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A method for preparing ganoderma triterpenoids

A technology of Ganoderma lucidum triterpenes and compounds, which is applied in the field of preparation of Ganoderma lucidum triterpenes, can solve the problems of expensive medicinal research value, not yet put into industrial production, and large demand for raw materials, so as to achieve automatic batch processing and increase yield , the effect of high sensitivity

Inactive Publication Date: 2017-07-25
山东仙草生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Ganoderma lucidum triterpenoids, as active ingredients in Ganoderma lucidum, have great medicinal research value, but their content is very low, extraction is time-consuming, and the demand for raw materials is large, so it has not yet been put into actual industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Preparation before ultra-high pressure treatment

[0030] The ganoderma lucidum fruiting bodies were dried in a drying oven at 40°C for 8 hours, crushed through a 10-mesh sieve through a traditional Chinese medicine pulverizer. Take 2g of Ganoderma lucidum powder, add water and ethyl acetate, and put it into a plastic bag for vacuum sealing.

[0031] (2) Determination of material-liquid ratio

[0032] According to the ratio of material to liquid 1:10:10, 1:20:10, 1:10:20, Ganoderma lucidum powder, water, and ethyl acetate were mixed in a plastic bag, put into an ultra-high pressure device, and the pressure was adjusted to 300MPa. After treatment at room temperature for 5 minutes, the content of triterpenes in Ganoderma lucidum extract was determined, and the yields were 1.98%, 2.12%, and 1.72%, respectively, and the yields of polysaccharides were 1.79%, 1.97%, and 1.82%. The experimental results showed that when the ratio of solid to liquid was 1:20:10 (raw materi...

Embodiment 2

[0042] (1) Dry the fruiting bodies of Ganoderma lucidum in an oven at 40°C for 8 hours, pulverize them into powder with a traditional Chinese medicine grinder, and pass through a 10-mesh sieve;

[0043] (2) Accurately weigh 2g of Ganoderma lucidum fruiting body powder, add water and ethyl acetate at a material-to-liquid ratio of 1:20:10, put it into a plastic bag, vacuum seal it, and place it in an ultra-high pressure device;

[0044] (3) Set the pressure at 295MPa, the temperature at 45°C, and perform ultra-high pressure treatment for 5 minutes;

[0045](4) After depressurization, take out the plastic bag, filter the sample after ultra-high pressure treatment, and collect the triterpenes in the ethyl acetate layer as samples. Rinse the solid-phase column with methanol, acetonitrile, and water of equal column volume successively to activate it, then mix the sample with water and take one column volume for solid-phase extraction C 18 Column, adjust the pH to 4, control the flo...

Embodiment 3

[0048] (1) Ganoderma lucidum fruiting bodies were dried in an oven at 40°C for 8 hours, pulverized into powder with a traditional Chinese medicine pulverizer and passed through a 10-mesh sieve;

[0049] (2) Accurately weigh 2g of Ganoderma lucidum fruiting body powder, add water and ethyl acetate at a material-to-liquid ratio of 1:18:10, put it into a plastic bag, vacuum seal it, and place it in an ultra-high pressure device;

[0050] (3) Set the pressure at 295MPa, the temperature at 45°C, and perform ultra-high pressure treatment for 5 minutes;

[0051] (4) After depressurization, take out the plastic bag, filter the sample after ultra-high pressure treatment, and collect the triterpenes in the ethyl acetate layer as samples. Rinse the solid-phase column with methanol, acetonitrile, and water of equal column volume successively to activate it, then mix the sample with water and take one column volume for solid-phase extraction C 18 For the column, adjust the pH to 4, contro...

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Abstract

The invention belongs to the technical field of natural product extraction and particularly relates to a method for preparing lucid ganoderma triterpenoids. The lucid ganoderma triterpenoids are prepared by adopting an ultrahigh pressure-solid phase extraction combined technology. The method mainly comprises the following steps: drying and crushing; ultrahigh pressure treatment; filtering and collecting; solid-phase extraction; spin evaporating; and vacuum freezing and drying. Compared with a traditional extraction method, the method disclosed by the invention has the advantages that the time is saved by over 90%, the extraction rate of triterpenoids is increased, the economic benefits are improved, solvents is recycled, the solvent consumption is reduced, the method is environment-friendly and free of pollution; due to adoption of solid-phase extraction, the effect of high sensitivity is achieved, a lot of solvents can be saved, and automated batch treatment can be realized.

Description

[0001] (1) Technical field [0002] The invention belongs to the technical field of natural product extraction, in particular to a method for preparing ganoderma triterpenoids. [0003] (2) Background technology [0004] Ganoderma lucidum is a fungus of the genus Ganoderma lucidum in the family Polyporaceae. It is also known as fairy grass and embroidered grass. Since ancient times, it has been widely used in Asian countries as a valuable traditional Chinese medicine that nourishes, strengthens and strengthens the body. With the success of artificial cultivation of Ganoderma lucidum and the realization of mass production, the edibility of Ganoderma lucidum has been generally accepted. The medicinal value of Ganoderma lucidum was first recognized in the 2000 edition of "Chinese Pharmacopoeia". It has been scientifically proven that Ganoderma lucidum contains a variety of active ingredients, mainly including polysaccharides, triterpenoids, alkaloids, nucleosides, furan derivativ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/074
CPCA61K36/074A61K2236/17A61K2236/333A61K2236/51
Inventor 王成忠袁亚光赵晓红周晓
Owner 山东仙草生物科技有限公司
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