A kind of bacterial nano magnetosome-lectin complex and its preparation method and application
A composite and nano-magnetic technology, applied in the field of separation and purification, can solve the problems of poor particle size uniformity of nanoparticles, complicated preparation of epoxidized magnetic nanoparticles, etc., achieve good uniformity and dispersion, shorten preparation time, and improve efficiency. Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0042] Example 1 Preparation of BMPs-conA complex
[0043] BMPs surface modification: Weigh 1 mg BMPs and heat in 100 μL 1% SDS solution at 100°C for 5 minutes to remove BMPs surface proteins; collect BMPs on a magnetic stand and wash 3 times with 100 mM phosphate (PB, pH=7.5) buffer; Afterwards, BMPs were resuspended in 20 mg / mL PE solution, ultrasonicated at 100 W for 10 min; after incubation in the dark for 2 h, the BMPs were washed three times as above. That is, the BMPs-PE complex is obtained.
[0044] BMPs-BS 3 Preparation of the complex: draw 900 μL of 4mM PB buffer to suspend 1 mg of the complex obtained in the previous step, and disperse it with 80W ultrasonic; weigh BS 3 0.572mg, fully dissolved in 100μL distilled water, then added to BMPs suspension, 37°C, 200rpm, reacted for 0.5h; the BMPs complex after the reaction of the magnetic frame collector, removed the supernatant, suspended in 100mM PB buffer, dispersed by 80w ultrasonic wave, and then used Collect by m...
Embodiment 2
[0050] Example 2 Preparation of BMPs-wheat germ agglutinin (WGA) complex
[0051] Prepare BMPs-wheat germ agglutinin (WGA) complexes as follows:
[0052] (1) BMPs surface modification: Weigh 1mg of BMPs, add 50uL of 2% CTAB solution, heat treatment at 60°C for 20min to remove the protein on the surface of BMPs, then centrifuge to collect BMPs, wash and resuspend in 1mg / mL Phosphatidylserine PS solution, 80W ultrasound for 1h, incubate in the dark for 24h, and wash 3 times with 40mM phosphate (PB, pH=7) buffer solution to obtain BMPs-PS complex;
[0053] (2) Preparation of BMPs-glutaraldehyde complex: Take 1 mg of BMPs-PS complex, suspend it with 100 uL of 10 mM buffer solution, disperse it by ultrasonic, then add glutaraldehyde solution containing 0.2 mg glutaraldehyde, and in 4 Shake at -40°C for 10-60 minutes, collect, and obtain the BMPs-glutaraldehyde complex;
[0054] (3) Preparation of BMPs-WGA complex: prepare 0.1mg / ml wheat germ agglutinin solution, add 1mg BMPs-glut...
Embodiment 3
[0055] Example 3 BMPs-lentil agglutinin (LCA)
[0056] Prepare BMPs-LCA complexes as follows:
[0057] BMPs surface modification: Weigh 1mg of BMPs, add 100mL of 0.5% CHAPS solution, shake for 2 hours to remove the protein on the surface of BMPs, then collect BMPs by centrifugation, wash and resuspend in 100mg / mL PE solution, 100W ultrasonic 30min, incubate in the dark, and wash 3 times with 120mM phosphate (PB, pH=9) buffer solution to obtain the BMPs-PE complex;
[0058] Preparation of BMPs-dimethyladipimide complex: take 1mg of BMPs-PE complex, suspend it with 10mL of 6mM pH7 PB buffer, disperse it by ultrasonic, then add 10mg of dimethyladipimide The dimethyl adipimide solution of the amine was shaken and reacted at 4-40°C, and collected by centrifugation to obtain the BMPs-dimethyl adipimide complex;
[0059] Preparation of BMPs-LCA complex: prepare 10 mg / ml lentil agglutinin solution, add 1 mg BMPs-dimethyladipimide complex to 10 mL wheat germ adipimide solution, suspe...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com