Novel phenolic compound treating hepatitis b, and preparation method and application thereof
A compound and a new type of technology, applied in the field of medicine, to achieve a good dose-dependent relationship, inhibit HBsAg and HBeAg, and reduce hepatic steatosis
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Embodiment 1
[0079] Take 50 kg of Toosendan, heat and reflux with 400L deionized water to extract 3 times, the extraction time is 2, 2, and 1 hour respectively, the water extracts are combined, filtered, concentrated under reduced pressure at 60°C to a relative density of 1.05, and poured into the concentrated solution Add ethanol until the ethanol concentration is 80%, precipitate overnight, filter, combine the filtrates, recover the solvent under reduced pressure to a concentration of about 2.0g crude drug / ml, filter, put the filtrate on a macroporous adsorption resin column HZ816, and the resin consumption is 50kg, Control the flow rate to 0.2-0.5 times the column volume / h, successively use 200L deionized water, 150L 10% ethanol to remove impurities, and then use 150L 70% ethanol to elute, collect the alcohol eluate, concentrate under reduced pressure, vacuum dry, and pulverize, Obtain neem extract 240g, neem phenolic compound content is 20.3%.
Embodiment 2
[0081] Take 150 kg of neem leaves, heat and reflux with 900L 80% ethanol to extract twice, the extraction time is 3 and 2 hours respectively, the ethanol extracts are combined, filtered, concentrated under reduced pressure at 60°C, and the solvent is recovered to a concentration of about 2.0g of crude drug / ml, filtered, and the filtrate was put on a macroporous adsorption resin column HZ801, the amount of resin was 120kg, the flow rate was controlled at 0.2-0.5 times the column volume / h, eluted with 300L deionized water, 250L10% ethanol to remove impurities, and then 250L60% Eluted with ethanol, collected the ethanol eluate, concentrated under reduced pressure, dried in vacuum, and pulverized to obtain 906 g of neem extract, the content of neem phenolic compounds was 24.5%.
Embodiment 3
[0083] Take 50kg of neem bark, extract 3 times with 400L60% acetone, the extraction time is 48, 48, and 24 hours respectively, combine the acetone extracts, filter, recover the solvent under reduced pressure to a concentration of about 2.0g crude drug / ml, filter, The filtrate is put on the macroporous adsorption resin column XAD-2, the amount of resin is 50kg, the flow rate is controlled at 0.2-0.5 times the column volume / h, and the impurity is first eluted with 200L deionized water, and then eluted with 100L20% ethanol. Eluted with 150L of 60% ethanol, collected 60% ethanol eluate, concentrated under reduced pressure, dried in vacuum, and pulverized to obtain 290 g of neem extract, the content of neem phenolic compounds was 25.4%.
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