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Method for preparing enhanced DC-CIK cell induced by traditional Chinese medicines and application of enhanced DC-CIK cells induced by traditional Chinese medicines

An enhanced cell technology, applied in the field of biomedicine, can solve the problems of cell specificity, limited activity, high cost and wide application, and short cell retention time, so as to protect bone marrow, improve tumor killing activity and secrete cytokines, The effect of saving cultivation time

Inactive Publication Date: 2015-05-06
杭州特马赛生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after routinely taking peripheral blood from patients and culturing and expanding DC-CIK in vitro, the cells remain in the body for a short time, and the specificity and activity of the cells are limited. Patients need to receive long-term treatment. -Wide application of CIK in clinic

Method used

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  • Method for preparing enhanced DC-CIK cell induced by traditional Chinese medicines and application of enhanced DC-CIK cells induced by traditional Chinese medicines
  • Method for preparing enhanced DC-CIK cell induced by traditional Chinese medicines and application of enhanced DC-CIK cells induced by traditional Chinese medicines
  • Method for preparing enhanced DC-CIK cell induced by traditional Chinese medicines and application of enhanced DC-CIK cells induced by traditional Chinese medicines

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1 (test group)

[0033] 1) Peripheral blood collection

[0034]Use a 50mL syringe to collect 50mL of peripheral blood from the patient, then transfer it equally to two 50mL centrifuge tubes filled with 5mL of heparin (15±2.5IU / mL), tighten the cap of the tube and mix the peripheral blood and heparin thoroughly to prevent blood agglutination .

[0035] 2) Mononuclear cell isolation

[0036] Centrifuge the above two centrifuge tubes containing blood samples at 800g×10min. After centrifugation, discard the upper layer of plasma, then add normal saline to 30ml, mix well, and then add each to the centrifuge tube containing 15ml of lymphocyte separation solution. , centrifuge at 700g×20min to separate mononuclear cells, transfer the mononuclear cells in 2 tubes to a new sterile 50mL centrifuge tube, add normal saline to 50ml, centrifuge at 800g×10min, discard the supernatant, and wash with 0.9 Set the volume to 50ml with % normal saline, and take 300ul of cell s...

Embodiment 2

[0048] Embodiment 2 (control group)

[0049] 1) Peripheral blood collection

[0050] Use a 50mL syringe to collect 50mL of peripheral blood from the patient, then transfer it equally to two 50mL centrifuge tubes filled with 5mL of heparin (15±2.5IU / mL), tighten the cap of the tube and mix the peripheral blood and heparin thoroughly to prevent blood agglutination .

[0051] 2) Mononuclear cell isolation

[0052] Centrifuge the above two centrifuge tubes containing blood samples at 800g×10min. After centrifugation, discard the upper layer of plasma, then add normal saline to 30ml, mix well, and then add each to the centrifuge tube containing 15ml of lymphocyte separation solution. , centrifuge at 700g×20min to separate mononuclear cells, transfer the mononuclear cells in 2 tubes to a new sterile 50mL centrifuge tube, add normal saline to 50ml, centrifuge at 800g×10min, discard the supernatant, and wash with 0.9 Set the volume to 50ml with % normal saline, and take 300ul of ce...

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Abstract

The invention provides a method for preparing an enhanced DC-CIK cell induced by traditional Chinese medicines. The method comprises the step of adding extracts of radix astragali and radix codonopsitis into a DC or CIK cell culture fluid or DC-CIK cell culture fluid or DC, CIK and DC-CIK cell culture fluids, wherein the extracts of radix astragali and radix codonopsitis are radix astragali polysaccharide and radix codonopsitis polysaccharide. According to the method disclosed by the invention, the activity and the functions of the DC or CIK cell can be activated, and the specificity of the DC-CIK cell is improved so that the DC-CIK cell can resist tumor in the body for long time; a large amount of DC-CIK cells can be obtained according to the method, and the cell culture time is reduced; the enhanced DC-CIK cells obtained according to the method disclosed by the invention can be used for cell treatment of tumor diseases, and can directly kill tumor cells and adjust the immunity of the body when re-infused into the body.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to a technique for amplifying immune cells by combining extracts of traditional Chinese medicines and cytokines and an application thereof. technical background [0002] Malignant tumors have become the number one cause of death in China, seriously threatening the health and lives of the people. At present, the treatment of tumors usually uses a combination of surgery, radiotherapy, chemotherapy, and biological therapy. Adjuvant chemotherapy is one of the important treatment methods for postoperative cancer patients, but chemotherapy often produces severe gastrointestinal toxicity, cardiotoxicity, neurotoxicity and other toxic reactions, and some patients have poor tolerance to chemotherapy drugs, making it difficult to adhere to the entire course of chemotherapy is an important factor limiting the therapeutic effect. [0003] In recent years, with the rapid developm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0784C12N5/0783A61P35/00
Inventor 吴晓星陈文珍焦明超王玉强
Owner 杭州特马赛生物技术有限公司
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