Detection method of penetration efficiency of penetrant for preventing and treating citrus Huanglongbing disease
A citrus greening disease and detection method technology, applied in the direction of color/spectral characteristic measurement, etc., can solve the problems such as the application of penetrants to the skin of leaves of plants susceptible to Huanglongbing, improve the transdermal transport efficiency, improve the control effect, Effects in simple steps
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[0029] 1. Separation of Leaf Epidermis
[0030] Take the lemon leaves susceptible to Huanglongbing, use a hole puncher with a diameter of 7mm to obtain a leaf disk with a diameter of 7mm, and completely immerse the leaf disk in 50mM, pH value of 4.0 containing 2% pectinase and 0.2 % cellulase in citrate buffer. After the epidermis was completely separated from the leaves, the epidermis was rinsed with a citric acid buffer solution with a pH value of 4.0, and allowed to dry.
[0031] 2. Detection of penetration efficiency of penetrant
[0032] (1) the lemon leaf disc epidermis of the huanglongbing susceptibility that is obtained by step 1 is placed on the through hole 3 on the osmotic device dividing plate, this osmotic device is isolated two mutually independent chambers, fixes blade with silica gel sealing At the junction of the epidermis and the container, there are two independent chambers, one as the donor chamber 1 and the other as the acceptor chamber 2;
[0033] (2) ...
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