A preparation method of silicon dioxide/octacalcium phosphate particles releasing methylene blue dimer

Abstract

The invention provides a method for preparing silicon dioxide / octacalcium phosphate granules for releasing methylene blue dimer, belonging to the technical field of chemical raw material production. The method comprises the following steps: sequentially adding ammonia water, methylene blue and tetraethoxysilane into a solution with water and ethanol, stirring for 4 hours, centrifuging, washing with ethanol, and preparing a silicon dioxide colloidal solution containing methylene blue by using ethanol; further adding citric acid, ethylenediamine tetraacetic acid disodium, calcium nitrate and diammonium hydrogen phosphate into the solution with water and ethanol, adjusting the pH value of the solution to be 9, further adding polyethylene glycol, stirring for one hour, pouring the silicon dioxide colloidal solution containing methylene blue, stirring for 3 hours, centrifuging, washing, and drying, thereby obtaining the silicon dioxide / octacalcium phosphate granules for releasing methylene blue dimer. The method is simple in process, low in raw material price and easy for industrialization production. The granules prepared by using the method are good in biocompatibility and easy to degrade. The methylene blue is released into a solution from interior of the granules mainly in a dimer molecule manner and is slow to release.

Description

technical field [0001] The invention discloses a method for preparing silicon dioxide / octacalcium phosphate particles releasing methylene blue dimer, belonging to the field of chemical raw material preparation methods. Background technique [0002] Methylene blue is widely used in different fields. It is mainly used for fluorescent tracing after staining of biological cells and for sterilization by generating singlet oxygen molecules after photosensitive oxidation reactions. It is also used for the treatment of diseases such as malaria and urinary tract infections. [0003] In aqueous solution, methylene blue molecules often exist in the form of monomers, dimers, and polymers, and the aggregation shape of molecules has a significant impact on its fluorescence properties, photosensitive oxidation reactions, and redox properties. According to TardivoJP et al (TardivoJP, GiglioAD, OliveiraCS, GabrielliDS, JunqueiraHC, TadaDB, SeverinoD, TurchielloRF, BaptistaMS, Phtodiagnosis P...

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Problems solved by technology

However, the method of simply relying on increasing the concentration to provide methylene blue dimer in the water body will have adverse effects on the health of the cultured aquatic products, and will also deepen the color of the water body, which will have an adverse effect on the water body environment such as the permeability of the water body.

[0005] If the methylene blue molecules are released from the particles into the solution in the form of dimers, it will effectively improve the adverse effects caused by the increase of methylene blue concentration, but the research on the release of methylene blue dimers from the particles has not been reported yet.

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