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Toad skin composition, preparation method and application thereof

A composition, the technology of toad skin, applied in the field of toad skin composition, its preparation, and toad skin extract, can solve the problems of toxic and side effects, and achieve the effects of small toxic and side effects, high clinical safety, and good analgesic effect

Inactive Publication Date: 2014-11-26
INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Such ingredients have obvious cytotoxic anti-tumor activity, but also have strong toxic and side effects

Method used

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  • Toad skin composition, preparation method and application thereof
  • Toad skin composition, preparation method and application thereof
  • Toad skin composition, preparation method and application thereof

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Experimental program
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Embodiment

[0029] 1. Preparation-1 of cinobufacin polypeptide composition:

[0030] (1) take the dried toad skin and boil it in water to obtain the intermediate extract;

[0031] The specific operation method is: take dry toad skin for repairing, add 8 times of water (V / m), decoct twice for 30 minutes each time, filter, combine the filtrates, and concentrate. Concentrate to a small volume (relative density is 1.01-1.35, 80°C), cool to 40°C, add 95% ethanol to adjust the alcohol concentration to 40-60%, carry out alcohol precipitation once, then pour out the supernatant; recover the supernatant Ethanol until there is no alcohol smell, add 95% ethanol to adjust the alcohol concentration to 75-90%, carry out secondary alcohol precipitation, after standing still, filter under normal pressure, concentrate the filtrate to a thick paste, refrigerate for 24 hours, and centrifuge to obtain the intermediate.

[0032] (2) Sephadex G10, G15, G25, G50, G75 or LH20 is used to separate and purify the ...

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Abstract

The invention discloses a toad skin extract, a preparation method and application thereof. Especially, the toad skin extract is extracted from toad skin, Sephadex and other macromolecular fillers are employed for separation and purification to obtain a polypeptide effective part with a molecular weight of 1000-10000 (Da). The purity of the polypeptide prepared by the method can be controlled above 70%. Pharmacological experiment results show that the composition has an anti-tumor effect and an analgesic effect, and can be used for development of auxiliary anticancer drugs or medical products treating or alleviating pains.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine, and in particular relates to a toad skin composition, its preparation method and application. It particularly relates to a toad skin extract mainly composed of polypeptides, and its molecular weight range is 1000-10000 (Da). Background technique [0002] Toad skin is the dry skin of Bufo Bufo Bufo or Bufo Black Orbitus, which has the effects of clearing away heat and detoxification, diuresis and reducing swelling. A number of drugs developed from the water-soluble parts of toad skin have been launched, especially cinobufacin injection, which is a water-soluble preparation processed from the skin of Bufo bufo. The combined effective rate is 56%, the tumor shrinkage rates are 10% and 16%, and it has synergistic effect on chemotherapy and radiotherapy. Clinical application shows that cinobufacin injection has the effects of detoxification, detumescence and pain relief. It is u...

Claims

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Application Information

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IPC IPC(8): A61K35/56A61K38/02A61P35/00A61P25/04A61P29/00
Inventor 边宝林司南杨健王宏洁赵海誉
Owner INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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