14 results about "TB bacillus" patented technology

The invention discloses a novel compound preparation for resisting mycobacterium tuberculosis and a preparation method thereof.The compound preparation comprises glutathione active part and pyrazinamide, the weight ratio of glutathione to pyrazinamide is (2-4): 5, the glutathione active part comprises glutathione, an isolation stabilizer, a plasticizer, an anti-sticking agent and a first stabilizer, and the first stabilizer comprises glutathione, an isolation stabilizer, a plasticizer, an anti-sticking agent and a second stabilizer. According to the glutathione active part, firstly, a fluidized powder coating technology is adopted, and the relatively unstable active ingredient glutathione is subjected to powder coating; and preparing the coated components, pyrazinamide, a filler, a second stabilizer, an adhesive, a disintegrating agent and a lubricant into a specific preparation. According to the preparation, the hepatotoxicity during pyrazinamide medication is reduced, the compliance is increased, and meanwhile, the technical problem that a pyrazinamide glutathione preparation is unstable is solved.

The application discloses a mycobacterium tuberculosis tandem DNA vaccine W545 and a preparation method and application thereof. The application constructs a novel mycobacterium tuberculosis DNA vaccine W545 with a multi-antigen immune dominant epitope by connecting epitope genes of Ag85A protein and Ag85B protein antigen and Rv1419, Rv3407 and Rv2628c together in series through genetic engineering technology and connecting the epitope genes to a eukaryotic expression vector pVAX1. The prepared mycobacterium tuberculosis tandem DNA vaccine W545 can significantly enhance specific cellular immune function of mice, mainly stimulates Th1 type immune response, and can make the lesion range of organs and tissues of a mouse tuberculosis model significantly reduced and the lesion alleviated in treatment, and can be used in preparation of drugs or vaccines for preventing or treating tuberculosis.

The invention discloses a micromolecule polypeptide capable of inhibiting intracellular survival of mycobacterium tuberculosis and application of the micromolecule polypeptide, and belongs to the technical field of biology. The amino acid sequence of the micromolecule polypeptide is VLARYASICQ (SEQ ID NO.1), and the micromolecule polypeptide has cell penetrability, can promote glycolysis, and further inhibits the survival of mycobacterium tuberculosis in macrophages and mice. The micromolecule polypeptide can be used for preparing a medicine for resisting mycobacterium tuberculosis infection. The results of the invention can provide new tools and ideas for clinical prevention and treatment of tuberculosis, and especially have broad prospects in the aspects of development, clinical application and the like of anti-tuberculosis drugs. The invention has important application value for obtaining novel antituberculosis drugs.

This invention belongs to the field of traditional Chinese medicine oral decoction preparation technology. Specifically, it relates to a method for preparing a stable and safe decoction of traditional Chinese medicine composition for hepatitis, jaundice virus, cirrhosis, and ascites. The decoction is prepared by decocting the following eleven traditional Chinese medicine components: 30g of raw Lysimachia christinae, 30g of Plantago asiatica, 30g of Poria cocos peel, 30g of Dioscorea opposita, 26g of Areca catechu peel, 16g of Salvia miltiorrhiza, 16g of Bupleurum chinense, 16g of Alisma plantago-aquatica, 16g of Astragalus membranaceus, 13g of Lycopus lucidus, and 10g of processed pangolin scales. When decocting, pour the mixture into a sterilized and cleaned porcelain medicine pot, add cold water and soak for 30 minutes. Bring to a boil over high heat, then simmer over low heat for 20 minutes. Turn off the heat and let it cool slightly before drinking. This is the medicine product. By combining classical traditional methods with modern decoction technology, the decoction of these eleven traditional Chinese medicines yields a traditional Chinese medicine preparation with significant effects and convenient administration. This formula features comprehensive regulation and enhancement. It selects herbs such as Lysimachia christinae, Plantago asiatica, Poria cocos, and Salvia miltiorrhiza, which have antibacterial, drainage, dampness-removing, anti-inflammatory, and blood-stasis-removing components for liver ascites. The combination of eleven Chinese herbs has anti-inflammatory, detoxifying, and dampness-removing effects, which are beneficial for treating liver ascites lesions, removing hepatitis, jaundice, tuberculosis, damp-heat accumulation, inflammation-induced ascites, enhancing immunity, and healing effects.

The invention provides a mycobacterium tuberculosis recombinant fusion protein EMC, and the connection sequence of the mycobacterium tuberculosis recombinant fusion protein EMC is as follows: EAST6-MPT64-CFP10. The fusion protein formed by the EAST6 protein, the MPT64 protein and the CFP10 protein has better antigenicity, it is found that effective series connection can more effectively promote mutual complementation of the EAST6 protein, the MPT64 protein and the CFP10 protein in the antigenicity aspect, the number of antigen epitopes is increased, and then the antigenicity of the fusion protein is remarkably improved; and the use dosage of the fusion protein as a detection antigen for immunological diagnosis and an antigen for vaccine development is effectively reduced. The invention also provides a novel tuberculosis intradermal diagnostic reagent and a preparation method thereof.

The invention relates to the technical field of biological detection and medical diagnosis, and discloses a method capable of rapidly detecting mycobacterium tuberculosis, which comprises the following steps: S1, providing a detection test strip which sequentially comprises a sample pad, a combination pad, a chromatography membrane and a water absorption pad; wherein the combination pad is coated with a labeled mycobacterium tuberculosis specific nucleic acid probe, the chromatography membrane is provided with a detection line and a quality control line, capture molecules are fixed on the detection line and the quality control line, the method integrates a nucleic acid probe chromatography technology and simple sample treatment, expensive instruments are not needed, and a result can be obtained by visual inspection within 15 minutes. The kit remarkably reduces the detection threshold and cost, is simple and convenient to operate and high in sensitivity, can synchronously identify drug resistance, perfectly meets the urgent demand of primary medical scenes on rapid and accurate diagnosis of tuberculosis, and assists in early intervention and epidemic prevention and control.

The invention discloses a mycobacterium tuberculosis gene rapid screening service system oriented to a health physical examination center, and relates to the technical field of health services, and the mycobacterium tuberculosis gene rapid screening service system is characterized in that a sample is subjected to automatic uniform mixing and transfer to complete standardized pretreatment; the mechanical arm accurately loads the preprocessed sample to each independent channel of the micro-fluidic chip; bacterial lysis, nucleic acid adsorption, washing and elution are automatically completed in the sealed chip; the channels of the chip are subjected to thermal cycle amplification in parallel and generate target specific fluorescence signals; the system collects fluorescence intensity data of each channel in real time and automatically completes filtering and normalization processing; the trained deep learning model automatically analyzes a fluorescence curve and classifies results; and the system automatically generates an encrypted report and distributes the encrypted report to a subject and a health management center. By integrating closed parallel processing of the multi-channel micro-fluidic chip and fluorescence curve automatic analysis based on deep learning, automation and intelligentization of the whole process from sample loading to report generation can be realized.

The utility model relates to medical detection equipment technical field discloses a kind of mixing device based on tubercle bacillus drug sensitive detection, including storage bucket, the upper portion of the storage bucket is provided with sealing mechanism, the inside of the storage bucket is provided with storage mechanism, the storage mechanism includes motor, the bottom of the motor is fixedly installed in the inside bottom middle portion of storage bucket, the top of the motor is fixedly connected with the shaft of a steam turbine.The utility model drives the rotation of placing disc by bottom motor shaft, and stable mechanical power output can realize the efficient mixing of rotating sample and diluent, centrifugal force and eddy current effect are generated in rotation to promote liquid layering, accelerate uniform mixing, and motor speed is controllable, avoid traditional method to shake unevenly, shorten mixing time, directly reduce drug sensitive detection period, further applicable to high-throughput experimental environment, can support batch processing sample, also can integrate full-automatic detection system, reduce manual intervention, reach laboratory intelligent trend.

The invention relates to a medical sample collection instrument, and discloses a mycobacterium tuberculosis nucleic acid detection sputum sample collection device which comprises a collection pipe, a filter membrane is arranged in the collection pipe, a sealing mechanism is installed at the top of the collection pipe, an extraction mechanism is slidably connected to the top of the sealing mechanism, and the sealing mechanism comprises an opening and closing assembly. The opening and closing assembly comprises a cam disc and a pin shaft, an arc-shaped groove position is formed in the surface of the cam disc, the pin shaft is slidably connected to the inner wall of the inner arc-shaped groove position, a baffle is fixedly connected to the bottom of the pin shaft, a chassis is slidably connected to the bottom of the baffle, a sliding rail is arranged on the surface of the top of the chassis, and the baffle moves along the sliding rail. By means of the arc-shaped groove position structure on the surface of the cam disc, the traction pin shaft slides synchronously, then the baffle is driven to move front and back along the sliding rail at the bottom of the chassis, operation steps are greatly simplified, loading and taking out of samples can be completed more efficiently, and convenience of clinical operation is remarkably improved.

The invention provides a method for detecting the protein content according to the color of a TB-PPD-producing mycobacterium tuberculosis culture filtrate, which is characterized in that a correlation model between color parameters of a mycobacterium tuberculosis culture and the protein content is established through statistical analysis to calculate the protein content in the culture filtrate. The color parameter of the mycobacterium tuberculosis culture is a red (R) color value or a gray value. According to the method, opening sampling detection is not needed, a non-destructive and on-line rapid evaluation reference tool is provided for quality control in the TB-PPD production process, the sterility risk caused by bottle opening detection is reduced, and the production efficiency is improved.

This invention relates to a class of heterocyclic ethylenediazine hexamolybdate tetrabutylammonium derivatives, their preparation methods, and applications, with the chemical formula: (n-Bu4N)3[Mo6O 18 [=N=NCO-HC]; where HC represents pyridinyl, thiophene, furanyl, or pyrazinyl, and n-Bu represents n-butyl; the preparation method includes the following steps: refluxing an organic ligand, a dehydrating agent, and tetrabutylammonium octamolybdate in an organic solvent, wherein the organic ligand is a heterocyclic hydrazide; after reflux, a heterocyclic ethylenediazene hexamolybdate tetrabutylammonium derivative is obtained by separation. This invention utilizes a heterocyclic hydrazide to covalently modify a hexamolybdate cluster, and the resulting heterocyclic ethylenediazene hexamolybdate tetrabutylammonium derivative exhibits certain inhibitory activity against Mycobacterium tuberculosis.

A method for diagnosing Mycobacterium tuberculosis according to an embodiment comprises the steps of: preparing a first diagnosis model trained for diagnosis of Mycobacterium tuberculosis classified as exhibiting normal growth; preparing a second diagnosis model trained for diagnosis of Mycobacterium tuberculosis classified as being affected by a cocktail containing antibiotics; preparing a third diagnosis model trained for diagnosis of other bacteria similar in shape to the Mycobacterium tuberculosis at or above a predetermined level without being affected by the antibiotics; and deriving a diagnosis result of Mycobacterium tuberculosis for a specimen by using a result of diagnosing the specimen acquired through a camera by at least one of the first diagnosis model, the second diagnosis model, and the third diagnosis model.

The invention belongs to the technical field of biological medicines, and particularly discloses application of indolinone compounds in preparation of anti-mycobacterium tuberculosis medicines. The molecular structural formula of the indolinone compound is shown as a formula (A), and the indolinone compound has the advantages of high inhibitory activity, high selectivity, low toxicity and the like as a PafA inhibitor, can be used for developing novel antituberculosis drugs and overcomes the problem of severe drug resistance of drugs for clinically treating tuberculosis.

The invention relates to a heterocyclic ethylene diazene tetrabutylammonium hexamolybdate derivative as well as a preparation method and application thereof. The chemical formula of the heterocyclic ethylene diazene tetrabutylammonium hexamolybdate derivative is (n-Bu4N) 3 [Mo6O18 (= N = NCO-HC), wherein HC represents pyridyl, thienyl, furyl or pyrazinyl, and n-Bu represents n-butyl; the preparation method comprises the following steps: refluxing an organic ligand, a dehydrating agent and tetrabutylammonium octamolybdate in an organic solvent, wherein the organic ligand is heterocyclic hydrazide; and after the reflux is finished, separating to obtain the heterocyclic ethylene diazene tetrabutylammonium hexamolybdate derivative. Heterocyclic hydrazide is utilized to modify a hexamolybdate cluster through a covalent bond, and the obtained heterocyclic ethylene diazene tetrabutylammonium hexamolybdate derivative has certain inhibitory activity on mycobacterium tuberculosis.