267 results about "TB bacillus" patented technology

The present invention relates to fusion proteins containing at least two Mycobacterium species antigens. In particular, it relates to nucleic acids encoding fusion proteins that include two or more individual M. tuberculosis antigens, which increase serological sensitivity of sera from individuals infected with tuberculosis, and methods for their use in the diagnosis, treatment, and prevention of tuberculosis infection.

The invention discloses a fluoroquinolone acetal isoniazone, of which the chemical structure general formula is disclosed as Formula I shown in the description, wherein R1 is hydrogen atom or methyl group; R2 is hydrogen atom or amino group; R3 is hydrogen atom, methyl group, ethyl group, formacyl group, acetyl group, aroyl group or sulfonyl group; R4 is hydrogen atom or methyl group; and X is oxygen atom or sulfur atom. The fluoroquinolone isoniazone disclosed by the invention implements complementarity between the two anti-tuberculosis medicines fluoroquinolone and the isoniazide, lowers the toxic and side effect of the fluoroquinolone and the isoniazide, reduces the generation probability of drug resistance of Mycobacterium tuberculosis for the double-effect antimicrobial agent, and can be used as an anti-Mycobacterium tuberculosis medicine for brand-new structure development of medicinal active substances.

The invention belongs to the field of biomedicine examination, and particularly relates to a kit and a method for detecting mycobacterium tuberculosis infection and application. The invention discloses a novel mycobacterium tuberculosis detection reagent by screening specific T cell epitope of mycobacterium tuberculosis, wherein the reagent contains polypeptide or analog thereof represented by SEQ ID No.1-10. The method detects cell factors released from T cells by using single or more SEQ ID No.1-10 polypeptides to contact the T cells of mycobacterium tuberculosis infected individuals. The method can effectively detect active tuberculosis or latent tuberculosis infection, and is free from disturbance of Bacilli Calmette Guerin (BCG) inoculation vaccines. The invention also discloses a diagnostic kit and other application based on the polypeptide and the method. Compared with the gamma interferon release experiments in the prior art, the method can obviously improve the detection rate without reducing the specificity and has high clinical application value.

The invention belongs to the biomedical detection field, in particular relates to a reagent and method used for detecting activity tuberculosis and latent tuberculosis infection; based on the genomic principle, the invention discloses a novel detection reagent for mycobacterium tuberculosis, containing protein or polypeptide which is represented by SEQ ID1-2, 4-5, 8-28; the method uses one or a plurality of SEQ ID 1-28 protein or polypeptide to contact T cells of a mycobacterium tuberculosis host, and detects cytokine released from the T cells; the method can detect the tuberculosis and latent infection effectively and is not interfered by BCG vaccine at the same time; the invention also discloses a diagnostic reagent kit and other application based on the protein or polypeptide and the method; compared with the T-SPOT of the prior art, the invention can improve detectable rate obviously under the condition that the specificity is not reduced; the reagent kit has cheap price, and the cost is 1 / 5 to 1 / 10 of that of the T-SPOT reagent, thus being beneficial to being popularized in the developing countries and poor areas.

The present invention relates to the identification of the active domain of Herpoxin, a DNA virus-inhibiting-protein which was isolated from cobra venom in U.S. Pat. No. 5,648,339 and has a molecular weight of 13.5 kDa We have isolated a fragment of Herpoxin which contains the active domain and which we have named Herp. Herp mimics the activity of Herpoxin in inhibiting the replication of DNA viruses. A synthetic version of the active fragment was produced having the amino acid sequence Asn-Leu-Tyr-Gln-Phe-Lys-Asn-Met-Ile-Gln. The synthetic version of Herp consisting of ten amino acids inhibits the replication of DNA viruses such as herpes viruses types 1 and 2, cytomegalovirus and varicella zoster virus as well as Tubercle bacilli.

The invention belongs to the technical field of protein structure prediction and drug molecule virtual screening, specifically to a method for screening a compound with targeted action on an inactive conformation of a protein kinase. The method provided by the invention comprises a prediction method for the conformation of an active chain section of the protein kinase, wherein a corresponding DFG-out inactive conformation is generated from the DFG-in active conformation of the protein kinase; the method also comprises a selection method of a combined conformation after implementing butt joint of a II type inhibitor, and the selection method is used for selecting small molecules in conformation prediction and virtual screening. The method for screening a compound with targeted action on an inactive conformation of a protein kinase is already calculated and verified in the protein kinases of seven types of known inactive conformations, wherein the success rate is close to 96%. The method provided by the invention is already applied to the prediction of inactive conformation of PknB protein kinase of tubercle bacillus and the virtual screening of a possible II type inhibitor of PknB, and the bacteriostasis of two kinds of small molecules are already found according to bacteriostatic experiments.

The invention discloses a tuberculosis gene vaccine based on T cell epitopes, wherein a full-length gene, embedded with four T cell epitope polypeptide genes which come from mycobacterium tuberculosis antigen, of mycobacterium tuberculosis heat shock protein is inserted into a vector. The invention also discloses a method for preparing the vaccine, which comprises the following steps: four T cell epitope genes, namely EAST-6189-228, Ag85A369-405, CFP10162-207 and Ag85B420-459 which come from the mycobacterium tuberculosis antigen are inserted into an HSP65 full-length gene. The invention also discloses application of an ECANS tuberculosis gene vaccine. Through the intramuscular injection of the gene vaccine into an immune mouse, the experiment proves that the vaccine can induce a specific antibody which aims at a plurality of tuberculosis antigens to response, can induce stronger tuberculosis specific killing response, can induce Th1 immune response at the same time, secrete high-level IFN gamma, and is a good vaccine for preventing and treating tuberculosis.

The present invention relates to molecular immunology technology, and aims at screening out antigen epitope molecular simulation peptide capable of exciting human body's protective immunoreaction against tubercle bacillus, researching protective immunoreaction mechanism against tuberculosis, and further developing new type of concatenate polyepitope tuberculosis vaccine. The present invention provides one kind of antigen epitope molecular simulation peptide capable of exciting human body's protective immunoreaction against tubercle bacillus, and the peptide contains the amino acid sequence selected from SEQ ID Nos. 2, 5, 10, 12, 14 and 15. The present invention also provides the screening process and use of the peptide. The present invention may be used in preventing and controlling tuberculosis.

The invention discloses a reagent for detecting tubercle bacillus infection in vitro and a method thereof. The reagent comprises M233 polypeptide represented by SEQ ID No.1; and cytokine released from T cells is detected by contacting the M233 polypeptide or an analog thereof with the T cells of a tubercle bacillus host to determine whether the T cells identify the M233 polypeptide or the analog thereof. The reagent has the advantages of high sensitivity, good specificity, being free from interference of BCG vaccine and non-tuberculosis mycobacteria vaccine, being capable of detecting active pulmonary tuberculosis patients, the patients with dormant infection and healthy persons who contact with mycobacterium nontuberculosis. The reagent and the method are especially applicable to detecting tuberculosis and / or dormant infection thereof for Chinese people.

The invention discloses a method for quickly detecting mycobacterium tuberculosis, which comprises the following steps: 1, infecting phage with the mycobacterium tuberculosis; 2, killing the phage notinfecting outside the mycobacterium tuberculosis; 3, amplifying the infecting phage; and 4, after labeling gold with antiphage antibody, adopting quick immunochromatography to detect whether the phage exists or not so as to judge whether the mycobacterium tuberculosis exists in a sample. In addition, the invention also discloses a corresponding kit, which comprises sample treating fluid, proliferous liquid, a killing agent, mycobacterium smegmatis, mycobacteriophages and a phage colloidal gold method immunity-chromatography test pen. The method and the kit can quickly and accurately detect the mycobacterium tuberculosis so as to meet the requirement of quickly diagnosing pulmonary tuberculosis and timely medicate the patient.

The invention belongs to the technical field of pharmaceutical chemistry and discloses a quinoline, and a preparation method and an application of the quinoline. The quinoline is a chemical compound shown as Formula I as shown in the specification, or an optical isomer, racemate, a diastereoisomer, pharmaceutically acceptable salt or solvate of the chemical compound. The quinoline has a significant killing effect on mycobacterium tuberculosis, is applicable to preparing drugs for treating or preventing diseases or symptoms caused by mycobacterium tuberculosis infection.

The invention relates to a diagnostic kit for tuberculosis and mycobacterium tuberculosis infectors, a preparation method and an application method thereof. By using the linker for encoding 15 amino acid (G4S1) 3, the encoding genes (SEQ.ID.NO.6) of a mycobacterium tuberculosis specific antigen Rv3875 and Rv3874 are connected in series, and then inserted into an E. coli expression vector, and the high-efficiency expression and purification for the fusion protein of Rv3875 and Rv3874 in the E. coli are achieved. The recombinant protein at least comprises 8 T cell epipositions which can be used for cell immunity diagnosis, and a diagnostic kit and diagnostic method for a whole blood IFN-Gamma release analysis method are established by taking the protein as the basis and combining the human IFN-Gamma enzyme-linked immunoassay technology, and can be used for the early, specific diagnosis and screening of tuberculosis and mycobacterium tuberculosis infectors.

The invention relates to anti-tubercule bacillus recombination protein. It belongs to gene recombination technique field. Its manufacturing includes the following steps: combining antigen the 190-198 polypeptides of the Ag85B-Mpt64 with Mtb8.4; cloning into escherichia coli vector to express; further purifying to gain. It is recorded as Ag85B-Mpt64190-198-Mtb8.4(SEQ.ID.NO.1). The protein can be used as anti tubercule bacillus subunit vaccine.

The invention discloses a detection method of mycobacterium tuberculosis pyrazinamide drug resistance, comprising the following steps: A. pyrolyzing a template of mycobacterium tuberculosis used for polymerase chain reaction (PCR); B. designing a PCR primer, and augmenting a full-length pyrazinamide (pncA) gene; C. concentrating the augmented pncA gene segment and quantifying; D. expressing pncA enzyme by adopting a wheat germ acellular expression system; and F. determining the expressed pncA enzymatic conversion pyrazinamide into the activity of pyrazine acid; comparing the pncA enzymatic activities of a sensitive strain and a drug resistance strain of the expressed tubercle bacillus standard; and determining the drug resistance of the mycobacterium tuberculosis. The method is characterized in that the drug resistance caused by pncA gene mutation can be determined by a pair of primers, and simultaneously discloses a primer sequence for augmenting the full-length pncA gene from the tubercle bacillus gene group and being suitable for a wheat germ acellular expression system to express the mycobacterium tuberculosis pyrazinamide. By the detection method, the mycobacterium tuberculosis pyrazinamide drug resistance can be rapidly and sensitively detected in no need of bacterial culture.

The invention discloses a method and a kit for detecting drug resistance of mycobacterium tuberculosis. The invention provides special-purpose primers and special-purpose probes for identification of drug resistance of mycobacterium tuberculosis in a sample needing to be detected, wherein the special-purpose primers comprise multiple primer groups; each one of the primer groups comprises a commonprimer and multiple specific primers for amplification of alleles corresponding to all mutational sites of same-purpose genes; the special-purpose probes comprise multiple types of probes; and each one of the probes specifically binds with target sequences corresponding to the common primer and the specific primers of the primer group. Experiments of the invention prove that the special-purpose primers and the special-purpose probes can be combined by an efficient and sensitive duplex Taqman real-time fluorescent polymerase chain reaction (PCR) technology and a sequence specific primer (SSP) amplification technology so that drug resistance of mycobacterium tuberculosis in a detected sample can be detected in a short time.

The invention discloses rhamnus crenata natural plant mosquito-repellent incense capable of calming mind and nerves and a preparation method of the rhamnus crenata natural plant mosquito-repellent incense. Being prepared from natural traditional Chinese medicine compositions, the rhamnus crenata natural plant mosquito-repellent incense is environmentally friendly and safe, has no toxic and side effects, is high in safety performance, can be used for effectively repelling the mosquitoes and further has certain inhibiting and killing effects on multiple germs and fungi such as staphylococcus, mycobacterium tuberculosis, pseudomonas aeruginosa, gram-negative bacteria and gram-positive bacteria; meanwhile, the rhamnus crenata natural plant mosquito-repellent incense has fragrance and mild properties, can refresh mind, has the effects of relieving pressure and effectively improving sleep without generating harmful effects to human bodies and is suitable for crowds of all ages.

The present invention belongs to the field of screening antituberculotic target, vaccine antigen and detecting target in molecular biological technology, and is especially process of screening protein resisting rifampicin as antituberculotic. The process includes the first culturing drug resistant strain, the subsequent separating protein of drug resistant strain from protein of sensitive strain, comparing protein of drug resistant strain and protein of sensitive strain to determine the drug resistant protein, and final separating and identifying drug resistant protein. The process of the present invention can separate and identify drug resistant protein of Mycobacterium tuberculosis to provide new way for further understanding the drug resisting mechanism of Mycobacterium tuberculosis, fast clinical detection of drug resistant strain, and developing vaccine and medicine.

The invention belongs to the technical field of medical treatment, and in particular relates to a purification method of tubercle bacilipin Arab mannan (LAM) and a tubercle bacillus diagnostic reagent. In the invention, by utilizing the structural differences that the LAM antigen contains three mannose while other impurities (LM, PIM, and the like) only contain one mannose, and the appetency of lectin (such as flat lentils lectin) to three manna branch structures is far higher than two manna and one manna, the LAM antigen purification is carried out by using the lectin to obtain the LAM antigen with the purity of above 95%. The high-purity antigen obtained by purification can form a reagent for LAM antigen detection and can also form a reagent for enriching urine LAM antigen, purifying LAM antibody and detecting the urine LAM antibody.

The invention relates to a nitro imidazole compound, its preparation method and use, in particular to a novel nitro imidazole compound, and its pharmaceutically acceptable salt, preparation method and application in preparing medicines for treating mycobacterium tuberculosis infectious diseases, especially infectious diseases caused by multidrug resistant mycobacterium tuberculosis. The nitro imidazole compound or the pharmaceutically acceptable salt thereof disclosed in the invention has good activity against mycobacterium tuberculosis in vitro, especially has strong activity against multidrug resistant mycobacterium tuberculosis.

The invention discloses a preparation method of agilawood health-care wine, which comprises the following steps: 1) raw material preparation; 2) infusion; 3) finished product preparation, that is, filtering and checking infused liquid, then bottling and sealing to prepare the agilawood health-care wine. The invention also discloses agilawood wine prepared by the method. The method provided by the invention is simple in preparation process, easy to realize, high in production efficiency, and capable of rapidly preparing agilawood health-care wine with good health-care effect. The agilawood wine provided in the invention is simple in raw material components, and reasonable in combination, and gives full and effective play to pharmacological effects of agilawood, which means that fragrance and pharmacological components of agilawood are effectively diffused into the wine; the wine has the efficacy of inhibiting mycobacterium tuberculosis, organizing central nervous system, calming, relieving cough, reducing high blood pressure, and resisting heart ischemia, and thus has the effect of health care.

The invention provides a method for identifying mycobacterium tuberculosis. According to the invention, mycobacterium tuberculosis coding gene Rv1508c sequence is adopted as a gene sequence to be detected; and detection is carried out with primers (and probes) designed with the sequence as a detection target. The method provided by the invention has the advantages of good accuracy and high sensitivity. With the method, a novel scheme is provided for the identification of mycobacterium tuberculosis. The method can be used in biological strain identification, tuberculosis infection identification, and researches in respects such as efficacy and mycobacterium tuberculosis drug resistance in drug development processes.

The invention relates to the biological technology field, and provides a preparation method for an ELISA detection kit of goat corynebacterium pseudotuberculosis antibodies. Complete corynebacterium pseudotuberculosis thalluses are employed as coating antigens, an indirect ELISA detection method is established, whether serum contains corynebacterium pseudotuberculosis antibodies is detected and whether a goat is infected with caseous lymphadenitis is determined. The positive and negative critical value is determined, and the detection kit has high specificity and repetition, can detect pseudotuberculosis antibodies in goat serum rapidly and effectively, and can be used for goat pseudotuberculosis diagnosis and epidemiological investigation.

The related detection method for bacillus tubercle specific secretory antigen comprises: culturing or using bio-engineering method to obtain ESAT-6, MPT53, MPT64, MPT63 and MPT70, preparing opposite antigen, and realizing synchronous detection for five antigens. This invention improves detection sensitivity and can provide effective evaluation for diagnosis.

The present invention provides a nanometer probe system for tubercle bacillus detection, and a preparation method for the nanometer probe, and further provides a tubercle bacillus detection method based on the nanometer probe system and the preparation method. The nanometer probe system comprises an integrated nanometer material probe and immunomagnetic beads, wherein the integrated nanometer material probe, the immunomagnetic beads and tubercle bacillus thallus are combined through specific ligands to form a tertiary complex, a magnetic field effect is adopted to rapidly and accurately separate and concentrate the tubercle bacillus and the integrated nanometer probe, a fluorescence signal on the integrated probe is detected, and the detection signal can be significantly amplified through magnetic enrichment and quantity difference between polypeptide sites and fluorescence quantum dots on the integrated nanometer probe so as to achieve accurate, highly sensitive and specific tubercle bacillus detection.

The present invention discloses a grass carp enteritis treatment drug composition, which contains, by weight, 10-30 parts of humulus scandens, 20-30 parts of humifuse euphorbia herb, 2-10 parts of melia azedarach leaf, 20-25 parts of heartleaf houttuynia herb, 2-10 parts of arborvitae leaf, and 2-7 parts of garlic. The invention further discloses a preparation method for the drug composition. According to the grass carp enteritis treatment drug composition, honeysuckle provides an anti-pathogenic microorganism effect, a certain inhibition effect is provided for a variety of pathogenic bacteria such as staphylococcus aureus, hemolytic streptococcus, escherichia coli, shigella dysenteriae, vibrio cholerae, typhoid bacillus, paratyphoid bacillus and the like, and a certain inhibition effect is further provided for pneumococcus, meningococcus, pseudomonas aeruginosa, and tubercle bacillus. The preparation method is simple and easy to perform, and is suitable for mass production.

The present invention provides antibiotics of urinate glucoside peptide category with a structural formula (I) and the acceptable salt in pharmaceutics. The antibiotics can be prepared through fermentation and cultivation of produced fungus. The compound of the present invention is a group of cell-wall peptidoglycan synthesized inhibitor, has anti-tuberculosis activity and pyocyanic fungus, and can be used for preparing anti-infective drugs.

The invention discloses an adenovirus carrier avian influenza recombined vaccine. The vaccine takes the hemagglutinin antigen gene of highly pathogenic avian influenza H5N1 as a main protective antigen gene which is fused with a braided mycobacterium tuberculosis heat shock protein coding gene. After being transferred into an adenovirus carrier to obtain a recombined adenovirus and immunize an animal, the fusion gene can produce a high titer antibody of the hemagglutinin antigen HA inside the animal and keep a high antibody titer inside the animal for a long period.

The invention discloses a reagent for mycobacterium tuberculosis infection detection, clinical treatment effect tracking and antituberculous vaccine development. The novel mycobacterium tuberculosis derived reagent Rv3006 (LPPZ) prepared by a gamma-interferon gamma release assay and enzyme linked immunosorbent assay comprises antigen or polypeptide and analogues thereof, wherein terminal C to terminal N in the amino acid sequence of the Rv3006 antigen is as shown in SEQ ID NO:1. By utilizing the reagent, tuberculous patients in the active phase and tuberculous latent infectors can be well detected; the specific immune response of Rv3006 is reduced along with treatment development and disease condition remission, which indicates that the reagent can be used for well tracking the clinical treatment situation of a tuberculous patient, thus having a good clinical application prospect. Furthermore, the reagent has a protective effect on a mouse infected by a mycobacterium tuberculosis standard toxic strain H37Rv, thus having a good development prospect of antituberculous vaccines.

The invention belongs to the field of medical tests and provides a kit for detecting the genotype of a mycobacterium tuberculosis clinical isolation strain quickly. The kit comprises a detection probe designed aiming at an IS6110 insertion sequence. In a mycobacterium tuberculosis typing method involved in the invention, deoxyribose nucleic acid (DNA) of a sample strain is extracted, polymerase chain reaction (PCR) amplification is performed by taking the detection probe as a primer, and if the obtained product is consistent with a mycobacterium tuberculosis DNA amplification product, a sample comprises mycobacterium tuberculosis. The method is high in resolution ratio of the mycobacterium tuberculosis clinical isolation strain spreading in China currently, the genotype result of every strain is output in a digitization mode, and the genotype results can be compared among different laboratories conveniently. Moreover, a large number of equipment is not needed in the method, the kit can be operated simply, and the price is relatively low. The kit provides a powerful tool for exploring and researching the prevailing and spreading law of tuberculosis in China.

The invention relates to a substituted 1,3-miscellaneous azole compound, a preparation method thereof, a pharmaceutical composition containing the substituted 1,3-miscellaneous azole compound and a purpose thereof. The invention relates to the compound in a formula I, or its pharmaceutically acceptable salt, a stereisomer or a solvate, wherein R1, R2, R3 and X are defined as a specification; and the invention also relates to the preparation method, the pharmaceutical composition containing the substituted 1,3-miscellaneous azole compound and the purpose thereof. The compound of the present invention is the novel antituberculous compound, is effective to mycobacterium tuberculosis-susceptible strains, also possesses activity on strains with tolerance on traditional first-line antituberculous drugs such as isoniazide and rifampicin, and has good selectivity to mycobacterium tuberculosis.