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Rapid propagation and cultivation method of mangosteen

A cultivation method and technology of mangosteen, applied in the field of plant tissue culture, can solve the problems that it is difficult to realize large-scale breeding of mangosteen seedlings, low breeding coefficient, and small number of bud points, etc., and achieve good growth, stable breeding, and improved reproduction speed effect

Active Publication Date: 2014-12-24
HAIKOU EXPERIMENTAL STATION CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although cutting branches are easy to root, small seedlings have fewer buds and a very low reproduction coefficient, making it difficult to achieve large-scale breeding of mangosteen seedlings

Method used

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  • Rapid propagation and cultivation method of mangosteen
  • Rapid propagation and cultivation method of mangosteen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A kind of mangosteen rapid multiplication culture method, comprises the following steps:

[0028] (1) Surface sterilization of explants

[0029] Mangosteen seeds obtained from mature mangosteen fruits, washed 1-2 times with an aqueous solution containing 1% washing powder or detergent in mass concentration or rinsed with running water for 1-5 hours; then dipped in alcohol with a volume fraction of 65% for 5-15s , shake the mangosteen seeds while dipping, rinse 2-4 times with sterile water; finally soak them in the disinfectant solution with a volume concentration of 5% sodium hypochlorite and a volume concentration of 0.1% Tween 80 for 15-20min, shake the mangosteen seeds while soaking, without Rinse with bacterial water for 3-5 times, and the mangosteen seeds after surface disinfection and sterilization are ready for use; in order to avoid the reduction of the activity of mangosteen seeds, the seeds taken from the fruit must be processed within 5 days;

[0030] (2) In...

Embodiment 2

[0036] A kind of mangosteen rapid multiplication culture method, comprises the following steps:

[0037] (1) Surface sterilization of explants

[0038] Mangosteen seeds obtained from mature mangosteen fruits, washed 1-2 times with an aqueous solution containing 2% washing powder or detergent in mass concentration or rinsed with running water for 1-5 hours; then dipped in alcohol with a volume concentration of 70% for 10-20s , shake the mangosteen seeds while soaking, rinse 2-4 times with sterile water; finally soak in the disinfectant solution with a volume concentration of 7% sodium hypochlorite and 0.2% Tween 80 for 15-20min, shake the mangosteen seeds while soaking, and rinse with sterile water Rinse 3-5 times, and the mangosteen seeds after surface disinfection and sterilization are ready for use; in order to avoid the reduction of the activity of mangosteen seeds, the seeds taken out of the fruit must be processed within 5 days;

[0039] (2) Induction culture of adventit...

Embodiment 3

[0046] The mangosteen seed that obtains from ripe mangosteen fruit, comprises the following steps:

[0047] (1) Surface sterilization of explants

[0048] Mangosteen seeds obtained from mature mangosteen fruits, washed 1-2 times with an aqueous solution containing 3% washing powder or detergent or rinsed with running water for 1-5 hours; then dipped in alcohol with a volume fraction of 75% for 20-30s , shake the mangosteen seeds while dipping, rinse 2-4 times with sterile water; finally soak in the disinfectant solution with a volume concentration of 5-8% sodium hypochlorite and 0.3% Tween 80 for 15-20min, shake the mangosteen seeds while soaking, without Rinse with bacterial water for 3-5 times, and the mangosteen seeds after surface disinfection and sterilization are ready for use; in order to avoid the reduction of the activity of mangosteen seeds, the seeds taken from the fruit must be processed within 5 days;

[0049] (2) Induction culture of adventitious embryos

[005...

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Abstract

The invention relates to a plant tissue culture technique, and discloses a rapid propagation and cultivation method of mangosteen. The method comprises the following steps: (1) sterilization of an explant surface; (2) induction stage of adventitious embryos; (3) explant multiplication stage; and (4) root induction of regeneration buds. Mangosteen seedlings are bred by applying the rapid propagation cultivation method of the mangosteen disclosed by the invention, the multiplication coefficient can reach about 5.4, the obtained test-tube plantlet preserves excellent characteristics of a maternal plant, and does not generate a character separation phenomenon, and the seedlings are good in growth condition. About 1500 mangosteen seedlings can be generated from one seed by cultivation one year, so that the target of quickly and greatly breeding the mangosteen seedlings is achieved, and the method has great practical significance on the mangosteen planting industry.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for rapid propagation of mangosteen. Background technique [0002] The traditional breeding of mangosteen (Garcinia Mangostana L.) can be propagated by seeds, but the vitality of mangosteen seeds cannot be maintained for a long time, and the seed reproduction coefficient of mangosteen is low, and the number of seeds is small. One fruit can produce at most 2 seeds (many fruits have no seeds) , These factors have seriously limited the rapid and large-scale breeding of mangosteen. In addition, due to the characteristics of the mangosteen tree itself, seeds cannot be produced every year, resulting in insufficient supply of seeds; secondly, it takes 10-15 years for seedlings to bloom and bear fruit. The growth cycle is long and the cost of industrial development is high, making it difficult to meet market demand. In the process of mangosteen propagation, there a...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 李敬阳金志强明建鸿唐粉玲林妃许奕常胜合
Owner HAIKOU EXPERIMENTAL STATION CHINESE ACAD OF TROPICAL AGRI SCI
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