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Cultivation and mass-production method of adipose-derived stem cells and stem cell secretion of adipose-derived stem cells

A technology of fat stem cells and fat cells, which is applied in the field of cultivation and mass production of stem cells and their stem cell secretions, can solve the problems of losing the characteristics of stem cells and failing to meet clinical expectations, so as to save complicated operation steps and manpower, and save economic costs , Reduce the effect of equipment space

Inactive Publication Date: 2015-03-18
ISIS BIOMED CO LTD +1
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  • Abstract
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Problems solved by technology

[0003] However, the rapid differentiation ability of stem cells often causes them to gradually lose their stem cell characteristics after the third to fourth passage, so that subsequent experiments often fail to meet clinical expectations

Method used

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  • Cultivation and mass-production method of adipose-derived stem cells and stem cell secretion of adipose-derived stem cells
  • Cultivation and mass-production method of adipose-derived stem cells and stem cell secretion of adipose-derived stem cells
  • Cultivation and mass-production method of adipose-derived stem cells and stem cell secretion of adipose-derived stem cells

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Embodiment Construction

[0019] refer to figure 1 , which is a flow chart showing the steps of the method for culturing adipose stem cells and mass producing their stem cell secretions of the present invention. The present invention mainly provides a method for culturing and mass producing adipose stem cells and stem cell secretions thereof, the steps comprising: collecting adipose cell samples (step 101); isolating adipose stem cells in the adipose cell samples (step 102); The stem cells are distributed on the three-dimensional carrier by the stem cell planting method, and placed in a bioreactor for culturing (step 103); the stem cell secretion secreted by the adipose stem cells in the culture medium is collected (step 104). Wherein the stem cell secretion at least includes vascular endothelial growth factor (Vascular endothelial growth factor, VEGF), hepatocyte growth factor (Hepatocyte growth factor, HGF), interleukin-6 (Interleukin-6, IL-6), type 1 collagen One or a combination of proteins (colla...

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Abstract

The invention discloses a cultivation and mass-production method of adipose-derived stem cells and stem cell secretion of the adipose-derived stem cells. The method comprises the following steps: (A) collecting an adipose cell sample; (B) separating adipose-derived stem cells from the adipose cell sample; (C) planting the adipose-derived stem cells into a three-dimensional carrier, and cultivating in a bioreactor; and (D) collecting stem cell secretion which is secreted by the adipose-derived stem cells, wherein the stem cell secretion at least comprises one of a vascular endothelial growth factor, a hepatocyte growth factor, an interleukin-6 and type 1 collagen, or combination thereof, and therefore, heavy and complicated steps, and economic cost such as manpower and equipment can be removed, adipose-derived stem cells can be quickly and massively cultivated, the stem cell secretion of the adipose-derived stem cells can be produced, and the cultivation and mass-production method can be applied to the fields of disease treatment, cytothesis and regenerative medicine in the future.

Description

technical field [0001] The invention relates to a method for culturing and mass-producing stem cells and stem cell secretions thereof, in particular to a method for cultivating fat stem cells and mass-producing the stem cell secretions thereof. Background technique [0002] Based on the vigorous development of biotechnology and medical technology in recent years, stem cells have become increasingly important in the biomedical industry due to their unique potential for differentiation, proliferation and tissue repair. Whether it is from the number of global stem cell-related companies, the substantial growth in market value, or the year-on-year increase in clinical cases of stem cell applications accepted by the US Food and Drug Administration (FDA), it shows that stem cell therapy has become an international biotechnology industry. key development projects. [0003] However, the rapid differentiation ability of stem cells often causes them to gradually lose their stem cell ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775C12P21/00
Inventor 蔡嘉榉
Owner ISIS BIOMED CO LTD
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