Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A rapid method for detection of barnyardgrass resistance

A drug resistance and barnyardgrass technology, which is applied in the field of rapid detection of barnyardgrass resistance to herbicides, can solve the problems of expensive test reagents and expensive detection instruments

Active Publication Date: 2016-02-10
HUNAN PLANT PROTECTION INST
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, some methods use expensive detection instruments, such as chloroplast resistance assay and fluorescent signal detection method
There are also some methods that involve expensive test reagents that require professional technicians to complete, such as the target resistance molecular detection method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Rapid detection method of barnyardgrass resistance to quinclorac

[0024] After breaking the dormancy of the collected barnyardgrass seeds, soak them in 0.25% potassium nitrate at room temperature for 1 day, filter out the excess water, and then place them in the incubator for cultivation. Continue to cultivate in the nutrient solution. When the cultivation reaches the 1-leaf stage, paste 10 barnyardgrass seedlings on a plastic board with a length of 15 cm and a width of 7 cm, and then place the plastic board obliquely in the cup filled with the nutrient solution of Feiwanjia. Continue to grow, keep the plant roots immersed in water during cultivation, and wait for barnyardgrass to be cultivated to 2 leaves before use.

[0025] Screening for screening concentrations:

[0026] Taking the field dose of quinclorac as a reference, first set up 6 treatments of 0, 0.05, 0.5, 5, 50, 500, 5000ga.i. / hm with a 10-fold gradient 2 , each treatment was repeated 3 times, and the pl...

Embodiment 2

[0032] Rapid detection method of barnyardgrass resistance to penoxsulam

[0033] After breaking the dormancy of the collected barnyardgrass seeds, soak them in 0.25% potassium nitrate at room temperature for 1 day, filter out the excess water, and then place them in the incubator for cultivation. Continue to cultivate in the nutrient solution. When the cultivation reaches the 1-leaf stage, paste 10 barnyardgrass seedlings on a plastic board with a length of 15 cm and a width of 7 cm, and then place the plastic board obliquely in the cup filled with the nutrient solution of Feiwanjia. Continue to grow, keep the plant roots immersed in water during cultivation, and wait for barnyardgrass to be cultivated to 2 leaves before use.

[0034] Screening for screening concentrations:

[0035] Taking the field dose of penoxsulam as a reference, first set up 6 treatments with a 10-fold gradient of 0, 0.0025, 0.025, 0.25, 2.5, 25, and 250ga.i. / hm 2 , each treatment was repeated 3 times, an...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for rapidly detecting barnyardgrass drug resistance, comprising the following steps: (1) collecting barnyardgrass seeds: collecting herbicide-sensitive barnyardgrass seeds as materials; (2) cultivating barnyardgrass: combining the steps ( 1) After the collected barnyardgrass seeds break dormancy, soak them in potassium nitrate to accelerate germination, then put the germinated barnyardgrass into the nutrient solution and cultivate until 2 leaves are ready for use; (3) Treatment of barnyardgrass: put the The barnyardgrass cultivated to 2 leaves sticks to the plastic plate, and then soaked in herbicide solutions with different gradient concentrations for 30 minutes, then taken out to dry naturally, put into the nutrient solution and continued to cultivate for 15 days, and evaluated the barnyard grass on the leaves. Changes in color, new leaf growth and root development, investigation of barnyardgrass mortality, and obtaining the screening concentration of herbicides can quickly detect whether barnyardgrass has developed resistance to herbicides. The method of the invention can detect large quantities of barnyardgrass samples at one time, and has the advantages of short time, accuracy and reliability.

Description

technical field [0001] The invention relates to the technical field of plant protection, in particular to a method for quickly detecting whether barnyardgrass is resistant to herbicides. Background technique [0002] barnyardgrass ( Echinochloacrus galli ) is one of the top ten malignant weeds in the world, and it is also one of the most serious weeds on the main crops in our country. The harm to rice production is particularly serious, not only the area of ​​damage is large, but also the most significant reduction in rice production. [0003] At present, chemical control is still the most important way to control barnyardgrass. Because barnyardgrass is widely distributed in farmland around the world, it is very harmful. Therefore, the development of herbicides mostly takes barnyardgrass as the main control target, and various types of barnyard grasses with different mechanisms of action have been developed one after another. [0004] However, barnyardgrass has developed...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/00
Inventor 马国兰李巳夫刘都才刘雪源唐涛彭亚军
Owner HUNAN PLANT PROTECTION INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com