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Application of triptolide and tripterine in preparation of antitumor drugs

A technology of triptolide and triptolide, which is applied in the field of preparation of anti-tumor drugs, can solve the problems of no combination of triptolide and triptolide, and achieve a significant superposition of curative effects, broad application prospects, and improved curative effect Effect

Active Publication Date: 2015-03-11
JINAN UNIVERSITY +4
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no relevant literature report on the combined use of triptolide and triptolide

Method used

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  • Application of triptolide and tripterine in preparation of antitumor drugs
  • Application of triptolide and tripterine in preparation of antitumor drugs
  • Application of triptolide and tripterine in preparation of antitumor drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Detection of sensitivity of different tumor cells to triptolide or triptolide

[0028] The sensitivity of different tumor cells to triptolide or triptolide was detected by MTT assay. Human lung cancer cells H1299, H157, H460, H1650 and H1944, ovarian cancer cells OVCAR3 and SKOV3, cervical cancer cells SIHA and HELA, gastric cancer cells BGC823, liver cancer cells BEL7402 and colon cancer cells SW480 were used at a rate of 3000-5000 cells per well. Inoculated into 96-well plate, after the cells adhered to the wall, different concentrations of triptolide or triptolide in PBS solution were added. After culturing for 68 h, add 10 μL of MTT with a concentration of 5 mg / ml to each well and culture for another 4 h, then discard the culture solution and add 100 μL of DMSO to each well, and read the absorbance value of each well at 490 nm with a microplate reader. The half inhibitory concentration (IC) was calculated by the Bliss method 50 ). The specific results ...

Embodiment 2

[0030] Example 2: Detecting the influence of triptolide or triptolide alone and in combination on the survival rate of different tumor cells

[0031] Human lung cancer cells H1299, H157, H460, H1650 and H1944, ovarian cancer cells OVCAR3 and SKOV3, cervical cancer cells SIHA and HELA, gastric cancer cells BGC823, liver cancer cells BEL7402 and colon cancer cells SW480 were used at a rate of 3000-5000 cells per well. Inoculated into 96-well plate, after the cells adhered to the wall, PBS solutions with different ratios of triptolide and triptolide were added. After culturing for 68 h, add 10 μL of MTT with a concentration of 5 mg / ml to each well and culture for another 4 h, then discard the culture solution and add 100 μL of DMSO to each well, and read the absorbance value of each well at 490 nm with a microplate reader. CompuSyn software was used for synergy comprehensive factor (Combination index, CI) analysis. see results figure 2 , image 3 and Table 1.

[0032] The CI...

Embodiment 3

[0035] Example 3: Effects of triptolide and tripterine alone or in combination on the cell cycle of lung cancer cells H1299 and H157.

[0036] The cell cycle arrest of lung cancer cells H1299 and H157 induced by triptolide and tripterine alone or in combination was detected by flow cytometry. First, H1299 and H157 cells were divided into 1 × 10 per well5 Cells were inoculated on a 6-well plate at a density of 100, and after the cells adhered to the wall, the H1299 and H157 cells were divided into four experimental groups, and the following solutions were added respectively: the first group was the control group without treatment; the second group was Leigong The triptolide treatment group alone (wherein the concentration of triptolide for H1299 cells is 0.03μM, the concentration of triptolide for H157 cells is 0.6μM); the third group is triptolide alone treatment group (wherein the concentration of triptolide for H1299 The concentration of triptolide in cells is 0.015 μM, and ...

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Abstract

The invention belongs to the technical field of antitumor drug preparation, and discloses application of triptolide and tripterine combined medication in preparation of antitumor drugs. Through triptolide and tripterine combined medication, triptolide and tripterine have obvious synergistic effect in the dosage range of the invention, the curative effect is effectively improved, compared with a single component or simple curative effect superposition of two components, the curative effect is more remarkable, the destruction of tumor cells is improved; the dosage of each other can be effectively reduced, the toxic and side effect can be reduced, through triptolide and tripterine combined medication, the cost is saved, the economic burden of patients is reduced, and the a new way for prevention and treatment of cancer is provided, and the application prospect in the field of medicines is broad.

Description

technical field [0001] The invention belongs to the technical field of preparation of antitumor drugs, and in particular relates to the application of triptolide and tripterine combined medicine in the preparation of antitumor drugs. Background technique [0002] Malignant tumors are common and frequently-occurring diseases that seriously threaten human health in the world. At present, the main methods for treating tumors are surgery, chemotherapy and radiotherapy. Chemotherapy, as one of the three traditional means of tumor treatment, occupies an important position in tumor treatment. However, most of the chemotherapeutic drugs currently used clinically are not selective in killing tumor cells, have severe side effects, and most of them have immunosuppressive effects. Therefore, the research and development of new anti-tumor drugs with high efficiency and low toxicity from natural medicines has always been a hot spot in the research of anti-tumor drugs. Combined drug ther...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/585A61K31/56A61P35/00
CPCA61K31/56A61K31/585A61K2300/00
Inventor 石智蒋起韦程科军魏星吕敏张旭
Owner JINAN UNIVERSITY
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