kit for detecting common deletion α-thalassemia and a use method thereof. Background technique

[0002] Thalassemia, referred to as thalassemia, is one of the most common monogenic genetic diseases. The common types of thalassemia are α-thalassemia and β-thalassemia, which are caused by abnormal expression of α-protein gene cluster and β-globin gene cluster respectively. . In my country, Guangxi, Guangdong and Hainan are provinces with high incidence of thalassemia, and the incidence rate of α-thalassemia in Guangxi is 15.5%. In the Chinese population, the common genotype of the deletion α-thalassemia is -- SEA , -α 3.7 and-alpha 4.2 , and the relatively common Thai-type deletion in Guangxi (-- THAI ).

[0003] Homologous gene quantitative technology is a method based on gene homology, using common amplification primers to amplify homologous genes, and finally u...

Claims

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