Mother strain culture medium formula of wood-rotting edible fungi and preparation method of mother strain culture medium

Abstract

The invention discloses a mother strain culture medium formula of wood-rotting edible fungi and a preparation method of a mother strain culture medium. The formula comprises the following raw materials in parts by weight: 60-100 parts of peach twigs, 40-60 parts of wheat grains, 40-60 parts of soybean curd, 15-25 parts of multi-vitamin glucose powder, 15-25 parts of agar and 1000 parts of water. The invention further provides the preparation method of the mother strain culture medium and the preparation method comprises the following steps: selecting the raw materials, preparing, subpackaging, sealing, tying up, sterilizing and placing on a slant. After the mother strain culture medium is used for many years, regardless of purification, rejuvenation or subculture, the growth density of mycelia, uniformity and growth speed of hyphae of the wood-rotting edible fungi which are transferred to and cultured in the mother strain culture medium are better than those of edible fungi cultured in a traditional culture medium PDA (potato dextrose agar); and particularly, under the situation of using a silica gel plug, for a mother strain which is preserved at a low temperature in a refrigerator and then transferred after being preserved for 1-2 years, the survival rate can still be more than 95%, and the occurrence of phenomena of variety degeneration, variation and the like can be avoided.

Description

technical field [0001] The patent of the present invention relates to the field of cultivation of edible fungus mother species, in particular to a formula of a wood-rot edible fungus mother species culture medium and a preparation method thereof. Background technique [0002] At present, the nutrition of edible fungi has long been accepted by people, and has become a daily dish on every table. The market demand is increasing, so the planting area is also increasing. Edible fungi can be divided into grass-rot fungi and wood-decay fungi according to the raw materials required for their cultivation. The main raw materials for grass-rot fungi are crop straw and Gramineae straw. The main raw materials are wood chips of broad-leaved trees and cottonseed hulls. The compost of cultivated varieties with crop stalks as the main raw material and varieties cultivated with cottonseed hulls must be composted and fermented. Wood-rot edible fungi refer to edible fungi that are born in the...

AI Technical Summary

Problems solved by technology

Practice has proved that if this type of medium is used for a long time in production, some edible fungus species are prone to weak mycelium growth, reduced stress resistance or species degradation, which has a serious impact on the production and preservation of edible fungi.