Novel phenolic compound treating hepatitis b, and preparation method and application thereof

A compound and a new type of technology, applied in the field of medicine, to achieve a good dose-dependent relationship, inhibit HBsAg and HBeAg, and reduce hepatic steatosis

Inactive Publication Date: 2014-12-24
TONGLING GINGER SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report that the active ingredients in neem have the effect of treating hepatitis B

Method used

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  • Novel phenolic compound treating hepatitis b, and preparation method and application thereof
  • Novel phenolic compound treating hepatitis b, and preparation method and application thereof
  • Novel phenolic compound treating hepatitis b, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Take 50 kg of Toosendan, heat and reflux with 400L deionized water to extract 3 times, the extraction time is 2, 2, and 1 hour respectively, the water extracts are combined, filtered, concentrated under reduced pressure at 60°C to a relative density of 1.05, and poured into the concentrated solution Add ethanol until the ethanol concentration is 80%, precipitate overnight, filter, combine the filtrates, recover the solvent under reduced pressure to a concentration of about 2.0g crude drug / ml, filter, put the filtrate on a macroporous adsorption resin column HZ816, and the resin consumption is 50kg, Control the flow rate to 0.2-0.5 times the column volume / h, successively use 200L deionized water, 150L 10% ethanol to remove impurities, and then use 150L 70% ethanol to elute, collect the alcohol eluate, concentrate under reduced pressure, vacuum dry, and pulverize, Obtain neem extract 240g, neem phenolic compound content is 20.3%.

Embodiment 2

[0081] Take 150 kg of neem leaves, heat and reflux with 900L 80% ethanol to extract twice, the extraction time is 3 and 2 hours respectively, the ethanol extracts are combined, filtered, concentrated under reduced pressure at 60°C, and the solvent is recovered to a concentration of about 2.0g of crude drug / ml, filtered, and the filtrate was put on a macroporous adsorption resin column HZ801, the amount of resin was 120kg, the flow rate was controlled at 0.2-0.5 times the column volume / h, eluted with 300L deionized water, 250L10% ethanol to remove impurities, and then 250L60% Eluted with ethanol, collected the ethanol eluate, concentrated under reduced pressure, dried in vacuum, and pulverized to obtain 906 g of neem extract, the content of neem phenolic compounds was 24.5%.

Embodiment 3

[0083] Take 50kg of neem bark, extract 3 times with 400L60% acetone, the extraction time is 48, 48, and 24 hours respectively, combine the acetone extracts, filter, recover the solvent under reduced pressure to a concentration of about 2.0g crude drug / ml, filter, The filtrate is put on the macroporous adsorption resin column XAD-2, the amount of resin is 50kg, the flow rate is controlled at 0.2-0.5 times the column volume / h, and the impurity is first eluted with 200L deionized water, and then eluted with 100L20% ethanol. Eluted with 150L of 60% ethanol, collected 60% ethanol eluate, concentrated under reduced pressure, dried in vacuum, and pulverized to obtain 290 g of neem extract, the content of neem phenolic compounds was 25.4%.

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PUM

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Abstract

The invention discloses a novel phenolic compound treating hepatitis b, and a preparation method and application thereof. The preparation method of the novel melia phenolic compound comprises: using a hot extraction method or a cold extraction method to process a melia azedarach raw material, so as to obtain a melia azedarach crude extractive; and then using a methyl propene synthesis adsorbent or an aromatic (styrene, divinyl benzene) serial synthesis adsorbent, a gel synthesis adsorbent and a reverse-phase silica gel adsorbent to perform purification, so as to obtain the novel melia phenolic compound. The invention also discloses application of the novel melia phenolic compound to prepare medicines for treating hepatitis b.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a novel polyphenol compound for treating hepatitis B, its preparation method and application. Background technique [0002] Viral diseases seriously endanger people's life and health and are the main infectious diseases of human beings. The spread is extremely wide, the incidence rate is high, and there are many kinds of viruses, which multiply in cells and can infect different tissue cells to cause diseases. According to incomplete statistics, among human infectious diseases, viral diseases account for as high as 60-65%. According to the type of onset, it can be divided into: 1) acute epidemic diseases, such as influenza, acute hepatitis caused by hepatitis A or E virus, viral encephalitis caused by Japanese encephalitis virus, etc.; 2) chronic infectious diseases, such as AIDS, B, Hepatitis C patients who carry the virus for a long time can cause liver cirrhosis and liver ca...

Claims

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Application Information

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IPC IPC(8): C07D493/14A61K31/352A61P31/20
CPCC07D493/14
Inventor 昌军
Owner TONGLING GINGER SCI
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