A rapid method for detection of barnyardgrass resistance
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A drug resistance and barnyardgrass technology, which is applied in the field of rapid detection of barnyardgrass resistance to herbicides, can solve the problems of expensive test reagents and expensive detection instruments
Active Publication Date: 2016-02-10
HUNAN PLANT PROTECTION INST
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Problems solved by technology
However, some methods use expensive detection instruments, such as chloroplast resistance assay and fluorescent signal detection method
There are also some methods that involve expensive test reagents that require professional technicians to complete, such as the target resistance molecular detection method
Method used
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Embodiment 1
[0023] Rapid detection method of barnyardgrass resistance to quinclorac
[0024] After breaking the dormancy of the collected barnyardgrass seeds, soak them in 0.25% potassium nitrate at room temperature for 1 day, filter out the excess water, and then place them in the incubator for cultivation. Continue to cultivate in the nutrient solution. When the cultivation reaches the 1-leaf stage, paste 10 barnyardgrass seedlings on a plastic board with a length of 15 cm and a width of 7 cm, and then place the plastic board obliquely in the cup filled with the nutrient solution of Feiwanjia. Continue to grow, keep the plant roots immersed in water during cultivation, and wait for barnyardgrass to be cultivated to 2 leaves before use.
[0025] Screening for screening concentrations:
[0026] Taking the field dose of quinclorac as a reference, first set up 6 treatments of 0, 0.05, 0.5, 5, 50, 500, 5000ga.i. / hm with a 10-fold gradient 2 , each treatment was repeated 3 times, and the pl...
Embodiment 2
[0032] Rapid detection method of barnyardgrass resistance to penoxsulam
[0033] After breaking the dormancy of the collected barnyardgrass seeds, soak them in 0.25% potassium nitrate at room temperature for 1 day, filter out the excess water, and then place them in the incubator for cultivation. Continue to cultivate in the nutrient solution. When the cultivation reaches the 1-leaf stage, paste 10 barnyardgrass seedlings on a plastic board with a length of 15 cm and a width of 7 cm, and then place the plastic board obliquely in the cup filled with the nutrient solution of Feiwanjia. Continue to grow, keep the plant roots immersed in water during cultivation, and wait for barnyardgrass to be cultivated to 2 leaves before use.
[0034] Screening for screening concentrations:
[0035] Taking the field dose of penoxsulam as a reference, first set up 6 treatments with a 10-fold gradient of 0, 0.0025, 0.025, 0.25, 2.5, 25, and 250ga.i. / hm 2 , each treatment was repeated 3 times, an...
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Abstract
The invention discloses a method for rapidly detecting barnyardgrass drug resistance, comprising the following steps: (1) collecting barnyardgrass seeds: collecting herbicide-sensitive barnyardgrass seeds as materials; (2) cultivating barnyardgrass: combining the steps ( 1) After the collected barnyardgrass seeds break dormancy, soak them in potassium nitrate to accelerate germination, then put the germinated barnyardgrass into the nutrient solution and cultivate until 2 leaves are ready for use; (3) Treatment of barnyardgrass: put the The barnyardgrass cultivated to 2 leaves sticks to the plastic plate, and then soaked in herbicide solutions with different gradient concentrations for 30 minutes, then taken out to dry naturally, put into the nutrient solution and continued to cultivate for 15 days, and evaluated the barnyard grass on the leaves. Changes in color, new leaf growth and root development, investigation of barnyardgrass mortality, and obtaining the screening concentration of herbicides can quickly detect whether barnyardgrass has developed resistance to herbicides. The method of the invention can detect large quantities of barnyardgrass samples at one time, and has the advantages of short time, accuracy and reliability.
Description
technical field [0001] The invention relates to the technical field of plant protection, in particular to a method for quickly detecting whether barnyardgrass is resistant to herbicides. Background technique [0002] barnyardgrass ( Echinochloacrus galli ) is one of the top ten malignant weeds in the world, and it is also one of the most serious weeds on the main crops in our country. The harm to rice production is particularly serious, not only the area of damage is large, but also the most significant reduction in rice production. [0003] At present, chemical control is still the most important way to control barnyardgrass. Because barnyardgrass is widely distributed in farmland around the world, it is very harmful. Therefore, the development of herbicides mostly takes barnyardgrass as the main control target, and various types of barnyard grasses with different mechanisms of action have been developed one after another. [0004] However, barnyardgrass has developed...
Claims
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