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Detection method of penetration efficiency of penetrant for preventing and treating citrus Huanglongbing disease

A citrus greening disease and detection method technology, applied in the direction of color/spectral characteristic measurement, etc., can solve the problems such as the application of penetrants to the skin of leaves of plants susceptible to Huanglongbing, improve the transdermal transport efficiency, improve the control effect, Effects in simple steps

Inactive Publication Date: 2015-01-07
GUANGXI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are no relevant literature reports on the application of penetrants for the leaf epidermis of Huanglongbing disease-susceptible plants

Method used

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  • Detection method of penetration efficiency of penetrant for preventing and treating citrus Huanglongbing disease
  • Detection method of penetration efficiency of penetrant for preventing and treating citrus Huanglongbing disease
  • Detection method of penetration efficiency of penetrant for preventing and treating citrus Huanglongbing disease

Examples

Experimental program
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Effect test

Embodiment 1

[0029] 1. Separation of Leaf Epidermis

[0030] Take the lemon leaves susceptible to Huanglongbing, use a hole puncher with a diameter of 7mm to obtain a leaf disk with a diameter of 7mm, and completely immerse the leaf disk in 50mM, pH value of 4.0 containing 2% pectinase and 0.2 % cellulase in citrate buffer. After the epidermis was completely separated from the leaves, the epidermis was rinsed with a citric acid buffer solution with a pH value of 4.0, and allowed to dry.

[0031] 2. Detection of penetration efficiency of penetrant

[0032] (1) the lemon leaf disc epidermis of the huanglongbing susceptibility that is obtained by step 1 is placed on the through hole 3 on the osmotic device dividing plate, this osmotic device is isolated two mutually independent chambers, fixes blade with silica gel sealing At the junction of the epidermis and the container, there are two independent chambers, one as the donor chamber 1 and the other as the acceptor chamber 2;

[0033] (2) ...

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Abstract

The invention discloses a detection method of penetration efficiency of a penetrant for preventing and treating citrus Huanglongbing disease. The detection method comprises the following steps of clamping surfaces of leaves of rutaceous aurantioideae plant suffering from citrus Huanglongbing disease between an acceptor chamber and a donor chamber, carrying out sealing fixing by silica gel, adding a boric acid buffer containing a penetrant to be detected into the acceptor chamber, adding a marker-containing citric acid buffer into the donor chamber, arranging the device in the dark, simultaneously, carrying out penetration by a shaking table, then determining marker content in the acceptor chamber respectively at the moment of 0min, 15min, 30min, 45min and 60min after placement and calculating a penetration parameter P according to a formula of P=F / cA. The detection method utilizes the characteristics of the surfaces of leaves of rutaceous aurantioideae plant suffering from citrus Huanglongbing disease, has simple processes and high reliability, can fast select a high-performance penetrant from many penetrants, is conducive to improvement of transdermal transport efficiency of the drug and improves citrus Huanglongbing disease prevention and treatment effects.

Description

technical field [0001] The invention relates to a method for detecting the penetration efficiency of a penetrant for preventing and treating citrus huanglongbing, belonging to the field of agricultural technology. Background technique [0002] Citrus huanglongbing is a devastating disease of citrus production in the world. It is caused by a Gram-negative bacterium (Ca. A variety of Rutaceae plants. After the citrus is infected with Huanglongbing, it will form uniform yellowing or mottled yellowing, and later show a series of typical symptoms such as zinc deficiency and mosaic leaves, which are characterized by short economic life, low yield, and poor fruit quality, resulting in huge economic losses. [0003] Since it was first reported in South my country at the beginning of the 20th century, it has occurred successively in major citrus producing areas in the world, especially causing major economic losses to the citrus industries in Florida, the United States and Sao Paulo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
Inventor 张木清杨川毓
Owner GUANGXI UNIV
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