Kit for detecting common depletion alpha-thalassemia and use method thereof

Abstract

The invention discloses a kit for detecting common deletion form alpha-thalassemia and a using method of the kit. The kit comprises a pair of primers capable of performing simultaneous amplification of a signature sequence A1 in an alpha 1 section and a signature sequence A2 in an alpha 2 section in an alpha-globin gene cluster, a pair of primers capable of performing amplification of a --<SEA> genetype in the alpha-globin gene cluster and a pair of primers capable of performing amplification of a --<THAI> genetype in the alpha-globin gene cluster as well as a fluorescence probe for specific detection of the signature sequence A1 in the alpha 1 section, a fluorescence probe for specific detection of the signature sequence A2 in the alpha 2 section, a fluorescence probe for specific detection of a --<SEA> genetype amplicon and a fluorescence probe for specific detection of a --<THAI> genetype amplicon. As for the alpha-thalassemia globin gene deletion detection, the kit provided by the invention has high sensitivity, stability, accuracy and specificity.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a kit for detecting common deletion α-thalassemia and a use method thereof. Background technique [0002] Thalassemia, referred to as thalassemia, is one of the most common monogenic genetic diseases. The common types of thalassemia are α-thalassemia and β-thalassemia, which are caused by abnormal expression of α-protein gene cluster and β-globin gene cluster respectively. . In my country, Guangxi, Guangdong and Hainan are provinces with high incidence of thalassemia, and the incidence rate of α-thalassemia in Guangxi is 15.5%. In the Chinese population, the common genotype of the deletion α-thalassemia is -- SEA , -α 3.7 and-alpha 4.2 , and the relatively common Thai-type deletion in Guangxi (-- THAI ). [0003] Homologous gene quantitative technology is a method based on gene homology, using common amplification primers to amplify homologous genes, and finally u...

AI Technical Summary

Problems solved by technology

However, due to -α 3.7 and-alpha 4.2 All are produced by the recombination of large segment homologous genes, so the -α carried by different individuals 3.7 or -alpha 4.2 The gene sequence is not consistent, therefore, the current commonly used diagnostic method is to amplify it by spanning breakpoint PCR (Gap-PCR), but because of the long segment length, the detection time is also long

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