A cell physiological information detection system and detection method thereof

Abstract

The invention relates to a cell physiological information detection system and detection method based on planar patch clamp technique and robotization probe control technology. The cell physiological information detection system includes a planar patch clamp module and a nano manipulation robot module which are in connection; the planar patch clamp module is used for cell adsorption, sealing and membrane breaking; and the nano manipulation robot module is used to achieve nano observation and nano manipulation of cells. The cell physiological information detection method is as follows: a macro micro motion platform and a Z-direction nano scanner are controlled to drive a probe to achieve nanometer level displacement; a laser deflection signal is detected by a photoelectric sensor, and then fed back to a data processing and human-computer interaction interface to obtain probe contact force, and ion current synchronous detection can be achieved by an electrode and a signal amplifier. The cell physiological information detection system uses a scanning probe to apply pico-Newton to nano-Newton level supermicro mechanical force stimulation to the cell surface, and at the same time, uses the planar patch clamp to realize detection of currents of mechanically gated ion channels of cells.

Description

Technical field [0001] The invention relates to a multi-parameter living cell physiological information detection technology, in particular to a multi-parameter living cell physiological information detection system and a detection method thereof, which are mainly used in the field of life sciences. technical background [0002] At present, multidisciplinary research based on the cellular level is becoming a new driving force for technological development. The use of micro-observation and micro-manipulation theoretical methods under the physiological state of living cells to extract biomarker information that reflects the state of the body and the reflected cell level will undoubtedly become a key technology for the development of new technologies in the field of life sciences. [0003] The current scientific research based on the cellular level mainly relies on the following two aspects: [0004] Patch clamp technology based on the principle of bioelectric current detection. The u...

AI Technical Summary

Problems solved by technology

At present, the biological atomic force microscope on the market is an effective means for micro-nano observation and manipulation of cells. Unfortunately, such products currently lack the ability to detect cell ion channel currents.

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